Introduction to Light Scattering by Biological Objects

Khlebtsov, Nikolai G.; Maksimova, Irina L.; Meglinski, Igor; Wang, LIhong V.; Tuchin, Valery V.

doi:10.1117/3.2219603.ch1

Cited by 25 publications

(48 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Note the higher birefringence values in the infarcted region with treatment protein properties in solutions [5], supplying a variety of nondestructive evaluation methods [6], developing advanced concepts such as polarization entropy [7], contributing to remote sensing in meteorology and astronomy [8,9], differentiating between normal and pre-cancerous cells [10], as well as other biomedical applications [11,12]. Traditional polarimetry is well suited for applications in optically clear media and for studies of surfaces; however, multiple scattering in optically thick turbid media such as most biological tissues causes extensive depolarization that confounds the established techniques.…”

Section: Biophotonicsmentioning

confidence: 99%

Mueller matrix decomposition for polarized light assessment of biological tissues

Ghosh¹,

Wood²,

Li³

et al. 2009

Journal of Biophotonics

151

117

View full text Add to dashboard Cite

The Mueller matrix represents the transfer function of an optical system in its interactions with polarized light and its elements relate to specific biologically or clinically relevant properties. However, when many optical polarization effects occur simultaneously, the resulting matrix elements represent several "lumped" effects, thus hindering their unique interpretation. Currently, no methods exist to extract these individual properties in turbid media. Here, we present a novel application of a Mueller matrix decomposition methodology that achieves this objective. The methodology is validated theoretically via a novel polarized-light propagation model, and experimentally in tissue simulating phantoms. The potential of the approach is explored for two specific biomedical applications: monitoring of changes in myocardial tissues following regenerative stem cell therapy, through birefringence-induced retardation of the light's linear and circular polarizations, and non-invasive blood glucose measurements through chirality-induced rotation of the light's linear polarization. Results demonstrate potential for both applications.

show abstract

Section: Biophotonicsmentioning

confidence: 99%

Mueller matrix decomposition for polarized light assessment of biological tissues

Ghosh¹,

Wood²,

Li³

et al. 2009

Journal of Biophotonics

151

117

View full text Add to dashboard Cite

show abstract

“…In general, its value has been found to range between 0.2 and 0.4 for soft tissues. 17 This is the range of T v we consider in this work.…”

Section: Numerical Comparisonsmentioning

confidence: 99%

“…22 The volume fraction occupied by particles is also known as the packing density and is a characteristic that can be measured experimentally. 17 The total volume fraction occupied by the spheres is estimated as T v =1−T w , where T w is the weight fraction of water in the tissue. 4 Measurements of T v for various soft tissues show that its value ranges between 0.2 and 0.4.…”

Section: Numerical Comparisonsmentioning

confidence: 99%

Volume concentration and size dependence of diffuse reflectance in a fractal soft tissue model

Sharma

Banerjee

2005

Medical Physics

View full text Add to dashboard Cite

Employing a fractal model for the particle size distribution, we examine the possibility of relating the size/volume concentration changes in the tissue to its diffuse reflectance. It is noted that for the practically interesting range of fractal dimension values alpha, the curves depicting the variation of diffuse reflectance (the ratio of diffuse reflectance at two suitably chosen source detector separations) with alpha at fixed volume concentration Tv, are single valued. The same is true if alpha is fixed and Tv is varied. This crucial observation shows that it should be possible to identify changes in the size/volume concentration of the tissue from the diffuse reflectance measurement at two source detector separations when either alpha or Tv is a priori known. Similar results have been obtained for ratio of fluence measured at two suitably chosen depths within the tissue.

show abstract

“…There is a great deal of interest currently in studies relating to light propagation in biomedical tissues (see, for example, [1,2] and references therein). It is felt that this technique holds great potential for tissue characterization in general and for early detection of cancers of soft tissues in particular [3][4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

On the validity of the anomalous diffraction approximation in light-scattering studies of soft biomedical tissues

Sharma

Banerjee

2012

Journal of Modern Optics

View full text Add to dashboard Cite

In simulations of light propagation in soft biomedical tissues, the tissue is generally modeled as a turbid medium. In one such model, suspended scatterers are assumed to be homogeneous spheres of size following a fractal size distribution law. In this paper, we examine, within the framework of this model, the accuracy of a well-known approximation known as the anomalous diffraction approximation. The deployment of this approximation allows the tissue scattering coefficient to be expressed in a closed analytic form. It is demonstrated that use of the anomalous diffraction approximation yields reasonably good results for the tissue scattering coefficient, the phase function and the asymmetry parameter. It is also shown that the experimentally observed wavelength dependence of the scattering coefficient follows analytically from the formulas obtained using this approximation. The results are important from the point of view of constructing more realistic tissue models which may include non-spherical scatterers.

show abstract

Introduction to Light Scattering by Biological Objects

Cited by 25 publications

References 0 publications

Mueller matrix decomposition for polarized light assessment of biological tissues

Mueller matrix decomposition for polarized light assessment of biological tissues

Volume concentration and size dependence of diffuse reflectance in a fractal soft tissue model

On the validity of the anomalous diffraction approximation in light-scattering studies of soft biomedical tissues

Contact Info

Product

Resources

About