Introduction to Theories of Several Super-resolution Fluorescence Microscopy Methods and Recent Advance in The Field*

Lv, Zhijian; Lu, Jingze; WU, Ya-Qiong; Liang-yi, Chen

doi:10.3724/sp.j.1206.2009.01626

Cited by 2 publications

(6 citation statements)

References 27 publications

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“…Specific procedure for FPALM is [39,43]: a few proteins tagged by PA-GFP are activated with low intensity (mW/cm 2 ) 405 nm laser pulse; then a 561 nm continuous wave stimulates molecules, although this results in a low amount of fluorescent emission because only a small number of elements were previously activated. By calculating and positioning the centers of these molecules their precise location can be obtained.…”

Section: Stochastic Excitation Microscopymentioning

confidence: 99%

“…However, two factors still influence measuring errors. Firstly, the accuracy of the cylindrical lens is limited penetration depths of about 600 nm; secondly, as depth increases there is an increase in the extent of optical aberrations along the Z-axis and a steady deterioration of the image [43].…”

Section: Three-dimensional Storm/palm Imaging Technologymentioning

confidence: 99%

“…The method can be adapted to single molecular imaging technologies. In order to resolve the bottleneck of low efficiency in photon collection and the difficulties in producing a SLM, we can add more phase modulators and closed-loop correction devices to enhance both the signalto-noise ratio and resolution [43,75].…”

Section: Three-dimensional Storm/palm Imaging Technologymentioning

confidence: 99%

“…Double-PALM simultaneously sends two different focal data sets to a CCD in order to extract information for three-dimensional image reconstruction. Currently, the lateral resolution has reached 30 nm, axial resolution 75 nm and an imaging depth of about 1 µm [43]. The illumination is divided into two beams by a 50:50 splitter and one of beam transits directly through the splitter, while the other is first reflected by the splitter and then reflected by a mirror.…”

Section: Three-dimensional Storm/palm Imaging Technologymentioning

confidence: 99%

“…Ye et al [5], Zhang et al [6] have been working on the living cells or single DNA molecules and several other research groups [7][8][9] in China have also been studying in the field of life sciences with the aid of fluorescence microscopy. In the latest 20 years, the following well-known methods: stimulated emission depletion microscopy (STED) , ground state depletion (GSD) [10,21,31,32], reversible saturable optical fluorescence transitions (RESOLFT) [15,16,33,34], saturated structured illumination microscopy (SSIM)/saturated pattern excitation microscopy (SPEM) [35][36][37][38], photo activated localization microscopy (PALM) [38][39][40][41][42][43][44][45][46][47], stochastic optical reconstruction microscopy (STORM) [48][49][50][51], ground state depletionindividual molecule return microscopy (GSDIM) [16,[52][53][54], and point accumulation for imaging in nanoscale topography (PAINT) [55][56][57][58], all these methods have successfully broken diffraction limits based on distinct physical processes.…”

mentioning

confidence: 99%

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Hacking the optical diffraction limit: Review on recent developments of fluorescence nanoscopy

Ding

Ren

2011

Chin. Sci. Bull.

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Subject to the diffraction limit, the resolution of conventional optical microscopy is constrained to about 200 and 500 nm in the lateral and axial planes, respectively. The advantage of optical microscopy in the life sciences over electronic microscopy, especially fluorescence microscopy, drives scientists to develop novel "hacks" to reach nanoscale resolutions by optical means. In this review, three aspects of the techniques are discussed: (1) lateral super-resolution; (2) axial super-resolution; (3) super-resolution in three dimensions. The principles of how the methods achieve the cross-barrier resolution are discussed, and recent advances in current techniques are described. With these methods, the use of fluorescence microscopy is growing quickly toward a new era: fluorescence nanoscopy that will reveal 2 orders of magnitude more information on cellular structure and dynamics. optical nanoscopy, fluorescence microscopy, diffraction limit Citation:Ding Y C, Xi P, Ren Q S. Hacking the optical diffraction limit: Review on recent developments of fluorescence nanoscopy.

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Section: Stochastic Excitation Microscopymentioning

confidence: 99%

Section: Three-dimensional Storm/palm Imaging Technologymentioning

confidence: 99%

Section: Three-dimensional Storm/palm Imaging Technologymentioning

confidence: 99%

Section: Three-dimensional Storm/palm Imaging Technologymentioning

confidence: 99%

mentioning

confidence: 99%

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Hacking the optical diffraction limit: Review on recent developments of fluorescence nanoscopy

Ding

Ren

2011

Chin. Sci. Bull.

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Bioimaging of Dissolvable Microneedle Arrays: Challenges and Opportunities

Yanni

Tao

et al. 2022

Research

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The emergence of microneedle arrays (MNAs) as a novel, simple, and minimally invasive administration approach largely addresses the challenges of traditional drug delivery. In particular, the dissolvable MNAs act as a promising, multifarious, and well-controlled platform for micro-nanotransport in medical research and cosmetic formulation applications. The effective delivery mostly depends on the behavior of the MNAs penetrated into the body, and accurate assessment is urgently needed. Advanced imaging technologies offer high sensitivity and resolution visualization of cross-scale, multidimensional, and multiparameter information, which can be used as an important aid for the evaluation and development of new MNAs. The combination of MNA technology and imaging can generate considerable new knowledge in a cost-effective manner with regards to the pharmacokinetics and bioavailability of active substances for the treatment of various diseases. In addition, noninvasive imaging techniques allow rapid, receptive assessment of transdermal penetration and drug deposition in various tissues, which could greatly facilitate the translation of experimental MNAs into clinical application. Relying on the recent promising development of bioimaging, this review is aimed at summarizing the current status, challenges, and future perspective on in vivo assessment of MNA drug delivery by various imaging technologies.

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Introduction to Theories of Several Super-resolution Fluorescence Microscopy Methods and Recent Advance in The Field*

Cited by 2 publications

References 27 publications

Hacking the optical diffraction limit: Review on recent developments of fluorescence nanoscopy

Hacking the optical diffraction limit: Review on recent developments of fluorescence nanoscopy

Bioimaging of Dissolvable Microneedle Arrays: Challenges and Opportunities

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