Intravital microscopy at the single vessel level brings new insights of vascular modification mechanisms induced by electropermeabilization

Bellard, Elisabeth; Markelc, Boštjan; Pelofy, Sandrine; Guerroué, François Le; Serša, Gregor; Teissié, Justin; Čemažar, Maja; Golzio, Muriel

doi:10.1016/j.jconrel.2012.09.010

Cited by 63 publications

(63 citation statements)

References 44 publications

(57 reference statements)

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“…As a consequence, thin vessels are likely to be disrupted during any tissue electroporation treatment. This fact most likely provokes the observed disruption of microvasculature flow after electroporation treatments [9], [10] which results in ischemia. Increasing the conductivity of tumor blood could facilitate the destruction of its microvasculature applying lower treatment intensity.…”

Section: B Experimental Resultsmentioning

confidence: 99%

Selective Electroporation of Liver Tumor Nodules by Means of Hypersaline Infusion: A Feasibility Study

et al. 2015

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Abstract-Spread tumors in liver are not suitable to be treated with local treatments, such as conventional surgery or radiofrequency ablation, thus entailing a poor prognosis. Electroporation-based therapies imply the delivery of pulsed high electric fields and currently are performed in a local fashion using needle or plate electrodes. Here, however, it is proposed a novel electroporation paradigm in which field delivery is not local. All the tumor nodules will be selectively treated using large plate electrodes at both sides of the liver. By infusing an hypersaline solution of high electrical conductivity through the portal vein, the electrical conductivity of healthy tissues and tumor nodules will be made significantly different so that the electric field will be focused on the undesirable tissues. Numerical simulations were used to evaluate the feasibility of the proposed technique. In addition, an in vivo procedure was carried out to assess whether it is possible and practical to significantly modify the conductivity of the liver tissue by hypersaline infusion. Both the numerical simulations and the in vivo procedure provided encouraging results.

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Section: B Experimental Resultsmentioning

confidence: 99%

Selective Electroporation of Liver Tumor Nodules by Means of Hypersaline Infusion: A Feasibility Study

et al. 2015

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“…The restoration of blood flow occurred in both types of vessels within 10 minutes after application of electric pulses, however, i.e. blood flow in normal vessels was completely restored in 1 hour, while in tumor vessels even after 24 h the restoration was not completed in all vessels [12,13].…”

Section: Vascular Effects Of Electroporationmentioning

confidence: 88%

Tissue Reactions to Electroporation and Electrochemotherapy: Vascular Effects that have Implications in Tumor Treatment

Serša

Čemažar

2016

IFMBE Proceedings

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Abstract-Electrochemotherapy is an ablative technique utilizing electroporation for enhanced drug delivery to cells. The predominant mechanism of action is enhanced tumor cell cytotoxicity; however, the vascular disrupting effect is also present and is of significant importance. It is limited to tumor microvasculature, while electrochemotherapy does not affect bigger and normal blood vessels. This clinical indication of electrochemotherapy application is important in the treatment of bleeding metastases, since other ablative techniques lack those mechanisms of action. This was confirmed by the clinical data, where electrochemotherapy proved effective and safe in the treatment of metastases located in the vicinity of the major hepatic vessels, not amenable to surgery or radiofrequency ablation.

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“…2c,d,3e). Reversible electroporation may cause blood vessel restriction [17][18][19][20] , and this may be the cause for the red blood vessel agglomeration seen for Protocol D.…”

Section: Discussionmentioning

confidence: 99%

Modulating electrolytic tissue ablation with reversible electroporation pulses

Phillips

Raju²,

Rubinsky

et al. 2015

Technology

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Electrolytic ablation is a minimally invasive tissue ablation technique that operates by delivering low magnitude direct current to the target region over long periods of time, generating electrolytic products that destroy cells. Our study seeks to examine the hypothesis that permeabilizing the cell membrane with reversible electroporation will reduce the electrolytic dose required for tissue ablation, by exposing the interior of the cell to the electrolytic products. The hypothesis is examined by evaluating the extent of tissue damage when electrolytic and reversible electroporation sequences are delivered separately and in combination, in vivo, to rat liver tissue. The study shows that combining reversible electroporation with electrolysis produces a substantial increase in the extent of tissue ablation compared to that achieved by electrolysis alone. INNOVATIONTh is study shows that when reversible electroporation is used to modulate electrolytic ablation, the combination of electrolytic products generated at the electrodes and the permeabilization of the cell membrane greatly increases the extent of tissue ablation. Th us, the electrical parameters typical to reversible electroporation protocols can be harnessed to facilitate cell death. Th is suggests a new technology for tissue ablation. Th e advantage of this technology is that it uses much lower electric fi elds than conventional irreversible electroporation and requires a much lower dose of electrolytic products than typically seen with electrolytic ablation.

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Intravital microscopy at the single vessel level brings new insights of vascular modification mechanisms induced by electropermeabilization

Cited by 63 publications

References 44 publications

Selective Electroporation of Liver Tumor Nodules by Means of Hypersaline Infusion: A Feasibility Study

Selective Electroporation of Liver Tumor Nodules by Means of Hypersaline Infusion: A Feasibility Study

Tissue Reactions to Electroporation and Electrochemotherapy: Vascular Effects that have Implications in Tumor Treatment

Modulating electrolytic tissue ablation with reversible electroporation pulses

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