Intrinsically disordered protein AtDSS1(V) participates in plant defense response to oxidative stress

Nikolić, Ivana; Nešić, Sofija B; Samardžić, Jelena; Timotijević, Gordana

doi:10.1007/s00709-020-01598-7

Cited by 2 publications

(8 citation statements)

References 28 publications

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“…The exclusive partner of DSS1(V) is the EER5 protein, which is involved in promoting a reduced ethylene response upstream or within the ethylene-activated signaling pathway. Accordingly, promoter analyses revealed the presence of ethylene-responsive elements in the DSS1(V) promoter sequence but not in the DSS1(I) promoter [31].…”

Section: Discussionmentioning

confidence: 90%

“…Our previous study on the role of plant DSS1 proteins indicated that protein and gene expression of plant DSS1 has an altered profile under the influence of different oxidative stressors [31]. Analysis of the Arabidopsis dss1(V) −/− T-DNA insertion mutant line showed increased sensitivity to oxidative stress, suggesting that it may be an important player in the molecular mechanisms of a plant's abiotic stress defense [31]. The Arabidopsis genome contains two copies of the DSS1 gene that differ slightly in length.…”

Section: Discussionmentioning

confidence: 99%

“…Homozygous T-DNA insertional dss1(V) mutant plants were obtained and they showed a 75% reduction in the level of gene expression. Physiologically, the mutants displayed a significant reduction in rosette size and shoot length during growth, as well as a higher sensitivity to oxidative stress [31]. However, since two gene copies, DSS1(I) and DSS1(V), are present in the Arabidopsis genome, single genetic mutants need to be generated for appropriate functional analyses.…”

Section: Crispr/cas9 Based Mutagenesis Of Two Highly Homologous Dss1 ...mentioning

confidence: 99%

“…While sem1 mutants of yeast [29], dss1 mutants of Ustilago maydis [12] and dss-1 mutants of Caenorhabditis elegans [30], have been characterized, information about plant dss1 mutants is insubstantial. Only the T-DNA Atdss1(V) mutant with intron insertion has been characterized so far [31].…”

Section: Introductionmentioning

confidence: 99%

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CRISPR/Cas9-Targeted Disruption of Two Highly Homologous Arabidopsis thaliana DSS1 Genes with Roles in Development and the Oxidative Stress Response

Nikolić

Samardžić

Stevanović

et al. 2023

IJMS

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Global climate change has a detrimental effect on plant growth and health, causing serious losses in agriculture. Investigation of the molecular mechanisms of plant responses to various environmental pressures and the generation of plants tolerant to abiotic stress are imperative to modern plant science. In this paper, we focus on the application of the well-established technology CRISPR/Cas9 genome editing to better understand the functioning of the intrinsically disordered protein DSS1 in plant response to oxidative stress. The Arabidopsis genome contains two highly homologous DSS1 genes, AtDSS1(I) and AtDSS1(V). This study was designed to identify the functional differences between AtDSS1s, focusing on their potential roles in oxidative stress. We generated single dss1(I) and dss1(V) mutant lines of both Arabidopsis DSS1 genes using CRISPR/Cas9 technology. The homozygous mutant lines with large indels (dss1(I)del25 and dss1(V)ins18) were phenotypically characterized during plant development and their sensitivity to oxidative stress was analyzed. The characterization of mutant lines revealed differences in root and stem lengths, and rosette area size. Plants with a disrupted AtDSS1(V) gene exhibited lower survival rates and increased levels of oxidized proteins in comparison to WT plants exposed to oxidative stress induced by hydrogen peroxide. In this work, the dss1 double mutant was not obtained due to embryonic lethality. These results suggest that the DSS1(V) protein could be an important molecular component in plant abiotic stress response.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Crispr/cas9 Based Mutagenesis Of Two Highly Homologous Dss1 ...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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CRISPR/Cas9-Targeted Disruption of Two Highly Homologous Arabidopsis thaliana DSS1 Genes with Roles in Development and the Oxidative Stress Response

Nikolić

Samardžić

Stevanović

et al. 2023

IJMS

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“…In our study, we chose specific genes for further investigation and validation based on their involvement in particular biological processes ( Figure S2 ). Previous research [ 8 , 28 ] highlighted the DSS1(V) gene’s responsiveness to stress as well as its role in defending plant cells against oxidative stress by detoxifying damaged proteins. As a result, we concentrated on processes related to secondary metabolite synthesis, given their significance in plant stress defense.…”

Section: Resultsmentioning

confidence: 99%

Assessing Transcriptomic Responses to Oxidative Stress: Contrasting Wild-Type Arabidopsis Seedlings with dss1(I) and dss1(V) Gene Knockout Mutants

Nikolić,

Milisavljević,

Timotijević

2024

IJMS

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Oxidative stress represents a critical facet of the array of abiotic stresses affecting crop growth and yield. In this paper, we investigated the potential differences in the functions of two highly homologous Arabidopsis DSS1 proteins in terms of maintaining genome integrity and response to oxidative stress. In the context of homologous recombination (HR), it was shown that overexpressing AtDSS1(I) using a functional complementation test increases the resistance of the Δdss1 mutant of Ustilago maydis to genotoxic agents. This indicates its conserved role in DNA repair via HR. To investigate the global transcriptome changes occurring in dss1 plant mutant lines, gene expression analysis was conducted using Illumina RNA sequencing technology. Individual RNA libraries were constructed from three total RNA samples isolated from dss1(I), dss1(V), and wild-type (WT) plants under hydrogen peroxide-induced stress. RNA-Seq data analysis and real-time PCR identification revealed major changes in gene expression between mutant lines and WT, while the dss1(I) and dss1(V) mutant lines exhibited analogous transcription profiles. The Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed significantly enriched metabolic pathways. Notably, genes associated with HR were upregulated in dss1 mutants compared to the WT. Otherwise, genes of the metabolic pathway responsible for the synthesis of secondary metabolites were downregulated in both dss1 mutant lines. These findings highlight the importance of understanding the molecular mechanisms of plant responses to oxidative stress.

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Intrinsically disordered protein AtDSS1(V) participates in plant defense response to oxidative stress

Cited by 2 publications

References 28 publications

CRISPR/Cas9-Targeted Disruption of Two Highly Homologous Arabidopsis thaliana DSS1 Genes with Roles in Development and the Oxidative Stress Response

CRISPR/Cas9-Targeted Disruption of Two Highly Homologous Arabidopsis thaliana DSS1 Genes with Roles in Development and the Oxidative Stress Response

Assessing Transcriptomic Responses to Oxidative Stress: Contrasting Wild-Type Arabidopsis Seedlings with dss1(I) and dss1(V) Gene Knockout Mutants

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