Introducing initial material and producing sterile form of sugar sorghum (Sorghum saccharatum (L.) Pers.)

Abstract

Introduction. Considering the importance of production of alternative types of biofuel that can be obtained from agricultural bioenergy feedstock, it is necessary to develop breeding of energy crops. In the world of breeding practice for acceleration of its productivity use biotechnological methods which allow not only to reproduce and store the materials created by traditional methods, but also to obtain new forms based on the advanced achievements of biotechnology [1,2]. Therefore, the goal of the research was to establish the influence of the size and the weight of sugar sorghum seeds on its sowing properties and to develop introduction of the base line and its sterilization by using different explants. Experiments with seeds were made on standard and generally accepted scientific techniques [3,4].As source material for receiving sterile culture used seeds and internodes of hybrids of sugar sorghum: Medovy, Nectarny, Sylosny 42. Cultivation was carried out in the thermal areas at temperature of 24 ± 2 °C, illumination of 3000-4000 lux, relative humidity of 65-70% and the photoperiod -16 hours.Sterilization of explants was carried out by dichloride solution of mercury (mercuric chloride), aqueous solution of sodium hypochlorite, chloramines at various concentration and expositions.Efficiency of sterilization defined: for 5-10 days percent of sterile explants, and for 10-14 days -viable.Materials and tools, ware and nutrient mediums prepared according to the standard techniques [ 5, 6].For further cloning of bines used modified solid nutrient medium by prescription of Murasihe and Skuga [7,8].The resulting digital material was processed according to conventional methods [8].Results and discussion. The main condition for a successful receiving of the base line is the sterilization of plant objects consisting in killing of the fungal and bacterial spores on the external surface without damage of internal tissues. For this purpose use various sterilizing agents.From the literature it is known that for superficial sterilization of plant tissues using compounds containing active chlorine (so-