Introduction to Electrochemically Active Biofilms

Beyenal, Haluk; Babauta, Jerome T.

doi:10.1002/9781119097426.ch1

Cited by 6 publications

(7 citation statements)

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“…This behavior suggests that any current generated by the biomass of electroactive 240 bacteria, including cable bacteria, was obscured during scans by current arising from irreversible redox reactions, such as oxidation of dissolved iron. The reduction peak was unidentifiable throughout the scan, a common phenomenon in benthic and sediment MFCs (Babauta and Beyenal, 2015b). Taken together, these results suggest that the electrode https://doi.org/10.5194/bg-2019-334 Preprint.…”

Section: Encouraging the Growth Of Cable Bacteria On Poised Electrodesmentioning

confidence: 65%

Inducing the Attachment of Cable Bacteria on Oxidizing Electrodes

Li¹,

Reimers²,

Alleau³

2019

Preprint

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The scope of the present study is to introduce electrochemical reactors as a tool for investigating the growth of novel filamentous cable bacteria and their unique extracellular electron transfer ability. New evidence that cable bacteria are widely distributed in sediments throughout an estuarine system connected to the NE Pacific Ocean is also presented. Cable bacteria found within Yaquina Bay, Oregon, USA, appear to cluster with the genus, Candidatus Electrothrix. Results of a 135-day bioelectrochemical reactor experiment confirm a previous observation that cable 10 bacteria can grow on oxidatively poised electrodes suspended in anaerobic seawater above reducing sediments.However, several diverse morphologies of Desulfobulbaceae filaments, cells, and colonies were observed on the carbon fibers of the suspended electrodes including encrusted chains of cells. These observations provide new information to suggest what conditions will induce cable bacteria to perform electron donation to an electrode surface, further informing future experiments to culture cable bacteria apart from a sediment matrix. 15 IntroductionLong distance electron transfer (LDET) is a mechanism used by certain microorganisms to generate energy through the transfer of electrons over distances greater than a cell-length. These microorganisms may pass electrons across dissolved redox shuttles, nanofiber-like cell appendages, outer-membrane cytochromes, and/or mineral nanoparticles to connect extracellular electron donors and acceptors Lovley, 2016). Recently, a novel type of LDET 20 exhibited by filamentous bacteria in the family of Desulfobulbaceae was discovered in the uppermost centimeters of various aquatic, but mainly marine, sediments (Malkin et al., 2014;Pfeffer et al., 2012). These filamentous bacteria, also known as "cable bacteria", electrically connect two spatially separated redox half-reactions and generate electrical current over distances that can extend to centimeters, which is an order of magnitude longer than previously recognized LDET distances (Meysman, 2017). 25The unique ability of cable bacteria to perform LDET creates a spatial separation of oxygen reduction in oxic surface layers of sediment from sulfide oxidation in subsurface layers (Meysman, 2017). The spatial separation of these two half-reactions also creates localized porewater pH extremes in oxic and sulfidic layers, which induces a series of secondary reactions that stimulate the geochemical cycling of elements such as iron, manganese, calcium, phosphorus, and nitrogen (Kessler et al., 2018;Rao et al., 2016; Seitaj et al., 2015;Sulu-Gambari et al., 2016b, 2016a. In addition 30 to altering established perceptions of sedimentary biogeochemical cycling and microbial ecology (Meysman, 2017;Nielsen and Risgaard-Petersen, 2015), cable bacteria also possess intriguing structural features that may inspire new engineering applications in areas of bioenergy harvesting and biomaterial design (Lovley, 2016). Pfeffer et al., 2012). Therefore to initiate this enquiry, sedimen...

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Section: Encouraging the Growth Of Cable Bacteria On Poised Electrodesmentioning

confidence: 65%

Inducing the Attachment of Cable Bacteria on Oxidizing Electrodes

Li¹,

Reimers²,

Alleau³

2019

Preprint

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“…A.4K.4; Rabaey et al, 2009; Babauta et al, 2015; Speers and Reguera, 2015). BES have gained considerable interest in the past few years due to their ability to harness the catalytic activity of anaerobic microorganisms for the production of commodity chemicals, bioen-ergy, and biofuels (Rabaey et al, 2009; Babauta et al, 2015; Speers and Reguera, 2015). The principles and operations of BES have been reviewed in detail in multiple previous publications (Rabaey et al, 2009; Babauta et al, 2015; Speers and Reguera, 2015), and the Commentary section of this appendix also provides an overview of the essential concepts and terminology in BES.…”

Section: Basic Protocol 4 Construction Of the Bioelectrochemical Sysmentioning

confidence: 99%

“…Electrochemically active biofilms grown on electrodes play a central role in the operational performance of BES (Franks et al, 2010; Babauta et al, 2015). Therefore, this appendix presents low-cost assembly and operation of a reusable BES.…”

Section: Commentarymentioning

confidence: 99%

“…Bioelectrochemical systems (Rabaey et al, 2009) (BES) have gained considerable interest in the past few years due to their ability to harness the catalytic activity of anaerobic microorganisms for the production of commodity chemicals, bioenergy, and biofuels (Logan and Rabaey, 2012;Lovley and Nevin, 2013). Electrochemically active biofilms grown on electrodes play a central role in the operational performance of BES (Franks et al, 2010;Babauta et al, 2015). Therefore, this appendix presents low-cost assembly and operation of a reusable BES.…”

Section: Bioelectrochemical Systems (Bes) and Microbial Electrolysis ...mentioning

confidence: 99%

“…Bioelectrochemical systems (BES) use microorganisms to catalyze an oxidation and/or reduction reaction at an anodic and/or cathodic electrode, respectively (Fig. A.4K.4;Rabaey et al, 2009;Babauta et al, 2015;Speers and Reguera, 2015). BES have gained considerable interest in the past few years due to their ability to harness the catalytic activity of anaerobic microorganisms for the production of commodity chemicals, bioenergy, and biofuels (Rabaey et al, 2009;Babauta et al, 2015;Speers and Reguera, 2015).…”

Section: Construction Of the Bioelectrochemical System (Bes)mentioning

confidence: 99%

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A Simple and Low‐Cost Procedure for Growing Geobacter sulfurreducens Cell Cultures and Biofilms in Bioelectrochemical Systems

O’Brien

Malvankar

2016

CP Microbiology

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Anaerobic microorganisms play a central role in several environmental processes and regulate global biogeochemical cycling of nutrients and minerals. Many anaerobic microorganisms are important for the production of bioenergy and biofuels. However, the major hurdle in studying anaerobic microorganisms in the laboratory is the requirement for sophisticated and expensive gassing stations and glove boxes to create and maintain the anaerobic environment. This appendix presents a simple design for a gassing station that can be used readily by an inexperienced investigator for cultivation of anaerobic microorganisms. In addition, this appendix also details the low-cost assembly of bioelectrochemical systems and outlines a simplified procedure for cultivating and analyzing bacterial cell cultures and biofilms that produce electric current, using Geobacter sulfurreducens as a model organism.

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Microbial Electrochemical Technologies

2023

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Introduction to Electrochemically Active Biofilms

Cited by 6 publications

References 204 publications

Inducing the Attachment of Cable Bacteria on Oxidizing Electrodes

Inducing the Attachment of Cable Bacteria on Oxidizing Electrodes

A Simple and Low‐Cost Procedure for Growing Geobacter sulfurreducens Cell Cultures and Biofilms in Bioelectrochemical Systems

Microbial Electrochemical Technologies

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