Intrauterine infection with Ureaplasma urealyticum as a cause of fatal neonatal pneumonia

Quinn, Patrick G.; Gillan, John; Markestad, Trond; John, Martha; Daneman, Alan; Lie, K.I.; Li, H C; Czegledy-Nagy, E; Klein, Anke

doi:10.1097/00006454-198509000-00020

Cited by 77 publications

(36 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Increases in BAL and interstitial neutrophils and macrophages were again evident during the chronic phase (days 14 to 28). This is similar to histologic findings in experimental animal models of U. urealyticum infection (8,39,46) and in human Ureaplasma-infected preterm (43) and full-term (6,36,45) infants dying with acute pneumonia. Murine models of Mycoplasma pneumoniae demonstrated a different pattern of inflammation.…”

Section: Discussionsupporting

confidence: 85%

“…The histopathologic findings of our juvenile-mouse model are similar to those observed in stillborn (13) and full-term human infants (6,36,45) and in a newborn primate model of Ureaplasma pneumonia (46). A hallmark feature in these studies is the focal loss of ciliated respiratory epithelium.…”

Section: Discussionsupporting

confidence: 77%

“…A hallmark feature in these studies is the focal loss of ciliated respiratory epithelium. Immunofluorescence of term infant lung infected with Ureaplasma serotype 8 (biovar 2) revealed adherence of organisms in fine clumps and strands along alveoli and terminal bronchioles (36). In a second case report of a Ureaplasma-infected stillborn fetus, scanning electron microscopy of the trachea showed focal areas of decreased ciliated epithelial cells, with residual cilia being clumped or flattened (13).…”

Section: Discussionmentioning

confidence: 95%

See 2 more Smart Citations

Characterization of a Murine Model ofUreaplasma urealyticumPneumonia

Viscardi¹,

Kaplan²,

et al. 2002

Infect Immun

View full text Add to dashboard Cite

Ureaplasma urealyticum respiratory tract colonization in preterm infants has been associated with a high incidence of pneumonia and the development of bronchopulmonary dysplasia. However, study of this human pathogen has been hampered by the absence of animal models. We have developed the first juvenile mouse model of Ureaplasma pneumonia and characterized the histopathology during the month following inoculation. C3H/ HeN mice were inoculated intratracheally with a mouse-adapted clinical Ureaplasma isolate (biovar 2) or sham inoculated with 10B broth. Culture of lung homogenates and PCR of DNA from bronchoalveolar lavage fluid (BAL) confirmed the presence of Ureaplasma in 100% of inoculated animals at 1 day, 60% at 2 days, 50% at 3 days, and 25% at 7 and 14 days. Ureaplasma was undetectable 28 days postinoculation. There were marked changes in BAL and interstitial-cell composition with increased number of polymorphonuclear leukocytes 1 to 2 days and 14 days postinoculation and macrophages at 2 and 14 days postinoculation. The Ureaplasma infection caused a persistent focal loss of airway ciliated epithelium and a mild increase in interstitial cellularity. There were no differences in BAL protein concentration during the first 28 days, suggesting that pulmonary vascular endothelial barrier integrity remained intact. Comparison of BAL cytokine and chemokine concentrations revealed low levels of tumor necrosis factor alpha (TNF-␣) at 3 days and monocyte chemoattractant protein 1 at 7 days in Ureaplasma-infected mice but a trend toward increased TNF-␣ at 14 days and increased granulocyte-macrophage colony-stimulating factor and interleukin-10 at 28 days. These data suggest that Ureaplasma alone may cause limited inflammation and minimal tissue injury in the early phase of infection but may promote a mild chronic inflammatory response in the later phase of infection (days 14 to 28), similar to the process that occurs in human newborns.

show abstract

Section: Discussionsupporting

confidence: 85%

Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 95%

See 1 more Smart Citation

Characterization of a Murine Model ofUreaplasma urealyticumPneumonia

Viscardi¹,

Kaplan²,

et al. 2002

Infect Immun

View full text Add to dashboard Cite

show abstract

“…HSV, herpes simplex virus; CMV, cytomegalovirus; GBS, group B Streptococcus; MRSA, methicillin-resistant Staphylococcus aureus; PCL, positive control ladder; NC, negative control; PC, positive control. Quinn PA et al 6 Waites KB et al 7 Brus F et al 8 Our (Figure 1b). The cause of death was determined as intrauterine pneumonia, because the bilateral lungs already had severe infiltration of inflammatory cells soon after birth.…”

Section: Introductionmentioning

confidence: 76%

“…1,5 To our knowledge, only three cases of congenital pneumonia caused by intrauterine infection of Ureaplasma in term newborns have been previously reported (Table 1). [6][7][8] The onset of symptoms was within 1 day after birth in all of the three cases. All cases died despite intensive care, and postmortem microscopic examinations showed severe pneumonia as the cause of death.…”

Section: Discussionmentioning

confidence: 99%