Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower

Pavlović, Suzana; Savić﻿﻿﻿, Jelena; Milojević, Jelena; Vinterhalter, Branka; Girek, Zdenka; Adžić, Slađan; Zecevic, B.; Banjac, Nevena

doi:10.1007/s11240-020-01948-6

Cited by 6 publications

(4 citation statements)

References 63 publications

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“…The salt resistance of the new series in vitro was experimented on with growing salinity, and improved salt resistance was demonstrated. 62 This study provides an encouraging way to cultivate salt-tolerant plants and escorts an increase in grain production.…”

Section: The Role Of Protein Kinases In Salt Stressmentioning

confidence: 89%

“…They performed Agrobacterium -mediated transformation to strengthen salt tolerance of the genetically modified cauliflower series. The salt resistance of the new series in vitro was experimented on with growing salinity, and improved salt resistance was demonstrated . This study provides an encouraging way to cultivate salt-tolerant plants and escorts an increase in grain production.…”

Section: Protein Kinases As Potential Targets For Abiotic Stress Tole...mentioning

confidence: 99%

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Protein Kinases as Potential Targets Contribute to the Development of Agrochemicals

Sun

Wang

Gao

et al. 2023

J. Agric. Food Chem.

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Using agrochemicals against pest insects, fungi, and weeds plays a major part in maintaining and improving crop yields, which helps to solve the issue of food security. Due to the limited targets and resistance of agrochemicals, protein kinases are regarded as attractive potential targets to develop new agrochemicals. Recently, a lot of investigations have shown the extension of agrochemicals by targeting protein kinases, implying an increasing concern for this kind of method. However, few people have summarized and discussed the targetability of protein kinases contributing to the development of agrochemicals. In this work, we introduce the research on protein kinases as potential targets used in crop protection and discuss the prospects of protein kinases in the field of agrochemical development. This study may not only provide guidance for the contribution of protein kinases to the development of agrochemicals but also help nonprofessionals such as students learn and understand the role of protein kinases quickly.

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Section: The Role Of Protein Kinases In Salt Stressmentioning

confidence: 89%

Section: Protein Kinases As Potential Targets For Abiotic Stress Tole...mentioning

confidence: 99%

Protein Kinases as Potential Targets Contribute to the Development of Agrochemicals

Sun

Wang

Gao

et al. 2023

J. Agric. Food Chem.

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“…The conditions for plant regeneration in vitro in numerous members of the genus Brassica have been intensively studied in recent decades (reviewed in [32][33][34]). Somatic embryogenesis was described in B. napus [35,36], B. oleracea [37][38][39][40], B. rapa [41], B. carinata [42,43], B. repanda [44], and B. juncea [45] using immature zygotic embryos, hypocotyls, cotyledons, leaves or roots as source plant material.…”

Section: In Vitro Growth and Regeneration As A Biotechnological Appro...mentioning

confidence: 99%

In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research

et al. 2023

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Kohlrabi (Brassica oleracea var. gongylodes) is a vegetable cultivated for its edible stem tuber. Although valued for its nutritional properties and tolerance to abiotic stress, kohlrabi is one of the least studied brassicas. In this review, we summarize the results of our decade-long research on in vitro morphogenesis of kohlrabi, starting from 2013. Protocols for efficient in vitro regeneration with minimal requirements for external application of plant growth regulators (PGRs) have been developed, both by somatic embryogenesis (SE) and by de novo shoot organogenesis (DNSO). Efficient regeneration by direct SE was achieved using immature zygotic embryos as explants incubated on PGR-free media, and the production process was maintained in culture thanks to highly efficient secondary SE. Conversely, efficient regeneration by indirect DNSO was achieved using entire seedlings as explants and adding only cytokinin (CK) without the need for exogenous auxin. Comprehensive phytohormone analyses revealed that different exogenously applied CKs differentially affected the composition of endogenous phytohormones and induced changes in the expression of cell cycle-related genes and other genes involved in the organogenic response. The addition of high sucrose concentrations to the nutrient media failed to induce the formation of stem tubers in in vitro culture, but revealed a complex interaction with exogenously applied CKs, interfering with both the endogenous phytohormonome and the expression of organogenesis-related genes. Our work has provided substantial biotechnological advances in the field of in vitro regeneration of kohlrabi, as well as in understanding the underlying phytohormonal regulation. The review aims to introduce kohlrabi to the scientific community as a model system for both basic and applied research, while we continue seeking answers to the outstanding questions and trying to pave the way for the development of more resistant varieties.

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“…Direct somatic embryogenesis has been reported for Brassica campestris (Soma & Sikdar 2005), Brassica napus (Burbulis & Kupriene 2005;Abdollahi et al 2012), Brassica oleracea (Pavlović et al 2013), Brassica juncea (Kumari et al 2000;Faisal et al 2021) and Arabidopsis thaliana (Gaj 2001;Kurczyjska et al 2007). Successful genetic modifications have been https://doi.org/10.17221/26/2023-CJGPB achieved using embryogenic culture (Von Arnold et al 2002;Pavlović et al 2020).…”

mentioning

confidence: 99%

Induction of direct somatic embryogenesis and genetic stability of somatic embryo-derived plants of broccoli

Pavlović,

Damnjanović,

Girek

et al. 2023

CZECH J GENET PLANT

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The influence of the developmental stage of zygotic embryos and the composition and pH of the Gamborg induction medium B5 on the initiation and development of somatic embryos was investigated. The optimal medium was B5 medium with a pH value of 5.0 and without plant growth regulator, at which the highest frequency of somatic embryogenesis (56.67%) and the highest average number of somatic embryos per explant (3.35) were achieved. Somatic embryos appeared directly on the hypocotyls of the explants, without the callus stage. On zygotic embryos in the early cotyledonary phase, three times higher regeneration was achieved compared to larger embryos in the cotyledonary phase. The induction of somatic embryogenesis did not occur during the growth of explants on the medium containing 2,4-dichlorophenoxyacetic acid, nor on zygotic embryos in the late cotyledonary phase. Random amplified polymorphic DNA analysis showed the genetic stability of somatic embryo-derived plants, which makes this newly established protocol suitable for the regeneration and propagation of desirable broccoli genotypes.

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Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower

Cited by 6 publications

References 63 publications

Protein Kinases as Potential Targets Contribute to the Development of Agrochemicals

Protein Kinases as Potential Targets Contribute to the Development of Agrochemicals

In Vitro Growth and Regeneration of Brassica oleracea var. gongylodes: A Decade of Research

Induction of direct somatic embryogenesis and genetic stability of somatic embryo-derived plants of broccoli

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