Intrinsically disordered amphiphilic peptides as potential targets in drug delivery vehicles

Vincenzi, Marian; Accardo, Antonella; Costantini, Susan; Scala, Stefania; Portella, Luigi; Trotta, Anna Maria; Ronga, Luisa; Guillon, Jean; Leone, Marilisa; Colonna, Giovanni; Rossi, Filomena; Tesauro, Diego

doi:10.1039/c5mb00358j

“…A CMC of 5 µM is in the typical micro-molar range for peptide amphiphiles. 21,[42][43][44] The peptide's degree of disorder was experimentally verified by measuring the circular dichroism (CD) spectrum of samples of the peptide (unconjugated) and the two IDPA1 variants (Supplementary Figure S2). In addition, the peptide sequence displays a high probability for disorder and the absence of regular secondary structure using the NetSurfP-2.0 bioinformatic algorithm (Supplementary Figure S3).…”

Section: Resultsmentioning

confidence: 99%

Order from Disorder with Intrinsically Disordered Peptide Amphiphiles

Jacoby¹,

Segal²,

Ehm³

et al. 2021

Preprint

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Amphiphilic molecules and their self-assembled structures have long been the target of extensive research due to their potential applications in fields ranging from materials design to biomedical and cosmetic applications. Increasing demands for functional complexity have been met with challenges in biochemical engineering, driving researchers to innovate in the design of new amphiphiles. An emerging class of molecules, namely, peptide amphiphiles, combines key advantages and circumvents some of the disadvantages of conventional phospholipids and block-copolymers. Herein, we present new peptide amphiphiles comprised of an intrinsically disordered peptide conjugated to two variants of hydrophobic dendritic domains. These molecules termed intrinsically disordered peptide amphiphiles (IDPA), exhibit a sharp pH-induced micellar phase-transition from low-dispersity spheres to extremely elongated worm-like micelles. We present an experimental characterization of the transition and propose a theoretical model to describe the pH-response. We also present the potential of the shape transition to serve as a mechanism for the design of a cargo hold-and-release application. Such amphiphilic systems demonstrate the power of tailoring the interactions between disordered peptides for various stimuli-responsive biomedical applications.

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“…A CMC of 5 µM is in the typical micro-molar range for peptide amphiphiles. 21,[42][43][44] The peptide's degree of disorder was experimentally verified by measuring the circular dichroism (CD) spectrum of samples of the peptide (unconjugated) and the two IDPA1 variants (Supplementary Figure S2). In addition, the peptide sequence displays a high probability for disorder and the absence of regular secondary structure using the NetSurfP-2.0 bioinformatic algorithm (Supplementary Figure S3).…”

Section: Resultsmentioning

confidence: 99%

Order from disorder with intrinsically disordered peptide amphiphiles

Jacoby,

Asher,

Ehm

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…A CMC of 5 µM is in the typical micro-molar range for peptide amphiphiles. 21,[42][43][44] The peptide's degree of disorder was experimentally verified by measuring the circular dichroism (CD) spectrum of samples of the peptide (unconjugated) and the two IDPA1 variants (Supplementary Figure S2). In addition, the peptide sequence displays a high probability for disorder and the absence of regular secondary structure using the NetSurfP-2.0 bioinformatic algorithm (Supplementary Figure S3).…”

Section: Resultsmentioning

confidence: 99%

Order from Disorder with Intrinsically Disordered Peptide Amphiphiles

Jacoby¹,

Segal²,

Ehm³

et al. 2021

Preprint

0

View full text Add to dashboard Cite

Amphiphilic molecules and their self-assembled structures have long been the target of extensive research due to their potential applications in fields ranging from materials design to biomedical and cosmetic applications. Increasing demands for functional complexity have been met with challenges in biochemical engineering, driving researchers to innovate in the design of new amphiphiles. An emerging class of molecules, namely, peptide amphiphiles, combines key advantages and circumvents some of the disadvantages of conventional phospholipids and block-copolymers. Herein, we present new peptide amphiphiles comprised of an intrinsically disordered peptide conjugated to two variants of hydrophobic dendritic domains. These molecules termed intrinsically disordered peptide amphiphiles (IDPA), exhibit a sharp pH-induced micellar phase-transition from low-dispersity spheres to extremely elongated worm-like micelles. We present an experimental characterization of the transition and propose a theoretical model to describe the pH-response. We also present the potential of the shape transition to serve as a mechanism for the design of a cargo hold-and-release application. Such amphiphilic systems demonstrate the power of tailoring the interactions between disordered peptides for various stimuli-responsive biomedical applications.

show abstract

Intrinsically disordered amphiphilic peptides as potential targets in drug delivery vehicles

Cited by 9 publications

References 30 publications

Order from Disorder with Intrinsically Disordered Peptide Amphiphiles

Order from Disorder with Intrinsically Disordered Peptide Amphiphiles

Order from disorder with intrinsically disordered peptide amphiphiles

Order from Disorder with Intrinsically Disordered Peptide Amphiphiles

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