Introduction of a nitrogen-fixing cyanobacterium into tobacco shoot regenerates

Гусев, М. В.; Korzhenevskaya, T. G.; Pyvovarova, L. V.; Baulina, O. I.; Butenko, R. G.

doi:10.1007/bf00446361

Cited by 25 publications

(10 citation statements)

References 24 publications

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“…The extension of the range of plants with the ability to fix nitrogen has been a long-term objective of plant scientists, which can have great environmental and economic significance. In this context, cyanobacteria are particularly suitable for the creation of novel nitrogen-fixing associations since they already form a wide variety of natural symbiotic associations (Gusev et al 1986;Rai 1990;Rai et al 2000). Nilsson et al (2002Nilsson et al ( , 2005Nilsson et al ( , 2006 tested numerous symbiotic cyanobacterial isolates for their ability to associate with rice and demonstrated that symbiotically competent Nostoc strains respond chemotactically to extracts of natural host and non-host plants.…”

Section: Resultsmentioning

confidence: 98%

Physiological characterization and electron microscopic investigation of cyanobacteria associated with wheat rhizosphere

et al. 2009

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Physiological attributes of a set of cyanobacterial strains, isolated from the rhizosphere of wheat (var. HD 2687), identified as belonging to the genera Calothrix (n = 3), Westiellopsis (1), Hapalosiphon (2) and Nostoc (2), were axenized and evaluated. The concentrated culture filtrates of three cyanobacterial strains - C. ghosei, H. intricatus and Nostoc sp. were able to enhance germination percentage, radicle and coleoptile length in inhibition experiments with wheat seeds. Indole-3-acetic acid (IAA) production was recorded in light and dark (+0.5 % glucose) incubated cultures. Incubation in the presence of tryptophan significantly enhanced IAA production. Acetylene-reducing activity was higher in light incubated cultures of Nostoc sp. followed by C. ghosei, while in the dark, C. ghosei recorded highest values. TLC of the filtrates revealed the presence of several amino acids such as histidine, and auxin-like compounds. Co-culturing with selected strains recorded significant enhancement in plant chlorophyll. Root sections of wheat seedlings co-cultured with C. ghosei revealed the presence of short filaments inside the root hairs and cortical region. Such strains can be promising candidates for developing plant growth promoting associations for wheat crop, besides serving as model systems for understanding the metabolic interactions of cyanobacteria with host plant, such as wheat.

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Section: Resultsmentioning

confidence: 98%

Physiological characterization and electron microscopic investigation of cyanobacteria associated with wheat rhizosphere

et al. 2009

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“…Cyanobacteria have been mixed with Daucus cells (Bradley, 1980), but there was no successful regeneration. Gusev et al (1986) were able to regenerate tobacco shoots, which contained cyanobacteria in their intercellular spaces, but they did not root the regenerates. Azospirilla have also been cultured together with plant tissues.…”

Section: Introductionmentioning

confidence: 95%

In vitro establishment of nitrogen-fixing strawberry (Fragaria × ananassa) via artificial symbiosis with Azomonas insignis

Preininger

Zatykó²,

Szűcs

et al. 1997

In Vitro Cell.Dev.Biol.-Plant

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Artificial symbiosis was established between diazotrophic Azomonas insignia and strawbeH'y (Fragaria × ananassa). The partnership was created by in vitro techniques through callus induction and organogenesis. Suitable micropropagation [M3 = Murashige and Skoog (1962) (MS) basal medium supplemented with 2.5 btM N"-benzyladenine (BA), 0.3 gM gibberellic acid (GA3) , 2.2 gM indole-3-butyric acid (IBA), and 3% sucrose] and plant regeneration [R3 = MS mineral salts + 555 gM myo-inositol, 1.2 I.tM thiamine HC1, 4.4 gM BA, 0.5 gM IBA, 0.3 gM c~-naphthaleneacetic acid (NAA), 0.5 gM 2,4-dichlorophenoxyacetic acid (2,4-D)] media were developed for the test cultivar Fert(;di F5. New shoots containing bacteria were rooted, acclimatized, and planted outdoors. The basis of the partnership during the in vitro phase is the bacterial dependence on the plant metabolic activity, using maltose in the medium as carbon and energy source that can be utilized by the plant cells only. The presence of bacteria in the intercellular spaces of the callus tissues and regenerated plants was proved by re-isolation and microscopic techniques. Nitrogenase activity was also detected in the plant tissues.

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“…The creation of novel symbioses with Ngfixing microorganisms may provide an alternative method to the introduction of isolated nif genes into plants. To date, novel associations between Rhizobium and rice (Al-Mallah, Davey & Cocking, 1989) and between Anabaena and tobacco (Gusev et al, 1986) have been reported. The former association was obtained by treating rice roots with cell wall degrading enzymes followed by inoculation with rhizobia.…”

Section: Introductionmentioning

confidence: 99%

Colonization of wheat Triticum vulgare L.) by N₂‐fixing cyanobacteria: I. A survey of soil cyanobacterial isolates forming associations with roots

et al. 1991

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SUMMARYA variety of heterocystous, Nj-fixing cyanobacteria, isolated from soils, were identified as members of the genera Nostoc, Anabaena and Cylindrospermum. These isolates were tested for their ability to fortn associations with the roots of wheat seedlings grown in liquid culture. Two types of associations were recognized: loose associations with cyanobacteria] filaments growing between root hairs, which were typical of the Anabaena isolates, and tight associations of microcolonies in intimate association with the root surface, which were restricted to certain Nostoc isolates. Differences in nitrogenase activities of the free-living and associated cyanobacteria, together with the effects of added nitrate, indicate that nitrogenase activity may be influenced by the plant and/or its products.

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Introduction of a nitrogen-fixing cyanobacterium into tobacco shoot regenerates

Cited by 25 publications

References 24 publications

Physiological characterization and electron microscopic investigation of cyanobacteria associated with wheat rhizosphere

Physiological characterization and electron microscopic investigation of cyanobacteria associated with wheat rhizosphere

In vitro establishment of nitrogen-fixing strawberry (Fragaria × ananassa) via artificial symbiosis with Azomonas insignis

Colonization of wheat Triticum vulgare L.) by N₂‐fixing cyanobacteria: I. A survey of soil cyanobacterial isolates forming associations with roots

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Introduction of a nitrogen-fixing cyanobacterium into tobacco shoot regenerates

Cited by 25 publications

References 24 publications

Physiological characterization and electron microscopic investigation of cyanobacteria associated with wheat rhizosphere

Physiological characterization and electron microscopic investigation of cyanobacteria associated with wheat rhizosphere

In vitro establishment of nitrogen-fixing strawberry (Fragaria × ananassa) via artificial symbiosis with Azomonas insignis

Colonization of wheat Triticum vulgare L.) by N2‐fixing cyanobacteria: I. A survey of soil cyanobacterial isolates forming associations with roots

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Colonization of wheat Triticum vulgare L.) by N₂‐fixing cyanobacteria: I. A survey of soil cyanobacterial isolates forming associations with roots