Introduction of in Vitro Grapes of Interspecific Origin

Abstract

During clonal micro-propagation of grapes of interspecific origin of the Aleshenkin variety, it was revealed that the optimal type of explants for initiating a sterile culture are meristematic apexes 100 – 150 μm in height, which are advisable to be planted in test tubes on a nutrient medium according to the prescription of Quorin Lepuavr (QL) (Quoirin & Lepoivre, 1977) enriched with the following substances (mg/l): thiamine (B1), pyridoxine (B6), nicotinic acid (PP) – 0.5 each; 6-BAP – 0.1; inositol – 100; sucrose – 30,000, agar-agar – 7,000 and incubate for 70 days in a light room at an illumination intensity of 2,500 lux, a 16-hour photoperiod and a temperature of 20 – 22 C. At the multiplication stage, with two consecutive passages of 40 days of sub-culturing on a nutrient medium according to the prescription of Murashige and Skoog (MS) (Murashige & Skoog, 1962), enriched with the following substances (mg/l): thiamine (B1), pyridoxine (B6), nicotinic acid (PP) – 0.5 each; 6-BAP – 0.1, inositol – 100; sucrose – 30,000, agar-agar – 7,000, the advantage of this type of explants has been shown. Since in terms of the coefficient of multiplication, regenerant plants introduced into culture in vitro by meristematic apexes after the first passage were 2.3 times and after the second passage 1.4 times higher than the plants introduced into culture by micro-cuttings.