Introduction

Dettlaff, T. A.; Ginsburg, Anna S.; Schmalhausen, Olga I.

doi:10.1007/978-3-642-77057-9_1

Cited by 13 publications

(8 citation statements)

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“…In order to use the sterlet as a more experimentally tractable model for lateral line organ development than the Mississippi paddlefish ( Modrell et al, 2011a ; Modrell et al, 2011b ; Modrell et al, 2017a ; Modrell et al, 2017b ), we first characterized the timing and distribution of lateral line hair cell and electroreceptor differentiation (staging according to Dettlaff et al, 1993 ). The development of lateral line placodes, neuromasts and ampullary organs had previously been described in the shovelnose sturgeon, Scaphirhynchus platorynchus ( Gibbs and Northcutt, 2004a ); ampullary organ formation had also been described in a sturgeon in the same genus as the sterlet, the Adriatic sturgeon, A. naccari ( Camacho et al, 2007 ).…”

Section: Resultsmentioning

confidence: 99%

“…Sterlet animal husbandry, in vitro fertilization and the rearing of embryos and yolk-sac larvae are described in detail in Stundl et al (2022) . Sterlet embryos were staged according to Dettlaff et al (1993) . Animal care was approved by the Ministry of Agriculture of the Czech Republic (MSMT-12550/2016-3), followed the principles of the European Union Harmonized Animal Welfare Act of the Czech Republic, and Principles of Laboratory Animal Care and National Laws 246/1992 “Animal Welfare”, and was conducted in accordance with the Animal Research Committee of RIFCH.…”

Section: Methodsmentioning

confidence: 99%

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Identification of multiple transcription factor genes potentially involved in the development of electrosensory versus mechanosensory lateral line organs

Minařík,

Modrell,

Gillis

et al. 2024

Front. Cell Dev. Biol.

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In electroreceptive jawed vertebrates, embryonic lateral line placodes give rise to electrosensory ampullary organs as well as mechanosensory neuromasts. Previous reports of shared gene expression suggest that conserved mechanisms underlie electroreceptor and mechanosensory hair cell development and that electroreceptors evolved as a transcriptionally related “sister cell type” to hair cells. We previously identified only one transcription factor gene, Neurod4, as ampullary organ-restricted in the developing lateral line system of a chondrostean ray-finned fish, the Mississippi paddlefish (Polyodon spathula). The other 16 transcription factor genes we previously validated in paddlefish were expressed in both ampullary organs and neuromasts. Here, we used our published lateral line organ-enriched gene-set (arising from differential bulk RNA-seq in late-larval paddlefish), together with a candidate gene approach, to identify 25 transcription factor genes expressed in the developing lateral line system of a more experimentally tractable chondrostean, the sterlet (Acipenser ruthenus, a small sturgeon), and/or that of paddlefish. Thirteen are expressed in both ampullary organs and neuromasts, consistent with conservation of molecular mechanisms. Seven are electrosensory-restricted on the head (Irx5, Irx3, Insm1, Sp5, Satb2, Mafa and Rorc), and five are the first-reported mechanosensory-restricted transcription factor genes (Foxg1, Sox8, Isl1, Hmx2 and Rorb). However, as previously reported, Sox8 is expressed in ampullary organs as well as neuromasts in a catshark (Scyliorhinus canicula), suggesting the existence of lineage-specific differences between cartilaginous and ray-finned fishes. Overall, our results support the hypothesis that ampullary organs and neuromasts develop via largely conserved transcriptional mechanisms, and identify multiple transcription factors potentially involved in the formation of electrosensory versus mechanosensory lateral line organs.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Identification of multiple transcription factor genes potentially involved in the development of electrosensory versus mechanosensory lateral line organs

Minařík,

Modrell,

Gillis

et al. 2024

Front. Cell Dev. Biol.

