Introducing the Newly Isolated Bacterium Aneurinibacillus sp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers–1. Isolation and Characterization of the Bacterium

Pernicová, Iva; Nováčková, Ivana; Sedláček, Petr; Kouřilová, Xenie; Kalina, Michal; Kovalcik, Adriána; Koller, Martin; Nebesářová, Jana; Krzyžánek, Vladislav; Hrubanová, Kamila; Másilko, Jiří; Slaninová, Eva; Obruča, Stanislav

doi:10.3390/polym12061235

Cited by 29 publications

(16 citation statements)

References 44 publications

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“…However, several limitations currently exist when PHB is produced at industrial scale capacities. The high costs of production, low yield, susceptibility to degradation, technology complexities including extraction difficulties, are among the challenges facing PHB production [2]. In order to reduce the costs of production and achieve economic viability in competition with the current low petroleum plastics manufacturing costs, a series of new emerging bacterial fermentation processes are being evaluated [3], which have the potentially to accentuate the kinetics of microbial growth and PHB accumulation to the levels necessary for profitable process engineering and production serving the plastics value chain stakeholders.…”

Section: Introductionmentioning

confidence: 99%

Production of Polyhydroxybutyrate (PHB) and Factors Impacting Its Chemical and Mechanical Characteristics

et al. 2020

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Plastic pollution is fueling the grave environmental threats currently facing humans, the animal kingdom, and the planet. The pursuit of renewable resourced biodegradable materials commenced in the 1970s with the need for carbon neutral fully sustainable products driving important progress in recent years. The development of bioplastic materials is highlighted as imperative to the solutions to our global environment challenges and to the restoration of the wellbeing of our planet. Bio-based plastics are becoming increasingly sustainable and are expected to substitute fossil-based plastics. Bioplastics currently include both, nondegradable and biodegradable compositions, depending on factors including the origins of production and post-use management and conditions. Among the most promising materials being developed and evaluated is polyhydroxybutyrate (PHB), a microbial bioprocessed polyester belonging to the polyhydroxyalkanoate (PHA) family. This biocompatible and non-toxic polymer is biosynthesized and accumulated by a number of specialized bacterial strains. The favorable mechanical properties and amenability to biodegradation when exposed to certain active biological environments, earmark PHB as a high potential replacement for petrochemical based polymers such as ubiquitous high density polyethylene (HDPE). To date, high production costs, minimal yields, production technology complexities, and difficulties relating to downstream processing are limiting factors for its progression and expansion in the marketplace. This review examines the chemical, mechanical, thermal, and crystalline characteristics of PHB, as well as various fermentation processing factors which influence the properties of PHB materials.

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Section: Introductionmentioning

confidence: 99%

Production of Polyhydroxybutyrate (PHB) and Factors Impacting Its Chemical and Mechanical Characteristics

et al. 2020

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“…H1 (see Refs. [ 3 , 12 ]) to adjust selected product properties via different cultivation strategies. Compared to P3HB production by C. necator , the productivity of the copolymer synthesis is considerably lower.…”

Section: Discussionmentioning

confidence: 99%

“…Fortunately, a thermophilic bacterial strain, Aneurinibacillus sp. H1 has appeared as this kind of optimal producent if cultivation conditions are controlled [ 3 ]. Aneurinibacillus is a remarkable producent of poly(3-hydroxybutyrate- co -4-hydroxybutyrate) copolymer able to incorporate 4-hydroxybutyrate (4HB) units up to 90 mol.% by utilising 1,4-butanediol as a precursor, while the monomer ratio in the final copolymer is mirrored to carbon sources (precursor) ratio in the medium [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

“…Aneurinibacillus is a remarkable producent of poly(3-hydroxybutyrate- co -4-hydroxybutyrate) copolymer able to incorporate 4-hydroxybutyrate (4HB) units up to 90 mol.% by utilising 1,4-butanediol as a precursor, while the monomer ratio in the final copolymer is mirrored to carbon sources (precursor) ratio in the medium [ 3 ]. In addition, this thermophilic bacterial strain is also able to produce terpolymers consisting of 3-hydroxybutyrate, 4-hydroxybutyrate, and 3-hydroxyvalerate and does not produce toxic endotoxins [ 3 ]. These copolymers seem to be suitable polymers for thin-film production due to their favourable material properties for different applications [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Differing Monomer Compositions on Properties of P(3HB-co-4HB) Synthesized by Aneurinibacillus sp. H1 for Various Applications

et al. 2022

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Films prepared from poly(3-hydroxybutyrate-co-4-hydroxybutyrate) copolymers produced by Aneurinibacillus sp. H1 using an automatic film applicator were homogeneous and had a defined thickness, which allowed a detailed study of physicochemical properties. Their properties were compared with those of a poly (3-hydroxybutyrate) homopolymer film prepared by the same procedure, which proved to be significantly more crystalline by DSC and XRD. Structural differences between samples had a major impact on their properties. With increasing 4-hydroxybutyrate content, the ductility and release rate of the model hydrophilic active ingredient increased significantly. Other observed properties, such as the release of the hydrophobic active substance, the contact angle with water and ethylene glycol, or the surface morphology and roughness, were also affected by the composition. The identified properties predetermine these copolymers for wide use in areas such as biomedicine or smart biodegradable packaging for food or cosmetics. The big advantage is the possibility of fine-tuning properties simply by changing the fermentation conditions.

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“…There are also some described PHA producers in this group, including Chelatococcus thermostellatus [22], Caldimonas taiwanensis [23], or new isolate bacteria Aneurinibacillus sp. H1 [24]. PHA production has also recently been described in the bacterium Schelegelella thermodepolymerans using xylose as the most preferred substrate [25].…”

Section: Introductionmentioning

confidence: 99%

Biotechnological Conversion of Grape Pomace to Poly(3-hydroxybutyrate) by Moderately Thermophilic Bacterium Tepidimonas taiwanensis

et al. 2021

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Polyhydroxyalkanoates (PHA) are microbial polyesters that have recently come to the forefront of interest due to their biodegradability and production from renewable sources. A potential increase in competitiveness of PHA production process comes with a combination of the use of thermophilic bacteria with the mutual use of waste substrates. In this work, the thermophilic bacterium Tepidimonas taiwanensis LMG 22826 was identified as a promising PHA producer. The ability to produce PHA in T. taiwanensis was studied both on genotype and phenotype levels. The gene encoding the Class I PHA synthase, a crucial enzyme in PHA synthesis, was detected both by genome database search and by PCR. The microbial culture of T. taiwanensis was capable of efficient utilization of glucose and fructose. When cultivated on glucose as the only carbon source at 50°C, the PHA titers reached up to 3.55 g/L, and PHA content in cell dry mass was 65%. The preference of fructose and glucose opens the possibility to employ T. taiwanensis for PHA production on various food wastes rich in these abundant sugars. In this work, PHA production on grape pomace extracts was successfully tested.

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Introducing the Newly Isolated Bacterium Aneurinibacillus sp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers–1. Isolation and Characterization of the Bacterium

Cited by 29 publications

References 44 publications

Production of Polyhydroxybutyrate (PHB) and Factors Impacting Its Chemical and Mechanical Characteristics

Production of Polyhydroxybutyrate (PHB) and Factors Impacting Its Chemical and Mechanical Characteristics

Effects of Differing Monomer Compositions on Properties of P(3HB-co-4HB) Synthesized by Aneurinibacillus sp. H1 for Various Applications

Biotechnological Conversion of Grape Pomace to Poly(3-hydroxybutyrate) by Moderately Thermophilic Bacterium Tepidimonas taiwanensis

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