Intravital Imaging of Brain Tumors

Pichol-Thievend, Cathy; Julien, Boris; Anezo, Océane; Philip, Beatrice; Seano, Giorgio

doi:10.1007/978-1-0716-0856-2_4

Cited by 2 publications

(2 citation statements)

References 36 publications

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“…It has been used to study the mechanism of glioblastoma cells in the tumor microenvironment, cellular interactions that contribute to biliary membrane integrity, and fast spatiotemporal cellular dynamics within neural networks. Additionally, it can be used to distinguish tumor-adjacent cancer cells tagged with fluorescent enzymes [6][7][8][9] .…”

Section: Introductionmentioning

confidence: 99%

Stabilized two-photon intravital imaging using dexmedetomidine

Kim¹,

Cho²,

Paulson³

et al. 2023

Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI

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Two-photon microscopy provides subcellular-resolution imaging deep into animal tissues, and is frequently used for the study of biological and disease processes. However, its applicability in vivo is complicated by physiological movement. We demonstrate treatment with dexmedetomidine (DEX), an already FDA-approved alpha-2 adrenergic receptor agonist, to reduce tissue oscillation frequency and amplitude in the livers of anesthetized mice. Fluorescence intensity and focal quality fluctuations were found to improve for 30 minutes after administration, indicating that dexmedetomidine may be used to improve imaging quality in two-photon intravital microscopy studies. These results will likely generalize to other imaging modalities, target tissues, and animal models.

show abstract

Section: Introductionmentioning

confidence: 99%

Stabilized two-photon intravital imaging using dexmedetomidine

Kim¹,

Cho²,

Paulson³

et al. 2023

Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI

View full text Add to dashboard Cite

show abstract

“…Two-photon microscopes enable 3D-resolved fluorescence imaging of highly scattering live cells deep inside thick tissues, as well as the long-term imaging of photosensitive biological specimens with reduced phototoxicity (Denk et al, 1990; Zipfel et al, 2003; Noh et al, 2021). Preclinical observations enabled by the two-photon microscope have included the interaction integrity of glioblastoma cells with the tumor microenvironment (Pichol-Thievend et al, 2021), the cellular interactions which contribute to biliary membrane integrity (Vats et al, 2021), and the fast spatiotemporal cellular dynamics within neural networks (Laffray et al, 2011). Two-photon microscopes have been used to discriminate tumor-adjacent cancer cells tagged with fluorescent enzymes (Noh et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Minimizing Motion Artifacts in Intravital Microscopy Using the Sedative Effect of Dexmedetomidine

Kim

Cho

Paulson

et al. 2022

Microscopy and Microanalysis

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Among intravital imaging instruments, the intravital two-photon fluorescence excitation microscope has the advantage of enabling real-time 3D fluorescence imaging deep into cells and tissues, with reduced photobleaching and photodamage compared with conventional intravital confocal microscopes. However, excessive motion of organs due to involuntary movement such as breathing may result in out-of-focus images and severe fluorescence intensity fluctuations, which hinder meaningful imaging and analysis. The clinically approved alpha-2 adrenergic receptor agonist dexmedetomidine was administered to mice during two-photon fluorescence intravital imaging to alleviate this problem. As dexmedetomidine blocks the release of the neurotransmitter norepinephrine, pain is suppressed, blood pressure is reduced, and a sedation effect is observed. By tracking the quality of focus and stability of detected fluorescence in two-photon fluorescence images of fluorescein isothiocyanate-sensitized liver vasculature in vivo, we demonstrated that intravascular dexmedetomidine can reduce fluorescence fluctuations caused by respiration on a timescale of minutes in mice, improving image quality and resolution. The results indicate that short-term dexmedetomidine treatment is suitable for reducing involuntary motion in preclinical intravital imaging studies. This method may be applicable to other animal models.

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Intravital Imaging of Brain Tumors

Cited by 2 publications

References 36 publications

Stabilized two-photon intravital imaging using dexmedetomidine

Stabilized two-photon intravital imaging using dexmedetomidine

Minimizing Motion Artifacts in Intravital Microscopy Using the Sedative Effect of Dexmedetomidine

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