Intravenous transfusion of endothelial colony-forming cells attenuates vascular degeneration after cerebral aneurysm induction

Li, Shengjie; Tian, Ye; Huang, Xintao; Zhang, Yongqiang; Wang, Dehui; Wei, Huijie; Dong, Jing‐fei; Jiang, Rongcai; Zhang, Jianning

doi:10.1016/j.brainres.2014.09.077

Cited by 30 publications

(27 citation statements)

References 59 publications

(70 reference statements)

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“…We then assessed the BBB permeability and found albumin attenuated junction protein degradation and BBB breakdown. Mechanically, aberrant gelatinases (MMP-2 and MMP-9) dissociate the basal lamina and astrocytic endfeet and impair synapsis number and synaptic activity (28), thus being a principal perpetrator disrupting neurovascular unit after SAH (29). We herein employed immunoblotting and zymography to assess the expression and activity of gelatinases in the ipsilateral hemisphere to the filament perforation.…”

Section: Discussionmentioning

confidence: 99%

Human Albumin Improves Long-Term Behavioral Sequelae After Subarachnoid Hemorrhage Through Neurovascular Remodeling

Xie

Liu

Zhang

et al. 2015

Critical Care Medicine

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Section: Discussionmentioning

confidence: 99%

Human Albumin Improves Long-Term Behavioral Sequelae After Subarachnoid Hemorrhage Through Neurovascular Remodeling

Xie

Liu

Zhang

et al. 2015

Critical Care Medicine

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“…This anti-inflammatory effect of ECFCs may be associated with inhibition of chemokines (MCP-1 and ICAM-1) [11] and regulation of the immune response [26]. The regulation of ECFCs on inflammation was mainly attributed to the inhibition of NF-kB [27]. Moreover, the capacity of ECFCs to induce antiinflammatory IL-10 also played an important role.…”

Section: Discussionmentioning

confidence: 99%

Endothelial colony-forming cells reduced the lung injury induced by cardiopulmonary bypass in rats

Sun

Zhao

Tai

et al. 2020

Preprint

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Background: Cardiopulmonary bypass (CPB) results in severe lung injury via inflammation and endothelial injury. The aim of this study was to evaluate the effect of endothelial colony-forming cells (ECFCs) on lung injury in rats subjected to CPB. Methods: Thirty-two rats were randomized into the sham, CPB, CPB/ECFC and CPB/ECFC/L-NIO groups. The rats in the sham group received anaesthesia, and the rats in the other groups received CPB. The rats also received PBS, ECFCs and L-NIO-pretreated ECFCs. After 24 hours of CPB, pulmonary capillary permeability, including the PaO 2 /FiO 2 ratio, protein levels in bronchoalveolar lavage fluid (BALF) and lung tissue wet/dry weight, was evaluated. The cell numbers and cytokines in BALF and peripheral blood were tested. Endothelial injury, lung histological injury and apoptosis were assessed. The oxidative stress response and apoptosis-related proteins were analysed. Results: After CPB, all the data deteriorated compared with those obtained in the S group (sham vs CPB vs CPB/ECFC vs CPB/ECFC/L-NIO: histological score: 1.62±0.51 vs 5.37±0.91 vs 3.37±0.89 vs 4.37±0.74; PaO 2 /FiO 2 : 389±12 vs 233±36 vs 338±28 vs 287±30; wet/dry weight: 3.11±0.32 vs 6.71±0.73 vs 4.66±0.55 vs 5.52±0.57; protein levels: 134±22 vs 442±99 vs 225±41 vs 337±53, all P<0.05). Compared to the CPB treatment, ECFCs significantly improved pulmonary capillary permeability and PaO 2 /FiO 2 . Similarly, ECFCs also decreased the inflammatory cell number and pro-inflammatory factors in BALF and peripheral blood, as well as the oxidative stress response in the lung tissue. ECFCs reduced the lung histological injury score and apoptosis and regulated apoptosis-related proteins in the lung tissue. Compared with CPB/ECFC group, all the indicators were partly reversed by the L-NIO. Conclusions: ECFCs significantly reduced lung injury induced by inflammation after CPB.

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“…Positive surface expression of CD105 and CD146 were reported in 35% and 42% of studies, respectively. Only 11 studies (17%) met the complete Medina criteria, 18,19,29,[33][34][35][36][37][38][39][40] with just four studies (6%) 19,29,34,38 reporting complete characterization information within the primary article (Supporting Information 2).…”

Section: Risk Of Biasmentioning

confidence: 99%

“…Some organ injury categories included multiple types of organ dysfunction, while other categories were more homogenous in the induction of organ dysfunction. In particular, hind limb ischemia (n = 23), [16][17][18]21,22,32,33,36,39,[44][45][46][47][48][49][50][51][52][53][54][55][56][57] cerebral ischemia (n = 7), 12,37,[58][59][60][61][62] and acute renal injury (n = 6) 11,23,26,27,30,63 studies represent the three most common models of organ dysfunction (Table 4) Multiple system refers to composite measures such as exercise capacity and changes in weight that cannot be isolated to an individual organ system. b Some studies reported on outcomes related to one or more organ systems and the total number of studies is greater than 66.…”

Section: Organ System Injury Models and Outcome Reportingmentioning

confidence: 99%

Human endothelial colony-forming cells in regenerative therapy: A systematic review of controlled preclinical animal studies

Liao

Zheng

Shorr

et al. 2020

Stem Cells Translational Medicine

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Endothelial colony-forming cells (ECFCs) hold significant promise as candidates for regenerative therapy of vascular injury. Existing studies remain largely preclinical and exhibit marked design heterogeneity. A systematic review of controlled preclinical trials of human ECFCs is needed to guide future study design and to accelerate clinical translation. A systematic search of Medline and EMBASE on 1 April 2019 returned 3131 unique entries of which 66 fulfilled the inclusion criteria. Most studies used ECFCs derived from umbilical cord or adult peripheral blood. Studies used genetically modified immunodeficient mice (n = 52) and/or rats (n = 16). ECFC phenotypes were inconsistently characterized. While >90% of studies used CD31+ and CD45−, CD14 − was demonstrated in 73% of studies, CD146+ in 42%, and CD10+ in 35%. Most disease models invoked ischemia. Peripheral vascular ischemia (n = 29), central nervous system ischemia (n = 14), connective tissue injury (n = 10), and cardiovascular ischemia and reperfusion injury (n = 7) were studied most commonly. Studies showed predominantly positive results; only 13 studies reported ≥1 outcome with null results, three reported only null results, and one reported harm. Quality assessment with SYRCLE revealed potential sources of bias in most studies. Preclinical ECFC studies are associated with benefit across several ischemic conditions in animal models, although combining results is limited by marked heterogeneity in study design. In particular, characterization of ECFCs varied and aspects of reporting introduced risk of bias in most studies. More studies with greater focus on standardized cell characterization and consistency of the disease model are needed.

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Intravenous transfusion of endothelial colony-forming cells attenuates vascular degeneration after cerebral aneurysm induction

Cited by 30 publications

References 59 publications

Human Albumin Improves Long-Term Behavioral Sequelae After Subarachnoid Hemorrhage Through Neurovascular Remodeling

Human Albumin Improves Long-Term Behavioral Sequelae After Subarachnoid Hemorrhage Through Neurovascular Remodeling

Endothelial colony-forming cells reduced the lung injury induced by cardiopulmonary bypass in rats

Human endothelial colony-forming cells in regenerative therapy: A systematic review of controlled preclinical animal studies

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