Intravenous infusion of bone marrow-derived mesenchymal stem cells improves tissue perfusion in a rat hindlimb ischemia model

Abstract

Intravenous infusion of stem cells is a minimally invasive cellular delivery method, though a few have been reported in a critical limb-threatening ischemia (CLTI) animal model or patients. In the present study, we hypothesized that intravenous infusion of bone-marrow derived mesenchymal stem cells (MSCs) improves tissue perfusion in a rat hindlimb ischemia model. Hindlimb ischemia was generated in Sprague–Dawley rats by femoral artery removal, then seven days after ischemic induction intravenous infusion of 1 × 106 MSCs (cell group) or vehicle (control group) was performed. As compared with the control, tissue perfusion was significantly increased in the cell group. Histological findings showed that capillary density was significantly increased in the cell group, with infused green fluorescent protein (GFP)-MSCs distributed in the ischemic limb. Furthermore, gene expression of vascular endothelial growth factor (VEGF) was significantly increased in ischemic hindlimb muscle tissues of rats treated with MSC infusion. In conclusion, intravenous infusion of bone-marrow derived MSCs improved tissue perfusion in ischemic hindlimbs through angiogenesis, suggesting that intravenous infusion of MSCs was a promising cell delivery method for treatment of CLTI.

Mimicking microbone tissue by 3-dimensional printing

“…Polymers are commonly used as synthetic materials for creating scaffolds, with linear aliphatic polyesters such as PGA, PLA, and PLGA being the most widely used [39]. Additionally, biocompatible polymers like polyether ether ketone, PCL, and PLA are utilized in tissue engineering to replace bone tissue due to their biodegradable, non-toxic, non-immunogenic, and non-inflammatory properties [55]. Ledda et al [50] proposed the use of 3D-printed PLA scaffolds with a microstructure inspired by trabecular bone architecture to support the adhesion, growth, and differentiation of osteoblast-like cells and microporous structures.…”

The neuroprotective potential of mesenchymal stem cells from bone marrow and human exfoliated deciduous teeth in a murine model of demyelination

“…MSCs can migrate towards sites of injury [10], where they are able to adapt to the local environment and promote protection and regeneration of myelin and neurons, thus leading to improved functional outcomes in models of CNS diseases. [11][12][13] This effect is likely mediated through different mechanisms, such as the paracrine stimulation of endogenous progenitor cells and stem cells through their secretome [14], mitochondria donation [15], immunomodulation [16] and transdifferentiation in neural and oligodendroglial direction [17].…”