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The article contains sections titled: 1. Ecology 1.1. Laundry, Wastewater, and the Environment 1.2. Contribution of Laundry to the Sewage Load 1.3. Detergent Laws 1.3.1. Development of the European Detergent Legislation 1.3.2. Regulatory Limitations on Anionic and Nonionic Surfactants 1.3.3. Primary Biodegradation Test Procedures 1.3.4. Regulation of Maximum Phosphate Content in Detergents 1.4. General Criteria for the Ecological Evaluation of Detergent Chemicals 1.4.1. Concept 1.4.2. Environmental Exposure Assessment 1.4.2.1. Biodegradation 1.4.2.2. Biodegradability Standard Test Methods 1.4.2.3. Supplementary Biodegradation Test Methods 1.4.2.4. Exposure Analysis 1.4.3. Assessment of Environmental Effects 1.4.3.1. Basic Ecotoxicity Tests 1.4.3.2. Subchronic and Chronic Ecotoxicity Tests 1.4.3.3. Biocenotic Ecotoxicity Tests 1.4.3.4. Bioaccumulation 1.4.4. Process of Environmental Risk Assessment 1.5. Ecological Characterization of Main Detergent Ingredients 1.5.1. Surfactants 1.5.1.1. Anionic Surfactants 1.5.1.2. Nonionic Surfactants 1.5.1.3. Cationic Surfactants 1.5.2. Builders 1.5.2.1. Zeolites 1.5.2.2. Polycarboxylates 1.5.2.3. Citrates 1.5.2.4. Sodium Carbonate (Soda Ash) 1.5.2.5. Nitrilotriacetate (NTA) 1.5.3. Bleaching Agents 1.5.3.1. Sodium Perborate 1.5.3.2. Sodium Percarbonate 1.5.3.3. Tetraacetylethylenediamine (TAED) 1.5.4. Auxiliary Agents 1.5.4.1. Phosphonates 1.5.4.2. EDTA 1.5.4.3. Enzymes 1.5.4.4. Optical Brighteners 1.5.4.5. Carboxymethyl Cellulose 1.5.4.6. Dye Transfer Inhibitors 1.5.4.7. Fragrances 1.5.4.8. Foam Regulators 1.5.4.9. Soil Repellents 1.5.4.10. Dyes 1.5.4.11. Sodium Sulfate 2. Toxicology 2.1. Detergent Ingredients 2.1.1. Surfactants 2.1.2. Builders 2.1.3. Bleach‐Active Compounds 2.1.4. Auxiliary Agents 2.2. Finished Detergents 2.3. Conclusions
The article contains sections titled: 1. Ecology 1.1. Laundry, Wastewater, and the Environment 1.2. Contribution of Laundry to the Sewage Load 1.3. Detergent Laws 1.3.1. Development of the European Detergent Legislation 1.3.2. Regulatory Limitations on Anionic and Nonionic Surfactants 1.3.3. Primary Biodegradation Test Procedures 1.3.4. Regulation of Maximum Phosphate Content in Detergents 1.4. General Criteria for the Ecological Evaluation of Detergent Chemicals 1.4.1. Concept 1.4.2. Environmental Exposure Assessment 1.4.2.1. Biodegradation 1.4.2.2. Biodegradability Standard Test Methods 1.4.2.3. Supplementary Biodegradation Test Methods 1.4.2.4. Exposure Analysis 1.4.3. Assessment of Environmental Effects 1.4.3.1. Basic Ecotoxicity Tests 1.4.3.2. Subchronic and Chronic Ecotoxicity Tests 1.4.3.3. Biocenotic Ecotoxicity Tests 1.4.3.4. Bioaccumulation 1.4.4. Process of Environmental Risk Assessment 1.5. Ecological Characterization of Main Detergent Ingredients 1.5.1. Surfactants 1.5.1.1. Anionic Surfactants 1.5.1.2. Nonionic Surfactants 1.5.1.3. Cationic Surfactants 1.5.2. Builders 1.5.2.1. Zeolites 1.5.2.2. Polycarboxylates 1.5.2.3. Citrates 1.5.2.4. Sodium Carbonate (Soda Ash) 1.5.2.5. Nitrilotriacetate (NTA) 1.5.3. Bleaching Agents 1.5.3.1. Sodium Perborate 1.5.3.2. Sodium Percarbonate 1.5.3.3. Tetraacetylethylenediamine (TAED) 1.5.4. Auxiliary Agents 1.5.4.1. Phosphonates 1.5.4.2. EDTA 1.5.4.3. Enzymes 1.5.4.4. Optical Brighteners 1.5.4.5. Carboxymethyl Cellulose 1.5.4.6. Dye Transfer Inhibitors 1.5.4.7. Fragrances 1.5.4.8. Foam Regulators 1.5.4.9. Soil Repellents 1.5.4.10. Dyes 1.5.4.11. Sodium Sulfate 2. Toxicology 2.1. Detergent Ingredients 2.1.1. Surfactants 2.1.2. Builders 2.1.3. Bleach‐Active Compounds 2.1.4. Auxiliary Agents 2.2. Finished Detergents 2.3. Conclusions
Polyalcohol ethoxylate (PAE), an anionic surfactant, is the primary component in most laundry and dish wash detergents and is therefore highly loaded in domestic wastewater. Its biodegradation results in the formation of several metabolites and the fate of these metabolites through wastewater treatment plants, graywater recycling processes, and in the environment must be clearly understood. Biodegradation pathways for PAE were investigated in this project with a municipal wastewater microbial consortium. A microtiter-based oxygen sensor system was utilized to determine the preferential use of potential biodegradation products. Results show that while polyethylene glycols (PEGs) were readily degraded by PAE acclimated microorganisms, most of the carboxylic acids tested were not degraded. Biodegradation of PEGs suggests that hydrophobe-hydrophile scission was the dominant pathway for PAE biodegradation in this wastewater community. Ethylene glycol (EG) and diethylene glycol (DEG) were not utilized by microbial populations capable of degrading higher molecular weight EGs. It is possible that EG and DEG may accumulate. The microtiter-based oxygen sensor system was successfully utilized to elucidate information on PAE biodegradation pathways and could be applied to study biodegradation pathways for other important contaminants.
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