Intrinsic and extrinsic regulation of the differentiation of skin, corneal and esophageal epithelial cells

Doran, Thomas I.; Vidrich, Alda; Sun, Tung‐Tien

doi:10.1016/0092-8674(80)90150-6

Cited by 272 publications

(119 citation statements)

References 28 publications

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“…Such epithelia differ biochemically in gel electrophoretic pattern of keratin polypetides (Moll et al, 1982;Wu et al, 1982) and histologically (depending upon the species) in expression of a superficial layer of enucleated squames (S. corneum). According to transplantation experiments, this incomplete convergence of differentiated properties evidently reflects intrinsic differences within the epithelial cells rather than specific mesenchymal instruction (Doran et al, 1980), although modulating influences of factors such as vitamin A are demonstrable (Fuchs and Green, 1981). The physiological and molecular bases for differential regulation of properties shared by cells of this type remain to be characterized further.…”

mentioning

confidence: 99%

Rat esophageal and epidermal keratinocytes: Intrinsic differences in culture and derivation of continuous lines

Heimann¹,

Rice²

1983

Journal Cellular Physiology

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Serially cultivated with 3T3 feeder layer support as colonies of stratified squamous epithelium, rat epidermal and esophageal epithelial cells were readily distinguishable by three critera. First, the epidermal colonies, exhibiting extensive piling up of squarnes in the centers, were more stratified than esophageal colonies. Second, in sparse culture 70 to 90% of the esophageal cells but as few as 1 to 5% of the epidermal cells were competent in cross‐linked envelope formation upon treatment with the ionophore X537A. After reaching confluence, up to 90% of the cells of both types formed envelopes upon ionophore treatment. Third, epidermal cells in suspension culture reached maximal levels of spontaneously cross‐linked envelopes in 1 day or less, while esophageal cells required about 4 days in suspension to reach maximal levels. A reproducible finding with both cell types was that initial colony‐forming efficiencies of less than 1% increased to about 40% upon serial passage with consequent derivation of continuous lines. Sparse cultures of esophageal cells with high colony‐forming ability retained a high degree of envelope competence (70 to 90%), indicating these two properties are not mutually exclusive. The derived lines exhibited reduced dependence upon feeder layer support at clonal density, but in suspension culture the cells did not grow and lost colony‐forming ability with a half‐time of several hours. We conclude that cells from these keratinized rat epithelia exhibit intrinsic differences in culture and become continuous lines expressing characteristic regulation of envelope competence and loss of germinative capability in suspension.

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confidence: 99%

Rat esophageal and epidermal keratinocytes: Intrinsic differences in culture and derivation of continuous lines

Heimann¹,

Rice²

1983

Journal Cellular Physiology

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“…the tumour expressed a 'hyperproliferative' keratin pattern, as has been found in encapsulated by a basen specimens examined (da Discussion The SV40 transformed line described in th progression to a malign, unique, we believe, but previously described S (Steinberg & Defendi, al., 1982;Banks-Schlege Hronis et al, 1984;Bei Rhim, 1986; Edelman e human SCCs (Weiss et al, 1984). On re-establishing the tumour cells in vitro, the keratin pattern reverted to one similar to that of cultured SV6-1 Bam/HFK cells, once again emphasising how keratin expression is modulated by the cell's external environment Fuchs & Green, 1981;Doran et al, 1980). Although some differentiation was evident in the tumour, demonstrated by the production of keratin 'pearls' (Figure 3b), no 67 Kd keratin (characteristic of fully keratinized epidermis ) was detected.…”

Section: Keratinsmentioning

confidence: 99%

Malignant progression of an SV40-transformed human epidermal keratinocyte cell line

Gallimore²

1987

Br J Cancer

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Summary Human foetal keratinocytes were transfected with a recombinant plasmid (pSV6-1) which contained an origin defective SV40 genome. The resulting transformed cell line had many properties in common with previously described SV40-transformed keratinocytes, including expression of simple epithelialtype keratins. It was non-tumourigenic in nude mice at early passages, forming small benign cysts, however, after approximately 46 in vitro passages, these transformed keratinocytes formed invasive squamous cell carcinomas in athymic nude mice. Several in vitro changes were associated with this acquisition of tumourigenicity (a) an alteration in cellular morphology, (b) development of a cytogenetically marked clone and (c) loss of cell surface fibronectin. The loss of fibronectin was also observed in vivo; cysts formed by SV6-1 Bam/HFK produced human fibronectin whereas tumours did not, although both tumours and cysts were laminin-and keratin-positive. These results indicate that the spontaneous development of secondary events in immortalised human cells may lead to the acquisition of a malignant phenotype.

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“…CK20 was expressed in the heterotransplanted tumours produced by the CK20-expressing cell lines. It has been reported that patterns of CK expression in cultured cells are not decisively correlated with the CK present in the corresponding tissue Green, 1978, 1981;Sun and Green, 1978;Doran et al, 1980;Wu and Rheinwald, 1981). The fact that CK20 was newly expressed in ovarian carcinoma cells other than mucinous tumours is of clinical importance when we consider the origin of CK20-positive cells.…”

Section: Discussionmentioning

confidence: 94%

“…Secondly, under certain conditions external environmental factors may play an important role in modulating epithelial differentiation. Cultured rabbit skin, corneal and oesophageal epithelial cells have been shown to express more keratin proteins than were originally present in intact tissues or observed in cysts formed by cultured cells in athymic mice (Doran et al, 1980). Thirdly, a new subclone arises from the descendants of the original transformed cell and, with progressive growth in vitro, the cell population becomes enriched with those variants that produce CK20 (Tannock, 1987).…”

Section: Discussionmentioning

confidence: 99%

Complexity of expression of the intermediate filaments of six new human ovarian carcinoma cell lines: new expression of cytokeratin 20

Yanagibashi

Gorai²,

Nakazawa³

et al. 1997

Br J Cancer

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OVSAHO expressed CKs 6, 7, 8,14,18,19 and 15 and/or 16. OVMANA expressed CKs 6, 7, 8, 18,19, 20 and 15 and/or 16. OVKATE expressed CKs 6, 7, 8, 13, 17, 18, 19, 20 and 15 and/or 16. The expression of CK7, additional expression of vimentin, and clinical and histopathological findings enabled us to confirm that six cell lines had been established from primary ovarian cancers. Two of the six cell lines were positive for CK20, although CK20 was not expressed in the original tumours. The heterotransplanted tumours produced by CK20-positive cells also expressed CK20. This is the first report of ovarian carcinoma cell lines that express CK20 irrespective of their histological type. CK20 has been found in all colon carcinoma cell lines, but only in the mucinous type of ovarian tumours. These new ovarian carcinoma cell lines will therefore provide a relevant experimental system for elucidating the regulatory control mechanisms of intermediate filament expression.

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Intrinsic and extrinsic regulation of the differentiation of skin, corneal and esophageal epithelial cells

Cited by 272 publications

References 28 publications

Rat esophageal and epidermal keratinocytes: Intrinsic differences in culture and derivation of continuous lines

Rat esophageal and epidermal keratinocytes: Intrinsic differences in culture and derivation of continuous lines

Malignant progression of an SV40-transformed human epidermal keratinocyte cell line

Complexity of expression of the intermediate filaments of six new human ovarian carcinoma cell lines: new expression of cytokeratin 20

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