Introduction to the ‘long COVID’ special issue

Szabó, Sándor; Gyires, Klara; Zayachkivska, Оksana

doi:10.1007/s10787-023-01197-x

Cited by 2 publications

(4 citation statements)

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“…Method validation is a mandatory requirement for analytical method development according to the guidelines issued by ICH, 17 USP, 21 FDA, 16 International Organization for Standardization ISO/IEC-17025, 22 and EDQM. 23 It is a continual improvement process that ensures the scope of the developed analytical method for evaluating its intended usage efficacy.…”

Section: Resultsmentioning

confidence: 99%

“…Accuracy refers to the proximity of an analytical method’s results to its parent values. According to ICH 17 and USP 21 guidelines for method validation, recovery (%) depends on the spiking concentration of the fortificant. According to the standards formulated by USP 21 and findings of Addis and Abebaw, 27 an analytical method should have 70–150% accuracy and <20% RSD during its validation.…”

Section: Resultsmentioning

confidence: 99%

“…According to ICH 17 and USP 21 guidelines for method validation, recovery (%) depends on the spiking concentration of the fortificant. According to the standards formulated by USP 21 and findings of Addis and Abebaw, 27 an analytical method should have 70–150% accuracy and <20% RSD during its validation. Results of the current study presented a range of percent recoveries ±S.D.…”

Section: Resultsmentioning

confidence: 99%

“…According to the FDA 16 /ICH 17 /USP 21 guidelines, LOD and LOQ are required parameters to determine the scope of any analytical method. According to the definitions of ISO/IEC-17025 22 and Vert et al, 29 LOD is the least detectable net concentration/true value of an analyte, whereas LOQ is the minimum amount of an analyte that can be quantitatively determined with suitable precision and accuracy.…”

Section: Resultsmentioning

confidence: 99%

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Rapid Method for Quantification of Iron (Fe⁺³) from Ferrazone (NaFe-EDTA) in Fortified Wheat Flour

Ahmad¹,

Mehmood²,

Khan³

et al. 2023

ACS Omega

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Conventional methods for quantifying the added iron in wheat flour are time-consuming and costly. A rapid method (Time/Sample: 95 min) was developed by modifying the conventional standard method (Time/Sample: 560 min) and validated. Linearity and linear regression of the rapid method presented excellent correlation coefficient (R 2 ) values (0.9976 to 0.9991), which were close to 1, while the limits of agreement (LOA) were in the range of −0.01 to 0.06 mg/kg. The limits of detection (LOD)/specificity and limits of quantitation (LOQ)/ sensitivity values were found to be 0.03 and 0.09 mg/kg, respectively. The rapid method was subjected to validation, wherein the precision of intra-assay, inter-assay, and inter-person was determined to be within the range of 1.35−7.25%. These results indicate a high level of accuracy and precision of the method. The percent relative standard deviation (RSD) for recoveries at varying spiking levels, that is, 5, 10, and 15 mg/kg, was determined at 1.33 lying far below the upper limit of acceptability (RSD < 20). Overall, the developed rapid method can be sustainably alternate for conventional methods owing to its ability to produce accurate, precise, robust, and reproducible results.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Rapid Method for Quantification of Iron (Fe⁺³) from Ferrazone (NaFe-EDTA) in Fortified Wheat Flour

Ahmad¹,

Mehmood²,

Khan³

et al. 2023

ACS Omega

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Virus-induced brain pathology and the neuroinflammation-inflammation continuum: the neurochemists view

Sian-Hulsmann,

Riederer

2024

J Neural Transm

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Fascinatingly, an abundance of recent studies has subscribed to the importance of cytotoxic immune mechanisms that appear to increase the risk/trigger for many progressive neurodegenerative disorders, including Parkinson’s disease (PD), Alzheimer’s disease (AD), amyotrophic lateral sclerosis, and multiple sclerosis. Events associated with the neuroinflammatory cascades, such as ageing, immunologic dysfunction, and eventually disruption of the blood–brain barrier and the “cytokine storm”, appear to be orchestrated mainly through the activation of microglial cells and communication with the neurons. The inflammatory processes prompt cellular protein dyshomeostasis. Parkinson’s and Alzheimer’s disease share a common feature marked by characteristic pathological hallmarks of abnormal neuronal protein accumulation. These Lewy bodies contain misfolded α-synuclein aggregates in PD or in the case of AD, they are Aβ deposits and tau-containing neurofibrillary tangles. Subsequently, these abnormal protein aggregates further elicit neurotoxic processes and events which contribute to the onset of neurodegeneration and to its progression including aggravation of neuroinflammation. However, there is a caveat for exclusively linking neuroinflammation with neurodegeneration, since it’s highly unlikely that immune dysregulation is the only factor that contributes to the manifestation of many of these neurodegenerative disorders. It is unquestionably a complex interaction with other factors such as genetics, age, and environment. This endorses the “multiple hit hypothesis”. Consequently, if the host has a genetic susceptibility coupled to an age-related weakened immune system, this makes them more susceptible to the virus/bacteria-related infection. This may trigger the onset of chronic cytotoxic neuroinflammatory processes leading to protein dyshomeostasis and accumulation, and finally, these events lead to neuronal destruction. Here, we differentiate “neuroinflammation” and “inflammation” with regard to the involvement of the blood–brain barrier, which seems to be intact in the case of neuroinflammation but defect in the case of inflammation. There is a neuroinflammation-inflammation continuum with regard to virus-induced brain affection. Therefore, we propose a staging of this process, which might be further developed by adding blood- and CSF parameters, their stage-dependent composition and stage-dependent severeness grade. If so, this might be suitable to optimise therapeutic strategies to fight brain neuroinflammation in its beginning and avoid inflammation at all.

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Introduction to the ‘long COVID’ special issue

Cited by 2 publications

References 0 publications

Rapid Method for Quantification of Iron (Fe⁺³) from Ferrazone (NaFe-EDTA) in Fortified Wheat Flour

Rapid Method for Quantification of Iron (Fe⁺³) from Ferrazone (NaFe-EDTA) in Fortified Wheat Flour

Virus-induced brain pathology and the neuroinflammation-inflammation continuum: the neurochemists view

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Introduction to the ‘long COVID’ special issue

Cited by 2 publications

References 0 publications

Rapid Method for Quantification of Iron (Fe+3) from Ferrazone (NaFe-EDTA) in Fortified Wheat Flour

Rapid Method for Quantification of Iron (Fe+3) from Ferrazone (NaFe-EDTA) in Fortified Wheat Flour

Virus-induced brain pathology and the neuroinflammation-inflammation continuum: the neurochemists view

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Product

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Rapid Method for Quantification of Iron (Fe⁺³) from Ferrazone (NaFe-EDTA) in Fortified Wheat Flour

Rapid Method for Quantification of Iron (Fe⁺³) from Ferrazone (NaFe-EDTA) in Fortified Wheat Flour