Intravital Widefield Fluorescence Microscopy of Pulmonary Microcirculation in Experimental Acute Lung Injury Using a Vacuum-Stabilized Imaging System

Hall, Stefan; Faridi, Sufyan; Euodia, Irene; Tanner, Sophie; Chojnacki, Andrew; Patel, Kamala D.; Lehmann, Christian

doi:10.3791/63733

Cited by 4 publications

(2 citation statements)

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“…Similar results were found by Wu et al (2016), who found that LPS administration significantly increased leukocyte rolling flux and leukocyte adhesion in postcapillary venules of the CNS [19]. These results were later confirmed in a second study conducted by the same research group, finding that LPS administration significantly increased leukocyte rolling flux and leukocyte adhesion compared to saline control [27]. Varying timepoints have been used to assess the inflammatory response to LPS administration.…”

Section: Resultssupporting

confidence: 75%

Intravital Microscopy of Lipopolysaccharide-Induced Inflammatory Changes in Different Organ Systems—A Scoping Review

Scott,

Neira Agonh,

White

et al. 2023

IJMS

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Intravital microscopy (IVM) is a powerful imaging tool that captures biological processes in real-time. IVM facilitates the observation of complex cellular interactions in vivo, where ex vivo and in vitro experiments lack the physiological environment. IVM has been used in a multitude of studies under healthy and pathological conditions in different organ systems. IVM has become essential in the characterization of the immune response through visualization of leukocyte–endothelial interactions and subsequent changes within the microcirculation. Lipopolysaccharide (LPS), a common inflammatory trigger, has been used to induce inflammatory changes in various studies utilizing IVM. In this review, we provide an overview of IVM imaging of LPS-induced inflammation in different models, such as the brain, intestines, bladder, and lungs.

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Section: Resultssupporting

confidence: 75%

Intravital Microscopy of Lipopolysaccharide-Induced Inflammatory Changes in Different Organ Systems—A Scoping Review

Scott,

Neira Agonh,

White

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…Leukocyte trafficking to the lungs is a key component of ALI, so we employed a previously described lung IVM protocol [ 44 ] to study leukocyte rolling and adhesion in venules and arterioles. Although two statistically significant differences were identified, a high degree of variability limited interpretation of overall trends in the data.…”

Section: Discussionmentioning

confidence: 99%

Selective CB2 Receptor Agonist, HU-308, Reduces Systemic Inflammation in Endotoxin Model of Pneumonia-Induced Acute Lung Injury

Hall

Faridi

Trivedi

et al. 2022

IJMS

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Acute respiratory distress syndrome (ARDS) and sepsis are risk factors contributing to mortality in patients with pneumonia. In ARDS, also termed acute lung injury (ALI), pulmonary immune responses lead to excessive pro-inflammatory cytokine release and aberrant alveolar neutrophil infiltration. Systemic spread of cytokines is associated with systemic complications including sepsis, multi-organ failure, and death. Thus, dampening pro-inflammatory cytokine release is a viable strategy to improve outcome. Activation of cannabinoid type II receptor (CB2) has been shown to reduce cytokine release in various in vivo and in vitro studies. Herein, we investigated the effect of HU-308, a specific CB2 agonist, on systemic and pulmonary inflammation in a model of pneumonia-induced ALI. C57Bl/6 mice received intranasal endotoxin or saline, followed by intravenous HU-308, dexamethasone, or vehicle. ALI was scored by histology and plasma levels of select inflammatory mediators were assessed by Luminex assay. Intravital microscopy (IVM) was performed to assess leukocyte adhesion and capillary perfusion in intestinal and pulmonary microcirculation. HU-308 and dexamethasone attenuated LPS-induced cytokine release and intestinal microcirculatory impairment. HU-308 modestly reduced ALI score, while dexamethasone abolished it. These results suggest administration of HU-308 can reduce systemic inflammation without suppressing pulmonary immune response in pneumonia-induced ALI and systemic inflammation.

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Cannabidiol Reduces Systemic Immune Activation in Experimental Acute Lung Injury

Hall,

Faridi,

Trivedi

et al. 2023

Cannabis and Cannabinoid Research

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Intravital Widefield Fluorescence Microscopy of Pulmonary Microcirculation in Experimental Acute Lung Injury Using a Vacuum-Stabilized Imaging System

Cited by 4 publications

References 0 publications

Intravital Microscopy of Lipopolysaccharide-Induced Inflammatory Changes in Different Organ Systems—A Scoping Review

Intravital Microscopy of Lipopolysaccharide-Induced Inflammatory Changes in Different Organ Systems—A Scoping Review

Selective CB2 Receptor Agonist, HU-308, Reduces Systemic Inflammation in Endotoxin Model of Pneumonia-Induced Acute Lung Injury

Cannabidiol Reduces Systemic Immune Activation in Experimental Acute Lung Injury

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