Intravital imaging of intestinal lacteals unveils lipid drainage through contractility

Choe, Kibaek; Jang, Jeon Yeob; Park, Intae; Kim, Yeseul; Ahn, Soyeon; Park, Dae Young; Hong, Young‐Kwon; Alitalo, Kari; Koh, Gou Young; Kim, Pilhan

doi:10.1172/jci76509

Cited by 97 publications

(114 citation statements)

References 46 publications

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“…39 Furthermore, VEGFC could regulate smooth muscle cell contractions in the villus 150 ; such contractions were recently shown to play an important role in lipid absorption. 38 These findings could open possibilities for the development of new drugs to treat dyslipidemia and obesity. An overview of the lymphatic vessels roles in lipid absorption and transport is presented in Figure 4.…”

Section: Obesitymentioning

confidence: 99%

“…38,39 Most nutrients are absorbed by blood vessels, but the passive diffusion of particles of high molecular weight or colloidal nature is limited across BECs. Therefore, the lacteals are essential for the uptake of dietary fats and fat-soluble vitamins.…”

Section: Absorption Of Dietary Lipidsmentioning

confidence: 99%

“…38 Although the mechanisms of lipid processing by the enterocytes have been explored in detail, the mechanism on how these lipid particles are delivered into the lacteals is still unclear. Early transmission electron microscopic studies have revealed that both passive paracellular and active transcellular transport mechanisms could contribute to lymph production in lacteals.…”

Section: Circulation Researchmentioning

confidence: 99%

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Lymphatic System in Cardiovascular Medicine

Aspelund

Robciuc

Karaman³

et al. 2016

Circulation Research

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266

258

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Abstract:The mammalian circulatory system comprises both the cardiovascular system and the lymphatic system. In contrast to the blood vascular circulation, the lymphatic system forms a unidirectional transit pathway from the extracellular space to the venous system. It actively regulates tissue fluid homeostasis, absorption of gastrointestinal lipids, and trafficking of antigen-presenting cells and lymphocytes to lymphoid organs and on to the systemic circulation. The cardinal manifestation of lymphatic malfunction is lymphedema. Recent research has implicated the lymphatic system in the pathogenesis of cardiovascular diseases including obesity and metabolic disease, dyslipidemia, inflammation, atherosclerosis, hypertension, and myocardial infarction. Here, we review the most recent advances in the field of lymphatic vascular biology, with a focus on cardiovascular disease.

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Section: Obesitymentioning

confidence: 99%

Section: Absorption Of Dietary Lipidsmentioning

confidence: 99%

Section: Circulation Researchmentioning

confidence: 99%

See 1 more Smart Citation

Lymphatic System in Cardiovascular Medicine

Aspelund

Robciuc

Karaman³

et al. 2016

Circulation Research

Self Cite

266

258

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show abstract

“…Within the intestinal tract, each intestinal villus contains a lymphatic lacteal that efficiently absorbs lipids that have been packaged as chylomicrons by enterocytes (2)(3)(4). These intestinal lacteals then drain the lipid-rich chyle fluid to submucosal collecting lymphatic vessels for systemic fat absorption.…”

Section: Introductionmentioning

confidence: 99%

Lymphatic deletion of calcitonin receptor–like receptor exacerbates intestinal inflammation

Davis¹,

Kechele²,

Blakeney³

et al. 2017

JCI Insight

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“…For in vivo video-rate cellular-level monitoring of ICG pharmacokinetic dynamics, a custombuilt video-rate laser-scanning confocal microscopy system previously described [37][38][39][40][41][42] was modified for simultaneous imaging of green and near infrared ICG fluorescence imaging as shown in Fig. 1(a).…”

Section: Near-infrared Laser-scanning Confocal Microscopy Systemmentioning

confidence: 99%

In vivo cellular-level real-time pharmacokinetic imaging of free-form and liposomal indocyanine green in liver

Hwang¹,

Yoon²,

Choe³

et al. 2017

Biomed. Opt. Express

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Indocyanine green (ICG) is a near-infrared fluorophore approved for human use which has been widely used for various clinical applications. Despite the well-established clinical usage, our understanding about the microscopic in vivo pharmacokinetics of systemically administered ICG has been relatively limited. In this work, we successfully visualized real-time in vivo pharmacokinetic dynamics of the intravenously injected free-form and liposomal ICG in cellular resolution by utilizing a custom-built video-rate near infrared laser-scanning confocal microscopy system. Initial perfusion and clearance from blood stream, diffusion into perisinusoidal space, and subsequent absorption into hepatocyte were directly visualized in vivo. The quantification analysis utilizing the real-time image sequences revealed distinct dynamic in vivo pharmacokinetic behavior of free-form and liposomal ICG. Usta, "Long-term maintenance of a microfluidic 3D human liver sinusoid," Biotechnol. Bioeng. 113(1), 241-246 (2016). 46. J. C. Ryan, K. W. Dunn, and B. S. Decker, "Effects of chronic kidney disease on liver transport: quantitative intravital microscopy of fluorescein transport in the rat liver," Am.

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Intravital imaging of intestinal lacteals unveils lipid drainage through contractility

Cited by 97 publications

References 46 publications

Lymphatic System in Cardiovascular Medicine

Lymphatic System in Cardiovascular Medicine

Lymphatic deletion of calcitonin receptor–like receptor exacerbates intestinal inflammation

In vivo cellular-level real-time pharmacokinetic imaging of free-form and liposomal indocyanine green in liver

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