Intravenous administration of human amniotic mesenchymal stem cells improves outcomes in rats with acute traumatic spinal cord injury.

Abstract

We previously reported that intraspinal transplantation of human amniotic mesenchymal stem cells (hAMSCs) promotes functional recovery in a rat model of acute traumatic spinal cord injury (SCI). However, whether intravenous transplantation of hAMSCs also has therapeutic benefit remains uncertain. In this study, we assessed whether intravenous transplantation of hAMSCs improves outcomes in rats with acute traumatic SCI. In addition, the potential mechanisms underlying the possible benefits of this therapy were investigated. Adult female Sprague–Dawley rats were subjected to SCI using a weight drop device, and then hAMSCs or PBS were administered after 2 h via the tail vein. Our results indicated that transplanted hAMSCs could migrate to injured spinal cord lesion. Compared with the control group, hAMSCs transplantation significantly decreased the numbers of ED1+ macrophages/microglia and caspase-3+ cells, and reduced levels of inflammatory cytokines, such as tumor necrosis factor alpha, interleukin-6 and IL-1β. In addition, hAMSCs transplantation significantly attenuated Evans blue extravasation, promoted angiogenesis and axonal regeneration. hAMSCs transplantation also significantly improved functional recovery. These results suggest that intravenous administration of hAMSCs provides neuroprotective effects in rats after acute SCI, and could be an alternative therapeutic approach for the treatment of acute SCI.

Characteristics and Therapeutic Potential of Human Amnion-Derived Stem Cells

“…To date, no effective treatment is satisfactory for the recovery of this type of neurological disease. Zhou et al observed that there was a neuroprotection in SCI rats by injections of hAMSCs nearby the injury site and intravenous transplantation and the possible mechanism might be related with promoting the regeneration of axons and angiogenesis [91]. Sankar et al reported that hAESCs graft prevented the formation of glial scars in the transection lesion site and inhibited the apoptosis of axotomized neurons [92].…”

Mesenchymal stem cell‐derived exosomes improve neurogenesis and cognitive function of mice with methamphetamine addiction: A novel treatment approach

“…15 MSCs substantially decrease the production of pro-inflammatory cytokines, such as TNFα, IL-6, and IL-1β, while boosting the levels of M2 macrophages and anti-inflammatory cytokines, such as IL-10, in the injured brain tissue. 16 These cells also activate the BDNF/TrkB/ CREB signaling pathway, leading to elevated levels of DCX, PSD-95, and synaptophysin, thereby proving the occurrence of neurogenesis and synaptogenesis. 17 MSC-derived exosomes are small lipid vesicles (30 to 150 nm in diameter) that are loaded with numerous biological components, including mRNA, miRNA, proteins, cytokines, lipids, and other molecules.…”

Paclitaxel-incorporated nanoparticles improve functional recovery after spinal cord injury

“…Spinal cord injury, one of the central nervous system injuries, usually leads to substantial neurological dysfunction and permanent sensory-motor disabilities ( French et al, 2007 ; Jiang et al, 2020 ; Zhou et al, 2020 ). Injury to the spinal cord affects approximately 180,000 patients worldwide every year, which has a negative impact on the living quality of patients and increases the financial burden ( Fitzhar ris et al, 2014 ).…”