Introducing charge tag <i>via</i> click reaction in living cells for single cell mass spectrometry

Zhuang, Meihui; Hou, Zhuanghao; Chen, Peiyao; Liang, Gaolin; Huang, Guangming

doi:10.1039/d0sc00259c

Cited by 25 publications

(16 citation statements)

References 31 publications

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“…As an unlabeled molecular imaging method, AMSI technique can obtain spatial distribution information of many small-molecule metabolites in a single experiment with little or without any pretreatment. For the imaging of labile metabolites in ambient conditions, the labile group could be protected via in situ chemical derivatization [116].…”

Section: Metabolitesmentioning

confidence: 99%

Recent Advances of Ambient Mass Spectrometry Imaging and Its Applications in Lipid and Metabolite Analysis

Liu

et al. 2021

Metabolites

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Ambient mass spectrometry imaging (AMSI) has attracted much attention in recent years. As a kind of unlabeled molecular imaging technique, AMSI can enable in situ visualization of a large number of compounds in biological tissue sections in ambient conditions. In this review, the developments of various AMSI techniques are discussed according to one-step and two-step ionization strategies. In addition, recent applications of AMSI for lipid and metabolite analysis (from 2016 to 2021) in disease diagnosis, animal model research, plant science, drug metabolism and toxicology research, etc., are summarized. Finally, further perspectives of AMSI in spatial resolution, sensitivity, quantitative ability, convenience and software development are proposed.

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Section: Metabolitesmentioning

confidence: 99%

Recent Advances of Ambient Mass Spectrometry Imaging and Its Applications in Lipid and Metabolite Analysis

Liu

et al. 2021

Metabolites

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“…Single-cell metabolite analysis is a promising method to reveal cellular heterogeneity at the most basic and subtle small molecule level, 1–5 especially living single-cell metabolite analysis, 6 enabling the gathering of the most veritable bio-chemical information and reflecting the cell status with higher fidelity, including cell differentiation and division, 7–9 communication, 1,10 interaction with the environment, 11–13 and stress response. 14 This diverse information is of great significance for the development of cell biology and medicine and facilitates cancer diagnosis, prognosis, and treatment.…”

Section: Introductionmentioning

confidence: 99%

Intact living-cell electrolaunching ionization mass spectrometry for single-cell metabolomics

et al. 2022

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“…In the past few years, single-cell analysis has been used to unravel cellular heterogeneity. − Single-cell mass spectrometry (SCMS), which plays a key role on single-cell analysis, has achieved great progress as well. − A number of SCMS techniques have been developed to distinguish cellular fingerprints, to identify intracellular metabolites, and to discover new biological mechanisms through single-cell metabolomic analysis. − …”

Section: Introductionmentioning

confidence: 99%

Chiral Analysis of Lactate during Direct Contact Coculture by Single-Cell On-Probe Enzymatic Dehydrogenation Derivatization: Unraveling Metabolic Changes Caused by d-Lactate

Zhou

Cao

et al. 2021

Anal. Chem.

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In vitro noncontact cell-based coculture models are frequently employed to study cell-to-cell communication. However, these models cannot accurately represent the complexity of in vivo signaling. D-Lactate is an unusual metabolite produced and released by cancer cells. The characterization of D-lactate is challenging as it shares the same mass but has much lower amounts compared with L-lactate. Herein, D-α-hydroxy acids were specifically recognized and dehydrogenated by D-α-hydroxy acid dehydrogenase. The dehydrogenation products were rapidly quaternized for enhancement of mass signals. An on-probe enzymatic dehydrogenationderivatization method was proposed for chiral analysis of α-hydroxy acids at the single-cell level. It is a promising amplification methodology and affords over 3 orders of magnitude signal enhancement. Furthermore, direct contact coculture models were used to precisely mimic the tumor microenvironment and explore the communication between cancer and normal cells. Single-cell mass spectrometry (SCMS) was further applied to easily sample cell extracts and study the differences of the aspects of small molecule metabolism in cocultured cells. On the basis of direct contact coculture SCMS, several differential small molecule metabolites and differences of oxidative stress between cocultured and monocultured normal cells were successfully detected. Additionally, D-lactate was discovered as a valuable differential metabolite with application of the two developed methods. It may account for the cancer-associated metabolic behavior of normal cells. These changes could be relieved after D-lactate metabolism-related drug treatment. This discovery may promote the investigation of D-lactate metabolism, which may provide a novel direction for cancer therapy.

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Introducing charge tag via click reaction in living cells for single cell mass spectrometry

Abstract:
A charge tag is introduced in living cells via a biocompatible click reaction, which greatly increases the sensitivity for single cell mass spectrometry.

Cited by 25 publications

References 31 publications

Recent Advances of Ambient Mass Spectrometry Imaging and Its Applications in Lipid and Metabolite Analysis

Recent Advances of Ambient Mass Spectrometry Imaging and Its Applications in Lipid and Metabolite Analysis

Intact living-cell electrolaunching ionization mass spectrometry for single-cell metabolomics

Chiral Analysis of Lactate during Direct Contact Coculture by Single-Cell On-Probe Enzymatic Dehydrogenation Derivatization: Unraveling Metabolic Changes Caused by d-Lactate

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Introducing charge tag via click reaction in living cells for single cell mass spectrometry

Abstract: A charge tag is introduced in living cells via a biocompatible click reaction, which greatly increases the sensitivity for single cell mass spectrometry.

Cited by 25 publications

References 31 publications

Recent Advances of Ambient Mass Spectrometry Imaging and Its Applications in Lipid and Metabolite Analysis

Recent Advances of Ambient Mass Spectrometry Imaging and Its Applications in Lipid and Metabolite Analysis

Intact living-cell electrolaunching ionization mass spectrometry for single-cell metabolomics

Chiral Analysis of Lactate during Direct Contact Coculture by Single-Cell On-Probe Enzymatic Dehydrogenation Derivatization: Unraveling Metabolic Changes Caused by d-Lactate

Contact Info

Product

Resources

About

Abstract:
A charge tag is introduced in living cells via a biocompatible click reaction, which greatly increases the sensitivity for single cell mass spectrometry.