Intravital imaging-based analysis tools for vessel identification and assessment of concurrent dynamic vascular events

Honkura, Naoki; Richards, Mark; Laviña, Bàrbara; Sáinz‐Jaspeado, Miguel; Betsholtz, Christer; Claesson‐Welsh, Lena

doi:10.1038/s41467-018-04929-8

Cited by 57 publications

(76 citation statements)

References 40 publications

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“…Smooth muscle actin (SMA) staining of the EDL muscle identified arteriolar and venular microvessels and a population of small SMA + vessels having a diameter <10 μm, which we defined as SMA + capillaries and may represent neo‐arterioles (Figure A). The number of SMA + capillaries was elevated in the regenerating muscle of both diet groups, but a substantially greater number was detected in HF diet‐fed mice (Figure B).…”

Section: Resultsmentioning

confidence: 99%

High‐fat diet pre‐conditioning improves microvascular remodelling during regeneration of ischaemic mouse skeletal muscle

et al. 2020

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Aim Critical limb ischaemia (CLI) is characterized by inadequate angiogenesis, arteriolar remodelling and chronic myopathy, which are most severe in type 2 diabetic patients. Hypertriglyceridaemia, commonly observed in these patients, compromises macrovascular function. However, the effects of high‐fat diet‐induced increases in circulating lipids on microvascular remodelling are not established. Here, we investigated if high‐fat diet would mimic the detrimental effect of type 2 diabetes on post‐ischaemia vascular remodelling and muscle regeneration, using a mouse model of hindlimb ischaemia. Methods Male C57Bl6/J mice were fed with normal or high‐fat diets for 8 weeks prior to unilateral femoral artery ligation. Laser doppler imaging was used to assess limb perfusion recovery. Vascular recovery, inflammation, myofibre regeneration and fibrosis were assessed at 4 or 14 days post‐ligation by histology and RNA analyses. Capillary‐level haemodynamics were assessed by intravital microscopy of control and regenerating muscles 14 days post‐ligation. Results High‐fat diet increased muscle succinate dehydrogenase activity and capillary‐level oxygen supply. At 4 days post‐ligation, no diet differences were detected in muscle damage, inflammatory infiltration or capillary activation. At 14 days post‐ligation, high fat‐fed mice displayed accelerated limb blood flow recovery, elevated capillary and arteriole densities as well as greater red blood cell supply rates and capillary‐level oxygen supply. Regenerating muscles from high fat‐fed mice displayed lower interstitial fat and collagen deposition. Conclusion The muscle‐level adaptations to high‐fat diet improved multiple aspects of muscle recovery in response to ischaemia and did not recapitulate the worse outcomes seen in diabetic CLI patients.

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Section: Resultsmentioning

confidence: 99%

High‐fat diet pre‐conditioning improves microvascular remodelling during regeneration of ischaemic mouse skeletal muscle

et al. 2020

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“…However, over time, they extravasate from the blood vessels into tissue, and IVM can be used compute corresponding permeability coefficients and effective diffusion constants. [159] Targeted small molecules have been widely developed as probes for imaging cell populations or extracellular matrix in vivo. As one recent example, a silicone-rhodamine labeled small molecule probe of Mer, a receptor tyrosine kinase highly expressed on macrophages, was recently found to accumulate selectively in Mer+ tumor-associated macrophages (TAMs) in a mouse allograft tumor model.…”

Section: Injectable Reporters For Cell Identificationmentioning

confidence: 99%

Understanding the In Vivo Fate of Advanced Materials by Imaging

Garlin

et al. 2020

Adv Funct Materials

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Engineered materials are ubiquitous in biomedical applications ranging from systemic drug delivery systems to orthopedic implants, and their actions unfold across multiple time‐ and length‐scales. The efficacy and safety of biologics, nanomaterials, and macroscopic implants are all dictated by the same general principles of pharmacology as applied to small molecule drugs, comprising how the body affects materials (pharmacokinetics, PK) and conversely how materials affect the body (pharmacodynamics, PD). Imaging technologies play an increasingly insightful role in monitoring both of these processes, often simultaneously: translational macroscopic imaging modalities such as magnetic resonance imaging and positron emission tomography/computed tomography offer whole‐body quantitation of biodistribution and structural or molecular response, while ex vivo approaches and optical imaging via in vivo (intravital) microscopy reveal behaviors at subcellular resolution. In this review, the authors survey developments in imaging the in situ behavior of systemically and locally administered materials, with a particular focus on using microscopy to understand transport, target engagement, and downstream host responses at a single‐cell level. The themes of microenvironmental influence, controlled drug release, on‐target molecular action, and immune response, especially as mediated by macrophages and other myeloid cells, are examined. Finally, the future directions of how new imaging technologies may propel efficient clinical translation of next‐generation therapeutics and medical devices are proposed.

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“…Transdermal imaging requires no preparation other than affixing a coverslip to the tissue (if a coverslip is required by the objective lens) and immobilizing the region to be imaged. Structures such as the different layers of the skin, hair follicles, and blood vessels; individual cells such as platelets and Langerhans cells; and large proteins and molecules such as thrombin, can all be directly visualized over time.…”

Section: Intravital Imaging Visualizes In Vivo Cellular Dynamics and mentioning

confidence: 99%

Intravital Imaging Techniques for Biomedical and Clinical Research

et al. 2019

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Intravital imaging, the direct visualization of cells and tissues within a living animal, is a technique that has been employed for the better part of a century. The advent of confocal and multiphoton microscopy has dramatically improved the power of intravital imaging, making it possible to obtain optical sections of tissues non-destructively. This review discusses the various techniques used for intravital imaging, describes how intravital imaging provides information about cellular and tissue dynamics not possible to be garnered by other techniques, and details several ways in which intravital imaging is making a direct impact on the clinical care of patients.

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Intravital imaging-based analysis tools for vessel identification and assessment of concurrent dynamic vascular events

Cited by 57 publications

References 40 publications

High‐fat diet pre‐conditioning improves microvascular remodelling during regeneration of ischaemic mouse skeletal muscle

High‐fat diet pre‐conditioning improves microvascular remodelling during regeneration of ischaemic mouse skeletal muscle

Understanding the In Vivo Fate of Advanced Materials by Imaging

Intravital Imaging Techniques for Biomedical and Clinical Research

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