Intrinsic control of muscle attachment sites matching

Carayon, Alexandre; Bataillé, Laetitia; Lebreton, Gaëlle; Dubois, L.; Pelletier, Aurore; Carrier, Yannick; Wystrach, Antoine; Víncent, Alaín; Frendo, Jean-Louis

doi:10.7554/elife.57547

Cited by 9 publications

(9 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The posterior lobes are known to express the gene collier/knot, which encodes the Drosophila orthologue of mammalian early B-cell factor (EBF) [22,[37][38][39], essential for B-cell lymphopoiesis in mice [40,41]. To visualize Col expression, we first employed two of the available Gal4: pCol85-Gal4 [7] and Knot 13A11 -Gal4 (GMR13A11/ Kn 13A11 -Gal4: [42,43]) and co-stained them with Col Antibody [44] at 24, 48, 72, 96 AEH and in pre-pupa. Our expression analysis reveals that Kn 13A11 -Gal4 domain co-localizes with high Col expression throughout development (S2A-S2I 00 Fig) . Similar co-localization can be observed with pCol85-Gal4 post 48hrs AEH (S2B-S2J 00 Fig) . It is important to note that the posterior lobes express the Hox gene Ubx [22].…”

Section: Ubx and Collier Expression Changes Dynamically In The Posterior Lobes During Developmentmentioning

confidence: 99%

Ubx-Collier signaling cascade maintains blood progenitors in the posterior lobes of the Drosophila larval lymph gland

et al. 2021

View full text Add to dashboard Cite

Drosophila larval hematopoiesis occurs in a specialized multi-lobed organ called the lymph gland. Extensive characterization of the organ has provided mechanistic insights into events related to developmental hematopoiesis. Spanning from the thoracic to the abdominal segment of the larvae, this organ comprises a pair of primary, secondary, and tertiary lobes. Much of our understanding arises from the studies on the primary lobe, while the secondary and tertiary lobes have remained mostly unexplored. Previous studies have inferred that these lobes are composed of progenitors that differentiate during pupation; however, the mechanistic basis of this extended progenitor state remains unclear. This study shows that posterior lobe progenitors are maintained by a local signaling center defined by Ubx and Collier in the tertiary lobe. This Ubx zone in the tertiary lobe shares several markers with the niche of the primary lobe. Ubx domain regulates the homeostasis of the posterior lobe progenitors in normal development and an immune-challenged scenario. Our study establishes the lymph gland as a model to tease out how the progenitors interface with the dual niches within an organ during development and disorders.

show abstract

Section: Ubx and Collier Expression Changes Dynamically In The Posterior Lobes During Developmentmentioning

confidence: 99%

Ubx-Collier signaling cascade maintains blood progenitors in the posterior lobes of the Drosophila larval lymph gland

et al. 2021

View full text Add to dashboard Cite

show abstract

“…[ 24 ] The FIM setup and the associated tracking software FIMTrack [ 25 ] have been used in a variety of studies which generally track locomotion over few minutes only. [ 26–36 ] Here we present an easy and simple adjustment to our initial FIM setup which allows observation of larvae for hours and for example provides the means to conduct food choice experiments or the analysis of rare locomotor phenotypes.…”

Section: Introductionmentioning

confidence: 99%

Long‐Term Observation of Locomotion of Drosophila Larvae Facilitates Feasibility of Food‐Choice Assays

