Intrauterine administration of autologous peripheral blood mononuclear cells increases clinical pregnancy rates in frozen/thawed embryo transfer cycles of patients with repeated implantation failure

Okitsu, Osamu; Kiyokawa, Machiko; Oda, Takashi; Miyake, Kaoru; Sato, Yukiyasu; Fujiwara, Hiroshi

doi:10.1016/j.jri.2011.07.001

Cited by 72 publications

(87 citation statements)

References 23 publications

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“…Human peripheral blood HSC transplantation is now a well established curative treatment for patients with various hematologic malignancies [131], and techniques that exploit the multi-differentiation potential of PBMCs could lead to the isolation of cells for the treatment of presently incurable non-hematologic diseases as well [132-135]. However, many preclinical studies are still needed to better characterize the PBMC population and understand the biological mechanism of their multi-differentiation, and to further define their differentiation capacity, transplantability and biosafety.…”

Section: Resultsmentioning

confidence: 99%

The multi-differentiation potential of peripheral blood mononuclear cells

Zhang

Huang

2012

Stem Cell Res Ther

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Peripheral blood is a large accessible source of adult stem cells for both basic research and clinical applications. Peripheral blood mononuclear cells (PBMCs) have been reported to contain a multitude of distinct multipotent progenitor cell populations and possess the potential to differentiate into blood cells, endothelial cells, hepatocytes, cardiomyogenic cells, smooth muscle cells, osteoblasts, osteoclasts, epithelial cells, neural cells, or myofibroblasts under appropriate conditions. Furthermore, transplantation of these PBMC-derived cells can regenerate tissues and restore function after injury. This mini-review summarizes the multi-differentiation potential of PBMCs reported in the past years, discusses the possible mechanisms for this multi-differentiation potential, and describes recent techniques for efficient PBMC isolation and purification.

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Section: Resultsmentioning

confidence: 99%

The multi-differentiation potential of peripheral blood mononuclear cells

Zhang

Huang

2012

Stem Cell Res Ther

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“…The intrauterine administration of autologous PBMC, which was activated by incubation with hCG for 48 h, effectively improved the implantation, clinical pregnancy, and live birth rates of patients with repeated in vitro fertilization failure. Later, Okitsu et al [41] found that intrauterine administration of freshly isolated PBMC effectively improved embryo implantation in patients receiving frozen/thawed embryo transfer after three or more repeated in vitro fertilization failures. We previously proposed that the intrauterine administration of hCG-activated murine PBMC could promote embryonic implantation by regulating endometrial receptivity, providing a possible mechanism of this new immunotherapy [12].…”

Section: Discussionmentioning

confidence: 99%

HCG-Activated Human Peripheral Blood Mononuclear Cells (PBMC) Promote Trophoblast Cell Invasion

Yan

Yin

et al. 2015

PLoS ONE

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Successful embryo implantation and placentation depend on appropriate trophoblast invasion into the maternal endometrial stroma. Human chorionic gonadotropin (hCG) is one of the earliest embryo-derived secreted signals in the peripheral blood mononuclear cells (PBMC) that abundantly expresses hCG receptors. The aims of this study were to estimate the effect of human embryo–secreted hCG on PBMC function and investigate the role and underlying mechanisms of activated PBMC in trophoblast invasion. Blood samples were collected from women undergoing benign gynecological surgery during the mid-secretory phase. PBMC were isolated and stimulated with or without hCG for 0 or 24 h. Interleukin-1β (IL-1β) and leukemia inhibitory factor (LIF) expressions in PBMC were detected by enzyme-linked immunosorbent assay and real-time polymerase chain reaction (PCR). The JAR cell line served as a model for trophoblast cells and was divided into four groups: control, hCG only, PBMC only, and PBMC with hCG. JAR cell invasive and proliferative abilities were detected by trans-well and CCK8 assays and matrix metalloproteinase (MMP)-2 (MMP-2), MMP-9, vascular endothelial growth factor (VEGF), tissue inhibitor of metalloproteinase (TIMP)-1, and TIMP-2 expressions in JAR cells were detected by western blotting and real-time PCR analysis. We found that hCG can remarkably promote IL-1β and LIF promotion in PBMC after 24-h culture. PBMC activated by hCG significantly increased the number of invasive JAR cells in an invasion assay without affecting proliferation, and hCG-activated PBMC significantly increased MMP-2, MMP-9, and VEGF and decreased TIMP-1 and TIMP-2 expressions in JAR cells in a dose-dependent manner. This study demonstrated that hCG stimulates cytokine secretion in human PBMC and could stimulate trophoblast invasion.

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“…PBMCs were found to produce cytokines and angiogenic factors necessary for implantation [56]. A study in humans has indicated that intrauterine infusion of PBMCs could help to improve the clinical pregnancy rate in patients with repeated implantation failure during in vitro fertilization-embryo transfer (IVF-ET) procedure [62], indicating that leucocytes play important role in ensuring the success of human embryo implantation.…”

Section: Lif Role In Leukocyte Recruitment During Implantationmentioning

confidence: 99%

Leukemia Inhibitory Factor: Roles in Embryo Implantation and in Nonhormonal Contraception

Salleh

Giribabu

2014

The Scientific World Journal

107

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Leukaemia inhibitory factor (LIF) plays an indispensible role in embryo implantation. Aberrant LIF production is linked to implantation failure. LIF regulates multiple processes prior to and during implantation such as uterine transformation into a receptive state, decidualization, blastocyst growth and development, embryo-endometrial interaction, trophoblast invasion, and immune modulation. Due to its critical role, LIF has been a target for a nonhormonal contraception. In this review, we summarize up-to-date information on the role of LIF in implantation and its role in contraception.

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Intrauterine administration of autologous peripheral blood mononuclear cells increases clinical pregnancy rates in frozen/thawed embryo transfer cycles of patients with repeated implantation failure

Cited by 72 publications

References 23 publications

The multi-differentiation potential of peripheral blood mononuclear cells

The multi-differentiation potential of peripheral blood mononuclear cells

HCG-Activated Human Peripheral Blood Mononuclear Cells (PBMC) Promote Trophoblast Cell Invasion

Leukemia Inhibitory Factor: Roles in Embryo Implantation and in Nonhormonal Contraception

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