Intrauterine inflammation exacerbates maladaptive remodeling of the immature myocardium after preterm birth in lambs

Abstract

Background Antenatal conditions that are linked with preterm birth, such as intrauterine inflammation, can influence fetal cardiac development thereby rendering the heart more vulnerable to the effects of prematurity. We aimed to investigate the effect of intrauterine inflammation, consequent to lipopolysaccharide exposure, on postnatal cardiac growth and maturation in preterm lambs. Methods Preterm lambs (~129 days gestational age) exposed antenatally to lipopolysaccharide or saline were managed according to contemporary neonatal care and studied at postnatal day 7. Age-matched fetal controls were studied at ~136 days gestational age. Cardiac tissue was sampled for molecular analyses and assessment of cardiac structure and cardiomyocyte maturation. Results Lambs delivered preterm showed distinct ventricular differences in cardiomyocyte growth and maturation trajectories as well as remodeling of the left ventricular myocardium compared to fetal controls. Antenatal exposure to lipopolysaccharide resulted in further collagen deposition in the left ventricle and a greater presence of immune cells in the preterm heart. Conclusions Adverse impacts of preterm birth on cardiac structure and cardiomyocyte growth kinetics within the first week of postnatal life are exacerbated by intrauterine inflammation. The maladaptive remodeling of the cardiac structure and perturbed cardiomyocyte growth likely contribute to the increased vulnerability to cardiac dysfunction following preterm birth. Impact Preterm birth induces maladaptive cardiac remodeling and adversely impacts cardiomyocyte growth kinetics within the first week of life in sheep. These effects of prematurity on the heart are exacerbated when preterm birth is preceded by exposure to intrauterine inflammation, a common antecedent of preterm birth. Inflammatory injury to the fetal heart coupled with preterm birth consequently alters neonatal cardiac growth and maturation and thus, may potentially influence long-term cardiac function and health.

In Utero Exposure to Maternal SARS-CoV-2 Infection Is Associated With Higher Left Ventricular Mass in Toddlers

“…Maternal inflammation has been posited as a central mechanism in the fetal programming of cardiometabolic disease [ 2 , 32 ]. In this regard, sheep exposed to maternal inflammation in late gestation exhibited hearts with impaired structure and function and higher collagen deposition [ 33 , 34 ]. In the current study, we analyzed relationships of LVMI with maternal immunologic indices derived from a complete blood count.…”

The role of interleukin-1 in perinatal inflammation and its impact on transitional circulation

“…injection of LPS 48 h before delivery exhibited impaired cardiomyocyte growth, increased collagen deposition and remodeling of the left ventricular myocardium, when compared to saline-treated controls. Affected lambs also demonstrated increased expression of genes related to cardiac metabolism and calcium handling, however expression of IL1B was not significantly increased ( 107 ). In non-human primates, cardiac tissue exposed to intrauterine infection identified reduced gene expression of pathways involved in cardiac morphogenesis and vasculogenesis ( 108 ).…”

Breastfeeding and Rotational Mechanics of the Heart during Postnatal Ontogenesis in Children (A Literature Review)

“…Среди важнейших факторов в условиях преждевременных родов в анамнезе, определяющих генез контрактильно-ротационных процессов, включая возникновение «реверсивного» варианта движения в систолу апикальных отделов, выделяются не только фиброз и гипертрофия ЛЖ [64,75], но и весьма вероятен вклад в механику ЛЖ хаотичного расположения мышечных волокон [64], уменьшенного количества [75] и незавершенной дифференцировки кардиомиоцитов [64].…”