Intravenous administration of multipotent stromal cells prevents the onset of non-alcoholic steatohepatitis in obese mice with metabolic syndrome

Ezquer, Marcelo; Ezquer, Fernando; Ricca, M; Allers, Carolina; Conget, Paulette

doi:10.1016/j.jhep.2011.02.020

Cited by 69 publications

(72 citation statements)

References 51 publications

(56 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The role of MSCs in fi brosis is still a matter of controversy. Some studies have indicated that MSCs have no eff ect, others show an increase and others show a decrease (Carvalho et al, 2008;di Bonzo et al, 2008;Ezquer et al, 2011). These diff erences may be related, at least in part, to the characteristics of target tissues, fi brosis etiology, the stage of disease at the moment of MSC administration and follow up time.…”

Section: Stem Cell-based Strategies To Manage Patients With Diabetic mentioning

confidence: 99%

MSC transplantation: a promising therapeutic strategy to manage the onset and progression of diabetic nephropathy

Ezquer

Arango-Rodríguez

et al. 2012

Biol. Res.

View full text Add to dashboard Cite

Currently, one of the main threats to public health is diabetes mellitus. Its most detrimental complication is diabetic nephropathy (DN), a clinical syndrome associated with kidney damage and an increased risk of cardiovascular disease. Irrespective of the type of diabetes, DN follows a well-known temporal course. The earliest detectable signs are microalbuminuria and histopathological changes including extracellular matrix deposition, glomerular basement membrane thickening, glomerular and mesangial expansion. Later on macroalbuminuria appears, followed by a progressive decline in glomerular fi ltration rate and the loss of glomerular podocytes, tubulointerstitial fi brosis, glomerulosclerosis and arteriolar hyalinosis. Tight glycemic and hypertension controls remain the key factors for preventing or arresting the progression of DN. Nevertheless, despite considerable educational eff ort to control the disease, a signifi cant number of patients not only develop DN, but also progress to chronic kidney disease. Therefore, the availability of a strategy aimed to prevent, delay or revert DN would be highly desirable. In this article, we review the pathophysiological features of DN and the therapeutic mechanisms of multipotent mesenchymal stromal cells, also referred to as mesenchymal stem cells (MSCs). The perfect match between them, together with encouraging pre-clinical data available, allow us to support the notion that MSC transplantation is a promising therapeutic strategy to manage DN onset and progression, not only because of the safety of this procedure, but mainly because of the renoprotective potential of MSCs.

show abstract

Section: Stem Cell-based Strategies To Manage Patients With Diabetic mentioning

confidence: 99%

MSC transplantation: a promising therapeutic strategy to manage the onset and progression of diabetic nephropathy

Ezquer

Arango-Rodríguez

et al. 2012

Biol. Res.

View full text Add to dashboard Cite

show abstract

“…The diet used was elected because it is standardized, commercially available, supplies levels of fat higher than those recommended in a human healthy diet (60 vs. 25% calories from fat, respectively) [14], and proved to induce obesity and non-alcoholic steatohepatitis in C57BL/6 mice. [15], [16]. After 8, 12 and 16 months under this regimen (obese), metabolic, vascular and cardiac features were assessed, and compared with sex- and age-matched mice fed with regular diet (normal).…”

Section: Introductionmentioning

confidence: 99%

Mice Long-Term High-Fat Diet Feeding Recapitulates Human Cardiovascular Alterations: An Animal Model to Study the Early Phases of Diabetic Cardiomyopathy

et al. 2013

Self Cite

View full text Add to dashboard Cite

Background/AimHypercaloric diet ingestion and sedentary lifestyle result in obesity. Metabolic syndrome is a cluster of clinical features secondary to obesity, considered as a pre-diabetic condition and recognized as an independent risk factor for cardiovascular diseases. To better understand the relationship between obesity, metabolic syndrome and cardiovascular disease as well as for the development of novel therapeutic strategies, animal models that reproduce the etiology, course and outcomes of these pathologies are required. The aim of this work was to characterize the long-term effects of high-fat diet-induced obesity on the mice cardiovascular system, in order to make available a new animal model for diabetic cardiomyopathy.Methods/ResultsMale C57BL/6 mice were fed with a standardized high-fat diet (obese) or regular diet (normal) for 16 months. Metabolic syndrome was evaluated testing plasma glucose, triglycerides, cholesterol, insulin, and glucose tolerance. Arterial pressure was measured using a sphygmomanometer (non invasive method) and by hemodynamic parameters (invasive method). Cardiac anatomy was described based on echocardiography and histological studies. Cardiac function was assessed by cardiac catheterization under a stress test. Cardiac remodelling and metabolic biomarkers were assessed by RT-qPCR and immunoblotting. As of month eight, the obese mice were overweight, hyperglycaemic, insulin resistant, hyperinsulinemic and hypercholesterolemic. At month 16, they also presented normal arterial pressure but altered vascular reactivity (vasoconstriction), and cardiac contractility reserve reduction, heart mass increase, cardiomyocyte hypertrophy, cardiac fibrosis, and heart metabolic compensations. By contrast, the normal mice remained healthy throughout the study.ConclusionsMice fed with a high-fat diet for prolonged time recapitulates the etiology, course and outcomes of the early phases of human diabetic cardiomyopathy.

show abstract

“…In animal models, we can also track the cells by using techniques of fluorescent labeling [152][153][154][155] , transduction of MSCs with reporter genes [59] , taking advantage of species or sex mismatch by using specific genes as markers by fluorescent in situ hybridization or real-time PCR [11,24] . In addition, nanoparticles have been used as a noninvasive technique of in vivo cellular tracking in cell therapy.…”

Section: Tracking Migration and Distribution Of Implanted Mscsmentioning

confidence: 99%

Strategies to improve the migration of mesenchymal stromal cells in cell therapy

Chen

et al. 2017

Transl. Neurosci. Clin.

View full text Add to dashboard Cite

KEYWORDSMSCs; migration; cell therapy; engraftment efficiency; neurological disorder Mesenchymal stromal/stem cells (MSCs) are multipotent cells under consideration as a potential new therapy for a variety of inflammatory diseases including certain neurological disorders. It is generally thought that the efficacy of cell therapy in attenuating damage after ischemia, inflammation, or injury depends on the quantity of transplanted cells recruited to the target tissue. However, only a small number of systematically infused MSCs can effectively migrate to target sites, which significantly decreases the efficacy of exogenous cell-based therapy. In this review, we discuss specific factors influencing MSC migration, and summarize current strategies that effectively promote the motility of MSCs. In addition, we describe several protocols to improve the migration of stromal cells into the nervous system and, therefore, enhance the efficiency of engraftment as means of treating neurological disorders.Citation Li G, Hu Y, Chen Y, Tang Z. Strategies to improve the migration of mesenchymal stromal cells in cell therapy. Transl. Neurosci. Clin. 2017, 3(3): 159-175.

show abstract

Intravenous administration of multipotent stromal cells prevents the onset of non-alcoholic steatohepatitis in obese mice with metabolic syndrome

Cited by 69 publications

References 51 publications

MSC transplantation: a promising therapeutic strategy to manage the onset and progression of diabetic nephropathy

MSC transplantation: a promising therapeutic strategy to manage the onset and progression of diabetic nephropathy

Mice Long-Term High-Fat Diet Feeding Recapitulates Human Cardiovascular Alterations: An Animal Model to Study the Early Phases of Diabetic Cardiomyopathy

Strategies to improve the migration of mesenchymal stromal cells in cell therapy

Contact Info

Product

Resources

About