Introducing online multicolumn two-dimensional liquid chromatography screening for facile selection of stationary and mobile phase conditions in both dimensions

Wang, Heather; Lhotka, Hayley R.; Bennett, Raffeal; Potapenko, Miraslava; Pickens, Chad J.; Mann, Benjamin F.; Ahmad, Imad A. Haidar; Regalado, Erik L.

doi:10.1016/j.chroma.2020.460895

Cited by 39 publications

(30 citation statements)

References 48 publications

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“…Recently, Regalado and co‐workers discussed the addition of multiple selector valves to a conventional 2D‐LC‐MS system. This approach enables screening of multiple column and mobile phase chemistries in both separation dimensions in a completely automated fashion, allowing for rapid screening of different column/eluent combination without user intervention 170 . Simplified method development was successfully demonstrated for multicomponent mixtures of drug substances, the separation of which was challenging because ingredients were closely related (as is often the case with API‐related impurities) 126,171 .…”

Section: Recent Key Applicationsmentioning

confidence: 99%

Advances in ultra‐high‐pressure and multi‐dimensional liquid chromatography instrumentation and workflows

Vos

Stoll

Buckenmaier

et al. 2021

Analytical Science Advances

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The present contribution discusses recent advances in ultra‐high‐pressure liquid chromatography (UHPLC) and multi‐dimensional liquid chromatography (MDLC) technology. First, new developments in UHPLC column technology and system design are highlighted. The latter includes a description of a novel injector concept enabling method speed‐up, emerging detectors, and instrument diagnostics approaches. Next, online MDLC workflows are reviewed and advances in modulation technology are highlighted. Finally, key applications published in 2020 are reviewed.

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Section: Recent Key Applicationsmentioning

confidence: 99%

Advances in ultra‐high‐pressure and multi‐dimensional liquid chromatography instrumentation and workflows

Vos

Stoll

Buckenmaier

et al. 2021

Analytical Science Advances

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“…This instrument was used for separation of stereoisomers prepared by coupling of racemic ibuprofen and racemic 1‐phenylethylamine using peptide coupling reagent. The automated multicolumn and multieluent screenings are essential methodology in the pharmaceutical industry 108,109 . SFC has quickly evolved to become the preferred method for the enantiomeric analysis in pharmaceutical research and development.…”

Section: Other Studiesmentioning

confidence: 99%

Macrocyclic glycopeptide‐based chiral selectors for enantioseparation in sub/supercritical fluid chromatography

Folprechtová

Kalíková

2020

Analytical Science Advances

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Increasing number of reported works dealing with macrocyclic glycopeptide‐based columns in sub/supercritical fluid chromatography (SFC) points to the growing interest in this area. With the development and production of sub 2 µm fully porous particles and superficially porous particles with bonded macrocyclic glycopeptides, significant improvements have been made in ultrafast high efficiency chiral SFC. This review article gives an overview of macrocyclic glycopeptide‐based chiral selectors that were used in theoretical studies and/or applications in SFC. The review covers the period from 1997 when macrocyclic glycopeptides were first used in SFC till the end of July 2020 according to Web of Science. This work can also be helpful to analysts searching for an appropriate method for the separation/determination of enantiomers of their interest.

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“…Other orthogonal separation systems, including 2D-NPLC × RPLC [16], 2D-SEC × RPLC [17][18][19], 2D-chiral × chiral [20], and 2D-achiral × chiral [21], temperatureresponsive LC × RPLC [22], countercurrent chromatography × RPLC [23], IEC × assay-based RPLC [24], SFC × RPLC [25], IPC × RPLC [26], and IEC × SEC [27] have also been developed and applied toward the analysis of biosamples. Moreover, advanced strategies [28][29][30] were proposed for the development of a more systematic, less tedious, and less reliant on user expertise 2D-LC method. Notably, the introduction of a new stationary phase with unique selectivity has become a popular strategy for the construction of new 2D-LC systems [9,31].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive profiling of Sanguisorba officinalis using offline two‐dimensional mixed‐mode liquid chromatography × reversed‐phase liquid chromatography, tandem high‐resolution mass spectrometry, and molecular network

Dai

Zhang

Xiong

et al. 2022

J of Separation Science

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The profiling of natural products is important in modern biological sciences and new drug development. However, the separation and characterization of complex herbal extracts are significantly challenging for researchers in the biochemical field. Herein, an offline two-dimensional mixed-mode liquid chromatography × reversed-phase liquid chromatography system is developed. Our system exhibits high orthogonality and is composed of a newly prepared stationary phase in the first dimension and a traditional C 18 phase in the second dimension, and is operated in combination with a high-resolution mass spectrometry and molecular network. Sanguisorba officinalis L. is studied using the proposed method owing to its bioactivity. With the aid of orthogonal separation, the ionization of the individual components is improved. The number of detected compounds and separated peaks are significantly increased when one-dimensional liquid chromatography is upgraded to two-dimensional liquid chromatography.In addition, 270 compounds (127 of which are tentatively characterized as new compounds, and further confirmation is needed) are successfully characterized based on their fragmentation patterns under the guidance of molecular network, while only 95 compounds are characterized using one-dimensional liquid chromatography and high-resolution mass spectrometry. The results indicate that the developed offline two-dimensional mixed-mode liquid chromatography ×

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Introducing online multicolumn two-dimensional liquid chromatography screening for facile selection of stationary and mobile phase conditions in both dimensions

Cited by 39 publications

References 48 publications

Advances in ultra‐high‐pressure and multi‐dimensional liquid chromatography instrumentation and workflows

Advances in ultra‐high‐pressure and multi‐dimensional liquid chromatography instrumentation and workflows

Macrocyclic glycopeptide‐based chiral selectors for enantioseparation in sub/supercritical fluid chromatography

Comprehensive profiling of Sanguisorba officinalis using offline two‐dimensional mixed‐mode liquid chromatography × reversed‐phase liquid chromatography, tandem high‐resolution mass spectrometry, and molecular network

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