Introduction: From the Orient as Theater to the Orient in the Theater

Pao, Angela C.

doi:10.9783/9781512806809-002

Cited by 2 publications

(2 citation statements)

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“…Both NS motifs are two stretches of conserved amino acids that appear in all members of the NNP family [28,29]. Between the TM2 and TM3 domains, a consensus sequence G-x-x-x-D-x-x-G-x-R highly conserved in members of the MFS has been identified [28,30] and in the TM8 domain, another consensus sequence of MFS members is also found [29]. In addition, ZosmaNRT2 contains a conserved motif (F-G-M-R-G-R-L-W) present in NRT2 proteins form photosynthetic eukaryotic organisms [31].…”

Section: Resultsmentioning

confidence: 99%

Molecular Characterization of ZosmaNRT2, the Putative Sodium Dependent High-Affinity Nitrate Transporter of Zostera marina L.

Rubio

Díaz‐García

Amorim‐Silva

et al. 2019

IJMS

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One of the most important adaptations of seagrasses during sea colonization was the capacity to grow at the low micromolar nitrate concentrations present in the sea. In contrast to terrestrial plants that use H+ symporters for high-affinity NO3− uptake, seagrasses such as Zostera marina L. use a Na+-dependent high-affinity nitrate transporter. Interestingly, in the Z. marina genome, only one gene (Zosma70g00300.1; NRT2.1) is annotated to this function. Analysis of this sequence predicts the presence of 12 transmembrane domains, including the MFS domains of the NNP transporter family and the “nitrate signature” that appears in all members of the NNP family. Phylogenetic analysis shows that this sequence is more related to NRT2.5 than to NRT2.1, sharing a common ancestor with both monocot and dicot plants. Heterologous expression of ZosmaNRT2-GFP together with the high-affinity nitrate transporter accessory protein ZosmaNAR2 (Zosma63g00220.1) in Nicotiana benthamiana leaves displayed four-fold higher fluorescence intensity than single expression of ZosmaNRT2-GFP suggesting the stabilization of NRT2 by NAR2. ZosmaNRT2-GFP signal was present on the Hechtian-strands in the plasmolyzed cells, pointing that ZosmaNRT2 is localized on the plasma membrane and that would be stabilized by ZosmaNAR2. Taken together, these results suggest that Zosma70g00300.1 would encode a high-affinity nitrate transporter located at the plasma membrane, equivalent to NRT2.5 transporters. These molecular data, together with our previous electrophysiological results support that ZosmaNRT2 would have evolved to use Na+ as a driving ion, which might be an essential adaptation of seagrasses to colonize marine environments.

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Section: Resultsmentioning

confidence: 99%

Molecular Characterization of ZosmaNRT2, the Putative Sodium Dependent High-Affinity Nitrate Transporter of Zostera marina L.

Rubio

Díaz‐García

Amorim‐Silva

et al. 2019

IJMS

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“…Interestingly, the constitutive expression of NAS genes from other plant species caused an increase in Zn 2+ translocation and accumulation in polished rice grains (Masuda et al, 2009; Lee et al, 2011), illustrating the significant importance of the NAS proteins in the Zn 2+ translocation in plants. The major facilitator superfamily (MFS) transporter (Pao et al, 1998), zinc-induced facilitator 1 (ZIF1), was shown to contribute to Zn 2+ tolerance in Arabidopsis (Haydon and Cobbett, 2007), and tonoplast-localized ZIF1 proteins have been implicated in vacuolar Zn 2+ sequestration (Arrivault et al, 2006; Kawachi et al, 2009). Under Zn 2+ deficiency, the vacuole-stored Zn 2+ was remobilized (Lanquar et al, 2004) to the cytosol.…”

Section: Introductionmentioning

confidence: 99%

Interactions Between Phosphorus, Zinc, and Iron Homeostasis in Nonmycorrhizal and Mycorrhizal Plants

Xie

Fan

et al. 2019

Front. Plant Sci.

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Phosphorus (P), zinc (Zn), and iron (Fe) are three essential elements for plant survival, and severe deficiencies in these nutrients lead to growth retardation and crop yield reduction. This review synthesizes recent progress on how plants coordinate the acquisition and signaling of Pi, Zn, and Fe from surrounding environments and which genes are involved in these Pi–Zn–Fe interactions with the aim of better understanding of the cross-talk between these macronutrient and micronutrient homeostasis in plants. In addition, identification of genes important for interactions between Pi, Zn, and/or Fe transport and signaling is a useful target for breeders for improvement in plant nutrient acquisition. Furthermore, to understand these processes in arbuscular mycorrhizal plants, the preliminary examination of interactions between Pi, Zn, and Fe homeostasis in some relevant crop species has been performed at the physiological level and is summarized in this article. In conclusion, the development of integrative study of cross-talks between Pi, Zn, and Fe signaling pathway in mycorrhizal plants will be essential for sustainable agriculture all around the world.

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Introduction: From the Orient as Theater to the Orient in the Theater

Cited by 2 publications

References 0 publications

Molecular Characterization of ZosmaNRT2, the Putative Sodium Dependent High-Affinity Nitrate Transporter of Zostera marina L.

Molecular Characterization of ZosmaNRT2, the Putative Sodium Dependent High-Affinity Nitrate Transporter of Zostera marina L.

Interactions Between Phosphorus, Zinc, and Iron Homeostasis in Nonmycorrhizal and Mycorrhizal Plants

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