Intravital imaging of mouse embryos

Huang, Qiang; Cohen, Malkiel A.; Alsina, Fernando C.; Devlin, Garth; Garrett, Aliesha; McKey, Jennifer; Havlik, P.; Rakhilin, Nikolai; Wang, Ergang; Xiang, Kun; Mathews, Parker; Wang, Lihua; Bock, Cheryl B.; Ruthig, Victor A.; Wang, Yi; Negrete, Marcos; Wong, Chi W.; Murthy, Preetish Kadur Lakshminarasimha; Zhang, Shupei; Daniel, Andrea R.; Kirsch, David G.; Kang, Yubin; Capel, Blanche; Asokan, Aravind; Silver, Debra L.; Jaenisch, Rudolf; Shen, Xiling

doi:10.1126/science.aba0210

Cited by 79 publications

(62 citation statements)

References 47 publications

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“…We have developed an in vivo lineage tracing strategy based on Cre-loxp system to observe the target cells in living mouse [29]. Similarly, by taking advantage of a transgenic strains with fluorescent reporters and two-photon microscopy, Huang et al recently reported they observed the development of mouse embryos intravitally [30].…”

Section: Discussionmentioning

confidence: 99%

Fine-scale visualizing the hierarchical structure of mouse biliary tree with fluorescence microscopy method

et al. 2020

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The liver is a vital organ and the hepatic lobule serves as the most basic structural and functional unit which is mainly assembled with parenchymal cells including hepatocytes and biliary epithelial cells. The continuous tubular arrangement of biliary cells which constitutes the biliary tracts is critical for liver function, however, the biliary tracts are often disrupted in many liver diseases such as cirrhosis and some congenital disorders. Visualization of the biliary tracts in fine-scale and three-dimension will help to understanding the structure basis of these liver diseases. In the present study, we established several biliary tract injury mouse models by diet feeding, surgery or genetic modification. The cytoplasm and nuclei of the parenchymal cells were marked by active uptake of fluorescent dyes Rhodamine B (red) and Hoechst (blue), respectively. After the removal of liver en bloc, the biliary tracts were retrogradely perfused with green fluorescent dye, fluorescein isothiocyanate (FITC). The liver was then observed under confocal microscopy. The fine-scale and three-dimensional (3D) structure of the whole biliary tree, particularly the network of the end-terminal bile canaliculi and neighboring hepatocytes were clearly visualized. The biliary tracts displayed clear distinct characteristics in normal liver and diseased liver models. Taken together, we have developed a simple and repeatable imaging method to visualize the fine-scale and hierarchical architecture of the biliary tracts spreading in the mouse liver.

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Section: Discussionmentioning

confidence: 99%

Fine-scale visualizing the hierarchical structure of mouse biliary tree with fluorescence microscopy method

et al. 2020

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“…This powerful approach is increasingly leveraged in experimental and pre-clinical studies to reveal novel insights into the dynamic cellular mechanisms underlying disease development and response to therapy (4,5). To facilitate repeated IVM over prolonged time periods in the same living animal, multiple imaging windows have been designed to provide optical access to internal tissues (6), including the brain (7), skin (8,9), lung (10), mammary gland (6,11,12), abdominal organs (13)(14)(15), femur (16) and embryos (17).…”

Section: Introductionmentioning

confidence: 99%

Longitudinal high-resolution imaging through a flexible intravital imaging window

Jacquemin

Benavente-Diaz

Djaber

et al. 2021

Preprint

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Intravital microscopy (IVM) is a powerful technique that enables imaging of internal tissues at (sub)cellular resolutions in living animals. Here, we present a silicone-based imaging window consisting of a fully flexible, suture-less design that is ideally suited for long-term, longitudinal IVM of growing tissues and tumors. Crucially, we show that this window, without any customization, is suitable for numerous anatomical locations in mice using a rapid and standardized implantation procedure. This low-cost device represents a substantial technological and performance advance that facilitates intravital imaging in diverse contexts in higher organisms, opening new avenues for in vivo imaging of soft and fragile tissues. One-sentence summary: This study presents a versatile, fully flexible imaging window that acts as an implantable transparent "second skin" for small laboratory animal in vivo imaging.

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“…Intravital imaging, the imaging of live animals at microscopic resolution, is gaining increasing attention in biomedicine because it can provide multicolour spatiotemporal information at single-cell resolution in a variety of organs in vivo . In addition to the early imaging of lymph nodes [ 4 , 5 ] and recent visualization of mouse embryos [6] , intravital imaging has become a critical tool to reveal the novel mechanism of nanoparticle transport in the tumour microenvironment [7] , [8] , [9] . Here, we review recent progress on the use of intravital imaging in answering fundamental questions about nanoparticle delivery in vivo .…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle delivery in vivo: A fresh look from intravital imaging

2020

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Nanomedicine has proven promising in preclinical studies. However, only few formulations have been successfully translated to clinical use. A thorough understanding of how nanoparticles interact with cells in vivo is essential to accelerate the clinical translation of nanomedicine. Intravital imaging is a crucial tool to reveal the mechanisms of nanoparticle transport in vivo , allowing for the development of new strategies for nanomaterial design. Here, we first review the most recent progress in using intravital imaging to answer fundamental questions about nanoparticle delivery in vivo . We then elaborate on how nanoparticles interact with different cell types and how such interactions determine the fate of nanoparticles in vivo . Lastly, we discuss ways in which the use of intravital imaging can be expanded in the future to facilitate the clinical translation of nanomedicine.

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Intravital imaging of mouse embryos

Cited by 79 publications

References 47 publications

Fine-scale visualizing the hierarchical structure of mouse biliary tree with fluorescence microscopy method

Fine-scale visualizing the hierarchical structure of mouse biliary tree with fluorescence microscopy method

Longitudinal high-resolution imaging through a flexible intravital imaging window

Nanoparticle delivery in vivo: A fresh look from intravital imaging

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