Introduction to Fed-Batch Cultures

Lim, Henry C.; Shin, Hwa Sung

doi:10.1017/cbo9781139018777.002

Cited by 2 publications

(1 citation statement)

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“…Fed-batch fermentation has been widely employed for the enhanced production of desirable microbial metabolites. The fed-batch cultivation process is being carried out by intermittent or continuous addition of essential nutrients to the culture vessel for optimizing cell growth and maximization of product yield during fermentation [19]. In fed-batch cultivation processes, feeding of a substrate or carbon source at defined time intervals and concentrations is one of the most important factors for efficient conversion of substrate into the desired end product.…”

Section: Introductionmentioning

confidence: 99%

Metabolic Engineering of Pediococcus acidilactici BD16 for Heterologous Expression of Synthetic alaD Gene Cassette and L-Alanine Production in the Recombinant Strain Using Fed-Batch Fermentation

Sharma

Noda

Sugiyama

et al. 2021

Foods

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Metabolic engineering substantially aims at the development of more efficient, robust and industrially competitive microbial strains for the potential applications in food, fermentation and pharmaceutical industries. An efficient lab scale bioprocess was developed for high level fermentative production of L-alanine using metabolically engineered Pediococcus acidilactici BD16 (alaD+). Computational biology tools assisted the designing of a synthetic alaD gene cassette, which was further cloned in shuttle vector pLES003 and expressed using an auto-inducible P289 promoter. Further, L-alanine production in the recombinant P. acidilactici BD16 (alaD+) strain was carried out using fed-batch fermentation under oxygen depression conditions, which significantly enhanced L-alanine levels. The recombinant strain expressing the synthetic alaD gene produced 229.12 g/L of L-alanine after 42 h of fed-batch fermentation, which is the second highest microbial L-alanine titer reported so far. After extraction and crystallization, 95% crystal L-alanine (217.54 g/L) was recovered from the culture broth with an enantiomeric purity of 97%. The developed bioprocess using recombinant P. acidilactici BD16 (alaD+) is suggested as the best alternative to chemical-based commercial synthesis of L-alanine for potential industrial applications.

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Section: Introductionmentioning

confidence: 99%