Intrinsic exercise capacity is related to differential monoaminergic activity in the rat forebrain

Rabelo, Patrícia; Almeida, Tatiana Fernandes Araújo; Guimarães, Juliana Bohnen; Barcellos, Luiz Alexandre Medrado de; Cordeiro, Letícia Maria de Souza; Moraes, Michele Macedo; Coimbra, Cândido Celso; Szawka, Raphael E.; Soares, Danusa Dias

doi:10.1016/j.brainresbull.2015.01.006

Cited by 25 publications

(34 citation statements)

References 41 publications

(53 reference statements)

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“…In the second week, all rats were subjected to three incremental speed exercises until they were fatigued, with a 48-h interval between tests for recovery. Based on the maximal exercise time (TE max) obtained in one of the three tests, rats were ranked by using a previously described criterion (36,37) into different categories of intrinsic exercise capacity [low-performance (LP), standard-performance (SP) and high-performance (HP)]. Briefly, to be included in the LP group, rats needed to have a TE max lower than 24.9 min, whereas the rats with a TEmax between 24.9 and 57.1 min were ranked in the SP group.…”

Section: Experimental Designmentioning

confidence: 99%

“…In all familiarization sessions and experimental trials, the treadmill inclination was kept at 5 degrees, and an electrical stimulus was set to 0.28 mA. This light electrical stimulation was applied to encourage animals to run (36,37).…”

Section: Familiarization With Treadmill Runningmentioning

confidence: 99%

“…DOPAC and DA. Concentrations of DOPAC and DA were measured in the POA with high-performance liquid chromatography with electrochemical detection, as previously described in Rabelo et al (36,37).…”

Section: Measured Variablesmentioning

confidence: 99%

“…In the beginning of the experiment, there was no intergroup difference in body weight (LP, 283 Ϯ 4 g; SP, 276 Ϯ 4 g; HP, 280 Ϯ 5 g; P ϭ 0.527). The TE max during the incremental speed exercise was 21 Ϯ 2 min, 39 Ϯ 1 min, and 66 Ϯ 1 min for the LP, SP, and HP rats, respectively [P Ͻ 0.001; data published (36)]. Additionally, the S max attained by the rats was 16 Ϯ 0.7 m/min, 21 Ϯ 0.6 m/min, and 31 Ϯ 0.7 m/min for LP, SP, and HP rats, respectively (P Ͻ 0.001).…”

Section: Effect Of Intrinsic Exercise Capacity On Thermoregulation Dumentioning

confidence: 99%

“…Previous experiments have identified that rats exhibit a large intrinsic variation in exercise performance (23)(24)(25) with this heterogeneity being associated with differences in multiple physiological responses (3,19,21,38). In this context, we have previously identified that rats with different intrinsic capacities to exercise show altered monoaminergic responses in the caudateputamen (CPu), also known as the dorsal striatum (36,37). Such neurotransmitter changes might influence mechanical efficiency (ME), metabolic heat production, and the increase in core temperature during exercise (1).…”

Section: Introductionmentioning

confidence: 99%

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Rats with higher intrinsic exercise capacities exhibit greater preoptic dopamine levels and greater mechanical and thermoregulatory efficiencies while running

Rabelo

Cordeiro

Aquino

et al. 2019

Journal of Applied Physiology

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The present study investigated whether intrinsic exercise capacity affects the changes in thermoregulation, metabolism and central dopamine (DA) induced by treadmill running. Male Wistar rats were subjected to three incremental exercises and ranked as low-performance (LP), standard-performance (SP) and high-performance (HP) rats. In the first experiment, abdominal (T) and tail (T) temperatures were registered in these rats during submaximal exercise (SE) at 60% of maximal speed. Immediately after SE, rats were euthanized and concentrations of DA and 3,4-dihydroxyphenylacetic acid (DOPAC) were determined in the preoptic area (POA). In the second experiment, oxygen consumption (VO) was measured and mechanical efficiency (ME) was calculated in these rats during an incremental exercise. HP rats ran for longer periods and fatigued with higher T values, with no difference in T. Nevertheless, thermoregulatory efficiency was higher in HP rats, compared to other groups. DA and DOPAC concentrations in the POA were increased by SE, with higher levels in HP compared to LP and SP rats. VO also differed between groups, with HP rats displaying a lower consumption throughout the incremental exercise but a higher VO at fatigue. ME, in turn, was consistently higher in HP than in LP and SP rats. Thus, our results show that HP rats have greater T values at fatigue, which seem to be related to a higher dopaminergic activity in the POA. Moreover, HP rats exhibited a greater thermoregulatory efficiency during exercise, which that can be attributed to a lower VO, but not to changes in tail heat loss mechanisms.

