Introducing SELEX via a semester‐long course‐based undergraduate research experience (CURE)

Abstract

With the growing importance of the field of RNA biology, undergraduates need to perform RNA-related research. Systematic evolution of ligands by exponential enrichment (SELEX) has become an important method in RNA biology. The principles of SELEX were applied to a semester-long course-based undergraduate research experience (CURE) in which two rounds of in vivo functional selection of regions of a viral RNA were performed. As the labwork had an unknown outcome, students indicated that they were excited by the work and became invested in the experience. By completing two rounds of SELEX, the students repeated molecular methods (e.g., RNA extraction, RT-PCR, agarose gel electrophoresis, DNA purification, cloning, and sequence analysis) and reported that repetition reinforced their learning and helped them build confidence in their lab abilities. Students also appreciated that they did not learn a "technique-per-week" without context, but rather they understood why certain methods were used for certain molecular tasks. Results from a 19-question multiple-choice assessment indicated increased comprehension of theory underlying methods performed. Details regarding experimental methods and timeline, and assessment and attitudinal results from three student cohorts, are described herein.

Knowledge-Map Analysis of Undergraduate Biochemistry Teaching Research: A Bibliometric Study from 2012 to 2021

“…Unlike apprenticeship research experiences conducted in a lab with limited participants, CUREs are designed for large classes as core learning activities that allow as many students as possible to be exposed to scientific research experiences . Studies have demonstrated that the CUREs positively affect students’ self-efficacy, self-determination, and problem-solving abilities as well as build their confidence in lab abilities . In addition, early engagement of undergraduates in CUREs has positive effects on graduation rates and the completion of science degrees .…”

“…5 Studies have demonstrated that the CUREs positively affect students' self-efficacy, self-determination, and problem-solving abilities 71 as well as build their confidence in lab abilities. 85 In addition, early engagement of undergraduates in CUREs has positive effects on graduation rates and the completion of science degrees. 86 Therefore, the CUREs may represent a promising solution for the improved learning outcomes of undergraduate biochemistry courses, although several obstacles, including faulty competency, equipment, and techni-ques, need to be carefully evaluated when applying CUREs in undergraduate institutions.…”