Biocrusts covering drylands account for major fractions
of terrestrial
biological nitrogen fixation and release large amounts of gaseous
reactive nitrogen (N
r
) as nitrous acid (HONO) and nitric
oxide (NO). Recent investigations suggested that aerobic and anaerobic
microbial nitrogen transformations occur simultaneously upon desiccation
of biocrusts, but the spatio-temporal distribution of seemingly contradictory
processes remained unclear. Here, we explore small-scale gradients
in chemical concentrations related to structural characteristics and
organism distribution. X-ray microtomography and fluorescence microscopy
revealed mixed pore size structures, where photoautotrophs and cyanobacterial
polysaccharides clustered irregularly in the uppermost millimeter.
Microsensor measurements showed strong gradients of pH, oxygen, and
nitrite, nitrate, and ammonium ion concentrations at micrometer scales
in both vertical and lateral directions. Initial oxygen saturation
was mostly low (∼30%) at full water holding capacity, suggesting
widely anoxic conditions, and increased rapidly upon desiccation.
Nitrite concentrations (∼6 to 800 μM) and pH values (∼6.5
to 9.5) were highest around 70% WHC. During further desiccation they
decreased, while emissions of HONO and NO increased, reaching maximum
values around 20% WHC. Our results illustrate simultaneous, spatially
separated aerobic and anaerobic nitrogen transformations, which are
critical for N
r
emissions, but might be impacted by future
global change and land management.