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Section: Experimental Designmentioning

confidence: 99%

Section: Familiarization With Treadmill Runningmentioning

confidence: 99%

Section: Measured Variablesmentioning

confidence: 99%

Section: Effect Of Intrinsic Exercise Capacity On Thermoregulation Dumentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Rats with higher intrinsic exercise capacities exhibit greater preoptic dopamine levels and greater mechanical and thermoregulatory efficiencies while running

Rabelo

Cordeiro

Aquino

et al. 2019

Journal of Applied Physiology

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Iron deficiency in pregnancy: Influence on sleep, behavior, and molecular markers of adult male offspring

Franco

Mota

Daubian-Nosé

et al. 2021

J of Neuroscience Research

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Iron restriction during pregnancy can lead to iron deficiency and changes in the dopaminergic system in the adulthood of offspring, and restless legs syndrome (RLS) is closely related to these changes. Objectives: Analyze whether iron restriction during pregnancy would cause changes in the behavior, sleep, and dopaminergic system of the male offspring. In addition, we aimed to assess whether exercise would be able to modulate these variables. The pregnant rats (Wistar) were divided into four groups with different concentrations of iron in the diet: standard (St), supplementation (Su), restriction since weaning (R1), and restriction only during pregnancy (R2). After birth, the offspring were assigned to their respective groups according to the dams diet (St, Su, R1, and R2) and distributed into sedentary (SD) and exercised (EX) (for 8 weeks of training), reaching eight groups of offspring (O): OSt SD, OSt EX, OSu SD, OSu EX, OR1 SD, OR1 EX, OR2 SD, and OR2 EX. Sleep, behavior, and analysis of key genes of dopaminergic system (D2, DAT) were performed after 8 weeks. The results for trained offspring that the mother received supplementation diet were the most expressive, with increased freezing and the OR1 SD group showed an increase in DAT protein content. These changes may have been due to the association between the dams diet during pregnancy and the practice of exercise by the offspring. The different concentrations of iron during pregnancy caused changes in the offspring, however, they were not associated with fetal programming in the context of RLS.

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Dopaminergic Modulation of Forced Running Performance in Adolescent Rats: Role of Striatal D1 and Extra-striatal D2 Dopamine Receptors

et al. 2021

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Improving exercise capacity during adolescence impacts positively on cognitive and motor functions. However, the neural mechanisms contributing to enhance physical performance during this sensitive period remain poorly understood. Such knowledge could help to optimize exercise programs and promote a healthy physical and cognitive development in youth athletes. The central dopamine system is of great interest because of its role in regulating motor behavior through the activation of D1 and D2 receptors. Thus, the aim of the present study is to determine whether D1 or D2 receptor signaling contributes to modulate the exercise capacity during adolescence and if this modulation takes place through the striatum. To test this, we used a rodent model of forced running wheel that we implemented recently to assess the exercise capacity. Briefly, rats were exposed to an 8-day period of habituation in the running wheel before assessing their locomotor performance in response to an incremental exercise test, in which the speed was gradually increased until exhaustion. We found that systemic administration of D1-like (SCH23390) and/or D2-like (raclopride) receptor antagonists prior to the incremental test reduced the duration of forced running in a dose-dependent manner. Similarly, locomotor activity in the open field was decreased by the dopamine antagonists. Interestingly, this was not the case following intrastriatal infusion of an effective dose of SCH23390, which decreased motor performance during the incremental test without disrupting the behavioral response in the open field. Surprisingly, intrastriatal delivery of raclopride failed to impact the duration of forced running. Altogether, these results indicate that the level of locomotor response to incremental loads of forced running in adolescent rats is dopamine dependent and mechanistically linked to the activation of striatal D1 and extra-striatal D2 receptors.

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Intrinsic exercise capacity is related to differential monoaminergic activity in the rat forebrain

Cited by 25 publications

References 41 publications

Rats with higher intrinsic exercise capacities exhibit greater preoptic dopamine levels and greater mechanical and thermoregulatory efficiencies while running

Rats with higher intrinsic exercise capacities exhibit greater preoptic dopamine levels and greater mechanical and thermoregulatory efficiencies while running

Iron deficiency in pregnancy: Influence on sleep, behavior, and molecular markers of adult male offspring

Dopaminergic Modulation of Forced Running Performance in Adolescent Rats: Role of Striatal D1 and Extra-striatal D2 Dopamine Receptors

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