Bittern¹,

Praetz²,

Baldenius³

et al. 2021

Advanced Biology

View full text Add to dashboard Cite

pin is attached to a torque meter which is placed in the center of a cylindrical panorama. [7] In this flight simulator, the fly experiences a stationary "flight" while the outside world can be adjusted according to the movements recorded by the torque meter. Importantly, this technique can be combined with a cranial window to allow optogenetic stimulation or recording of neuronal activity using genetically encoded sensors.However, the technical challenges of the above methodology prohibit analysis of many animals at the same time. Therefore, slower moving animals such as Drosophila larvae are a well-suited alternative to study locomotion. Mated flies deposit fertilized eggs on the substrate and first instar larvae hatch. They feed on the surface and after one day the second instar is able to start digging into the substrate. To ensure constant air supply, larvae do not dig deeper than one body length and only cooperative behavior in larger groups of larvae allows to reach deeper substrate levels without losing the contiguous air contact at the breathing posterior spiracles. [8][9][10] Thus, larval locomotion can be largely considered to occur in two dimensions.There are numerous published assays for examining larval locomotion which all face the problem of tracking a semi-translucent body on a light-reflective substrate. One approach to circumvent these problems is to feed larvae colored dyes. Alternatively, sophisticated illumination protocols can be employed to increase contrast between larvae and substrate. Several computer-based tracking programs have been developed that can be used to extract larval locomotion from movies taken using CCD or even smartphone cameras. [11][12][13][14][15][16][17][18] To obtain higher spatial and temporal resolution multispectral, high-speed, volumetric swept confocally aligned planar excitation (SCAPE) microscopy has been developed that is capable of analyzing neuronal dynamics during larval locomotion. [19,20] While the different imaging and analysis programs provide a large range of locomotor features and thus allow dissection of complex behavioral phenotypic traits, they lack the ability to track larvae in a larger habitat for a longer time to for example study social interactions or the analysis of rare phenotypes. Long term analysis of locomotor activity is possible but requires keeping the animals in very small containments which restricts possibilities to address the individual behavior. Animals kept in isolation in small and closed containments can be visualized throughout all three larval stages. [21] The correspondingly developed PEDtracker [21] is able to determine the molting time points, but is not able Animal behavior is reflected by locomotor patterns. To decipher the underlying neural circuitry locomotion has to be monitored over often longer time periods. Here a simple adaptation is described to constrain movement of third instar Drosophila larvae to a defined area and use Frustrated total internal reflection based imaging method (FIM) imaging to monitor larva...

show abstract

“…We then asked whether Odd + Col + embryonic pericardial cells contribute to posterior lobe formation. To identify the progeny of these Odd + Col + embryonic pericardial cells, we performed lineage tracing experiments using the odd-Gal4 and the GMR13B08 col-Gal4 drivers, the latter reproducing Col expression in embryonic pericardial cells ( Carayon et al, 2020 ) ( Figure 1I ). Lineage tracing with Odd and Col drivers indicates that nEGFP is expressed in posterior lobe cells, as well as in all larval pericardial cells ( Figures 1H,H’,J,J’ ).…”

Section: Resultsmentioning

confidence: 99%

Identification of Bipotential Blood Cell/Nephrocyte Progenitors in Drosophila: Another Route for Generating Blood Progenitors

Morin-Poulard

Destalminil-Letourneau

Bataillé

et al. 2022

Front. Cell Dev. Biol.

Self Cite

View full text Add to dashboard Cite

The Drosophila lymph gland is the larval hematopoietic organ and is aligned along the anterior part of the cardiovascular system, composed of cardiac cells, that form the cardiac tube and its associated pericardial cells or nephrocytes. By the end of embryogenesis the lymph gland is composed of a single pair of lobes. Two additional pairs of posterior lobes develop during larval development to contribute to the mature lymph gland. In this study we describe the ontogeny of lymph gland posterior lobes during larval development and identify the genetic basis of the process. By lineage tracing we show here that each posterior lobe originates from three embryonic pericardial cells, thus establishing a bivalent blood cell/nephrocyte potential for a subset of embryonic pericardial cells. The posterior lobes of L3 larvae posterior lobes are composed of heterogeneous blood progenitors and their diversity is progressively built during larval development. We further establish that in larvae, homeotic genes and the transcription factor Klf15 regulate the choice between blood cell and nephrocyte fates. Our data underline the sequential production of blood cell progenitors during larval development.

show abstract

Intrinsic control of muscle attachment sites matching

Cited by 9 publications

References 68 publications

Ubx-Collier signaling cascade maintains blood progenitors in the posterior lobes of the Drosophila larval lymph gland

Ubx-Collier signaling cascade maintains blood progenitors in the posterior lobes of the Drosophila larval lymph gland

Long‐Term Observation of Locomotion of Drosophila Larvae Facilitates Feasibility of Food‐Choice Assays

Identification of Bipotential Blood Cell/Nephrocyte Progenitors in Drosophila: Another Route for Generating Blood Progenitors

Contact Info

Product

Resources

About