Intravascular hemolysis induced by Lonomia obliqua caterpillar bristle extract: an experimental model of envenomation in rats

The Study to Reduce the Hemolysis Side Effect of Puerarin by a Submicron Emulsion Delivery System

“…8,9) It is well known that the number of erythrocytes must decrease significantly in vivo as soon as to occur the intravenous hemolysis, meanwhile hemoglobin levels and plasma haptoglobin levels decreased significantly, and plasma hemoglobin will increase abnormally. 23) Our results showed that the intravascular hemolysis occurred at 42 d, 43 d in the rabbits injected i.d. 25 mg puerarin of puerarin i.v.…”

Acute Kidney Injury Induced by Snake and Arthropod Venoms

“…Clinical symptoms of Lonomia envenomation include local pain (burning sensation) and inflammatory reaction, which starts immediately after contact; systemic reactions such as headache, fever, vomiting and asthenia, which appear a few hours after exposure; and bleeding diathesis characterized by hematomas and ecchymosis, gross hematuria, hematemesis, melena, pulmonary and intracerebral hemorrhage and AKI (Pinto et al, 2010). Intravascular hemolysis has also been described in human envenomation and experimental studies (Malaque et al, 2006;Seibert et al, 2004). The activation of blood coagulation, fibrinolysis and the systemic inhibition of platelet function are mechanisms that seem to contribute to the hemorrhagic syndrome commonly observed in Lonomia envenomation (Berger et al, 2010a).…”

“…The phospholipase A2 enzyme also has a function in envenomation. This enzyme was isolated and characterized as the major component responsible to the in vitro and in vivo hemolytic activity of L. obliqua venom (Seibert et al, 2004;Seibert et al, 2006). Additionally, the phospholipase A2 seems to be involved in platelet aggregation inducing activity present in the venom (Berger et al, 2010b).…”

A catalog for the transcripts from the venomous structures of the caterpillar Lonomia obliqua: Identification of the proteins potentially involved in the coagulation disorder and hemorrhagic syndrome

“…Of note, L. obliqua bristle extract induces in vitro hemolysis on rat and human erythrocytes as well as intravascular hemolysis in rats, probably due to a PLA2 (Seibert et al, 2004). Both cDNA libraries contain sequences homologous to PLA2 and other proteins that may have hemolytic activity.…”

“…Furthermore, LOqua-PLA1 also displays homology to the carboxy domain of human Group III PLA2 (gi| 7657126) whose function is related to arachidonate release from cells (Murakami et al, 2003). At present, the substrate specificity and function for this putative secreted PLA2 is a matter of speculation, but it may be somehow involved in the hemolysis observed after envenomation (Seibert et al, 2004).…”