Introduction to the Physiology and Biochemistry of the Genus Clostridium

Andreesen, Jan R.; Bahl, Hubert; Gottschalk, Gerhard

doi:10.1007/978-1-4757-9718-3_2

Cited by 88 publications

(85 citation statements)

References 165 publications

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“…In the latter oxidation pathway, L-ornithine is first converted to D-ornithine by ornithine racemase, which has recently been purified and characterized by our group (3). This pathway is also found in C. subterminales (4).…”

supporting

confidence: 59%

Cloning, Sequencing, Heterologous Expression, Purification, and Characterization of Adenosylcobalamin-dependentd-Ornithine Aminomutase from Clostridium sticklandii

Chen¹,

Wu²,

Lin³

et al. 2001

Journal of Biological Chemistry

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supporting

confidence: 59%

Cloning, Sequencing, Heterologous Expression, Purification, and Characterization of Adenosylcobalamin-dependentd-Ornithine Aminomutase from Clostridium sticklandii

Chen¹,

Wu²,

Lin³

et al. 2001

Journal of Biological Chemistry

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“…The impressive metabolic capacity of the many different species has a high biotechnological potential (1,9). To better understand the molecular mechanisms involved in the regulation of metabolic pathways, several groups have started to analyze the genetics of clostridia (21,27).…”

mentioning

confidence: 99%

Purification and characterization of the DNA-dependent RNA polymerase from Clostridium acetobutylicum

Pich

Bahl

1991

J Bacteriol

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The DNA-dependent RNA polymerase (EC 2.7.7.6) from Clostridium acetobutylicum DSM 1731 has been purified to homogeneity and characterized. The purified enzyme was composed of four subunits and had a molecular mass of 370,000 Da. Western immunoblot analysis with polyclonal antibodies against the sigma 70 subunit of Escherichia coli RNA polymerase identified the 46,000-Da subunit as an immunologically and probably functionally related protein. The other three subunits of 128,000, 117,000, and 42,000 Da are tentatively analogous to the beta, beta', and alpha subunits, respectively, of other eubacterial RNA polymerases. The RNA polymerase activity was completely dependent on Mg2+, nucleoside triphosphates, and a DNA template. The presence of Mg2+ or Mn2+ in buffers used for purification or storage caused irreversible inactivation of the RNA polymerase.

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“…The relatively unstable and unpredictable biological hydrogen-production processes are primarily dependent on fermentation conditions such as pH (Fang and Liu, 2002;Zhu and Yang, 2004;Khanal et al, 2004) and hydraulic or solid retention time. Recent reports pointed out that Clostridium species were the dominant microorganisms in anaerobic hydrogen-fermentation processes (Iyer et al, 2004;Andreesen et al, 1989;Wang and Wan, 2009;Cebeci and Sonmez, 2006), but their contributions in hydrogen production have not yet been identified quantitatively.…”

Section: Reformation (Steam Reforming) Of Hydrogen-richmentioning

confidence: 99%

Applications of the Box-Wilson Design Model for Bio-hydrogen Production using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564)

Alalayah

Kalil²,

Kadhum³

et al. 2010

Pakistan J. of Biological Sciences

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Box-Wilson Design (BWD) model was applied to determine the optimum values of influencing parameters in anaerobic fermentation to produce hydrogen using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564). The main focus of the study was to find the optimal relationship between the hydrogen yield and three variables including initial substrate concentration, initial medium pH and reaction temperature. Microbial growth kinetic parameters for hydrogen production under anaerobic conditions were determined using the Monod model with incorporation of a substrate inhibition term. The values of µ (maximum specific max growth rate) and K (saturation constant) were 0.398 hG and 5.509 g LG , respectively, using glucose as the substrate concentration, 37°C reaction temperature and 6.0±0.2 initial medium pH gave 80% of the predicted maximum yield of hydrogen where as the experimental yield obtained in this study was 77.75% exhibiting a close accuracy between estimated and experimental values. This is the first report to predict bio-hydrogen yield by applying Box-Wilson Design in anaerobic fermentation while optimizing the effects of environmental factors prevailing there by investigating the effects of environmental factors.

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Introduction to the Physiology and Biochemistry of the Genus Clostridium

Cited by 88 publications

References 165 publications

Cloning, Sequencing, Heterologous Expression, Purification, and Characterization of Adenosylcobalamin-dependentd-Ornithine Aminomutase from Clostridium sticklandii

Cloning, Sequencing, Heterologous Expression, Purification, and Characterization of Adenosylcobalamin-dependentd-Ornithine Aminomutase from Clostridium sticklandii

Purification and characterization of the DNA-dependent RNA polymerase from Clostridium acetobutylicum

Applications of the Box-Wilson Design Model for Bio-hydrogen Production using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564)

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