Introducing MINA – The Molecularly Imprinted Nanoparticle Assay

Shutov, Roman V.; Guerreiro, António; Moczko, Ewa; Vargas-Sansalvador, Isabel Perez de; Chianella, Iva; Whitcombe, Michael J.; Piletsky, Sergey A.

doi:10.1002/smll.201301996

Cited by 39 publications

(21 citation statements)

References 16 publications

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“…High affinity material is produced by virtue of an in-built affinity separation step, the template may be re-used, and the surface chemistry can be modified chemically without affecting the recognition properties of the MIPs56. The resultant particles are suitable for applications where antibodies are traditionally used, including sensors22232425 and assays71626. Imprinted nanoparticles have also been shown to selectively detect mammalian cell types by their expression of antigens2728.…”

Section: Resultsmentioning

confidence: 99%

A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format

Smolińska-Kempisty

Guerreiro

Canfarotta

et al. 2016

Sci Rep

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100

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Here we show that molecularly imprinted polymer nanoparticles, prepared in aqueous media by solid phase synthesis with immobilised L-thyroxine, glucosamine, fumonisin B2 or biotin as template, can demonstrate comparable or better performance to commercially produced antibodies in enzyme-linked competitive assays. Imprinted nanoparticles-based assays showed detection limits in the pM range and polymer-coated microplates are stable to storage at room temperature for at least 1 month. No response to analyte was detected in control experiments with nanoparticles imprinted with an unrelated template (trypsin) but prepared with the same polymer composition. The ease of preparation, high affinity of solid-phase synthesised imprinted nanoparticles and the lack of requirement for cold chain logistics make them an attractive alternative to traditional antibodies for use in immunoassays.

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Section: Resultsmentioning

confidence: 99%

A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format

Smolińska-Kempisty

Guerreiro

Canfarotta

et al. 2016

Sci Rep

Self Cite

100

View full text Add to dashboard Cite

show abstract

“…Moreover, we foresee the potential to further extend the method by a direct integration of the MIPs on the target and to include multi‐functional MIPs, e.g. able of recognition and catalysis (Wulff et al ., ; Lakshmi et al ., ; Shutov et al ., ), with the aim of developing the “lab‐on‐a plate” MALDI target (Urban et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Molecularly imprinted polymers coupled to matrix assisted laser desorption ionization mass spectrometry for femtomoles detection of cardiac troponin I peptides

Cenci

Anesi

Busato

et al. 2015

J of Molecular Recognition

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Molecularly imprinted polymers (MIPs) were combined to MALDI-TOF-MS to evaluate a selective enrichment (SE) method for the determination of clinically relevant biomarkers from complex biological samples. The concept was proven with the myocardial injury marker Troponin I (cTnI). In a first part, MIP materials entailed for the recognition of cTnI epitopes (three peptides selected) were prepared and characterized in dimensions (0.7-2μm), dissociation constants (58-817 nM), kinetics of binding (5-60 min), binding capacity (ca. 1.5 µg/mg polymer), imprinting factors (3 > IF > 5) and selectivity for the peptide epitope. Then, the MIPs, incubated with cTnI peptides and spotted on the target with the DHB matrix, were assayed for the desorption of the peptides in MALDI-TOF-MS. The measured detection limit was ca. 300 femtomols. Finally, the MIP-SE MALDI-TOF-MS was tested for its ability to enrich in the cTnI peptides from a complex sample, mimic of serum (i.e. 81 peptides of digested albumin). The MIP-SE MALDI-TOF-MS successfully enriched in cTnI peptides from the complex sample proving the technique could offer a flexible platform to prepare entailed materials suitable for diagnostic purposes.

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“…One way to circumvent the latter is to use a stable enzyme analogue such as iron oxide. This was demonstrated recently with a totally abiotic ELISA-type assay 10 based on MIP nanoparticles containing a catalytic core of Fe 3 O 4 with peroxidase-mimicking properties. [11][12][13] In this assay, core-shell nanoparticles containing MIP at the surface are used for their combined recognition and signalling function.…”

Section: Introductionmentioning

confidence: 90%

Formation of target-specific binding sites in enzymes: solid-phase molecular imprinting of HRP

et al. 2016

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Here we introduce a new concept for synthesising molecularly imprinted nanoparticles by using proteins as macro-functional monomers. For a proof-of-concept, a model enzyme (HRP) was cross-linked using glutaraldehyde in the presence of glass beads (solid-phase) bearing immobilized templates such as vancomycin and ampicillin. The cross-linking process links together proteins and protein chains, which in the presence of templates leads to the formation of permanent target-specific recognition sites without adverse effects on the enzymatic activity. Unlike complex protein engineering approaches commonly employed to generate affinity proteins, the method proposed can be used to produce protein-based ligands in a short time period using native protein molecules. These affinity materials are potentially useful tools especially for assays since they combine the catalytic properties of enzymes (for signaling) and molecular recognition properties of antibodies. We demonstrate this concept in an ELISA-format assay where HRP imprinted with vancomycin and ampicillin replaced traditional enzyme-antibody conjugates for selective detection of templates at micromolar concentrations. This approach can potentially provide a fast alternative to raising antibodies for targets that do not require high assay sensitivities; it can also find uses as a biochemical research tool, as a possible replacement for immunoperoxidase-conjugates.

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Introducing MINA – The Molecularly Imprinted Nanoparticle Assay

Cited by 39 publications

References 16 publications

A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format

A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format

Molecularly imprinted polymers coupled to matrix assisted laser desorption ionization mass spectrometry for femtomoles detection of cardiac troponin I peptides

Formation of target-specific binding sites in enzymes: solid-phase molecular imprinting of HRP

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