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“…Free-swimming larvae were kept in well-oxygenated containers at 15 to 17 °C until desired developmental stage, and they were euthanized using an overdose of tricaine (1 g/L) prior to fixation in 4% PFA. Sterlet development was staged as previously described by Dettlaff et al ( 52 ). Senegal bichirs ( Polypterus senegalus , Cuvier 1829) were obtained from the Department of Zoology, Faculty of Science, Charles University in Prague, the Czech Republic, and spotted gars ( Lepisosteus oculatus , Winchell 1864) from the Department of Integrative Biology, Michigan State University, MI, USA.…”

Section: Methodsmentioning

confidence: 99%

Ancient vertebrate dermal armor evolved from trunk neural crest

Štundl

Martik

Chen

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

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Bone is an evolutionary novelty of vertebrates, likely to have first emerged as part of ancestral dermal armor that consisted of osteogenic and odontogenic components. Whether these early vertebrate structures arose from mesoderm or neural crest cells has been a matter of considerable debate. To examine the developmental origin of the bony part of the dermal armor, we have performed in vivo lineage tracing in the sterlet sturgeon, a representative of nonteleost ray-finned fish that has retained an extensive postcranial dermal skeleton. The results definitively show that sterlet trunk neural crest cells give rise to osteoblasts of the scutes. Transcriptional profiling further reveals neural crest gene signature in sterlet scutes as well as bichir scales. Finally, histological and microCT analyses of ray-finned fish dermal armor show that their scales and scutes are formed by bone, dentin, and hypermineralized covering tissues, in various combinations, that resemble those of the first armored vertebrates. Taken together, our results support a primitive skeletogenic role for the neural crest along the entire body axis, that was later progressively restricted to the cranial region during vertebrate evolution. Thus, the neural crest was a crucial evolutionary innovation driving the origin and diversification of dermal armor along the entire body axis.

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“…The sterlet sturgeons ( Acipenser ruthenus ) were bred in the Research Institute of Fish Culture and Hydrobiology in Vodnany, University of South Bohemia. Embryos were staged [63,64] and fixed in methanol at −20°C as described [16].…”

Section: Methodsmentioning

confidence: 99%

Pre-mandibular pharyngeal pouches in early non-teleost fish embryos

Horackova,

Pospisilova,

Stundl

et al. 2023

Proc. R. Soc. B.

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The vertebrate pharynx is a key embryonic structure with crucial importance for the metameric organization of the head and face. The pharynx is primarily built upon progressive formation of paired pharyngeal pouches that typically develop in post-oral (mandibular, hyoid and branchial) domains. However, in the early embryos of non-teleost fishes, we have previously identified pharyngeal pouch-like outpocketings also in the pre-oral domain of the cranial endoderm. This pre-oral gut (POG) forms by early pouching of the primitive gut cavity, followed by the sequential formation of typical (post-oral) pharyngeal pouches. Here, we tested the pharyngeal nature of the POG by analysing expression patterns of selected core pharyngeal regulatory network genes in bichir and sturgeon embryos. Our comparison revealed generally shared expression patterns, including Shh, Pax9 , Tbx1, Eya1 , Six1 , Ripply3 or Fgf8, between early POG and post-oral pharyngeal pouches . POG thus shares pharyngeal pouch-like morphogenesis and a gene expression profile with pharyngeal pouches and can be regarded as a pre-mandibular pharyngeal pouch. We further suggest that pre-mandibular pharyngeal pouches represent a plesiomorphic vertebrate trait inherited from our ancestor's pharyngeal metameric organization, which is incorporated in the early formation of the pre-chordal plate of vertebrate embryos.

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Introduction

Cited by 13 publications

References 0 publications

Identification of multiple transcription factor genes potentially involved in the development of electrosensory versus mechanosensory lateral line organs

Identification of multiple transcription factor genes potentially involved in the development of electrosensory versus mechanosensory lateral line organs

Ancient vertebrate dermal armor evolved from trunk neural crest

Pre-mandibular pharyngeal pouches in early non-teleost fish embryos

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