Intratypic variants of human papillomavirus type 16 and risk of cervical Neoplasia in Taiwan

Chang, Yu-Hao; Chen, Hui‐Chi; Pan, Mei‐Hung; Lee, Bor-Heng; You, San–Lin; Lin, Chien Ju; Chou, Yi-Chun; Hsieh, Chang‐Yao; Cheng, Yiping; Liaw, Kai–Li; Hsing, Ann W.; Schiffman, Mark; Chen, Chien-Jen

doi:10.1002/jmv.23651

Cited by 19 publications

(8 citation statements)

References 46 publications

(84 reference statements)

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“…Similarly, in most Asian studies, A4 variants have been most frequently observed in cervical cancer . In previous Asian studies, A1–3 variants showed a tendency toward a negative association with severe cervical lesions . This is concordant with our finding that A1–3 variants were negatively associated with cervical cancer compared to A4 or D variants.…”

Section: Discussionsupporting

confidence: 92%

“…Among the 92 isolates, 7521G>A (91.3%) was the most frequent substitution, followed by 7730A>C (59.8%), 7842G>A (59.8%), and 7781T>C (29.3%). Several nucleotide variations, including 7730A>C, 7842G>A, 178T>G, and 647A>G, were co‐linked in the A4 sublineage as previously reported by Chang et al . We discovered seven previously unreported nucleotide substitutions in the LCR.…”

Section: Resultssupporting

confidence: 83%

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Association of human papillomavirus type 16 and its genetic variants with cervical lesion in Korea

Park

Shin

Kim

et al. 2016

APMIS

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Association of human papillomavirus type 16 and its genetic variants with cervical lesion in Korea. APMIS 2016; 124: 950-957. Persistent human papillomavirus type 16 (HPV16) is the major risk factor for cervical cancer. HPV16 intratypic variants differ in their geographical distribution and oncogenic potential. This study aimed to analyze the distribution of HPV16 variants and their association with cervical lesion histopathology in Korean women. In total, 133 HPV16-positive cervical samples from women admitted to Seoul National University Boramae Hospital were analyzed by sequencing E6, E7, and L1 genes and the long control region (LCR), and the variant distribution according to cervical lesion grade was determined. Isolates were grouped into a phylogenetic lineage, and A1-3, A4, C, and D sublineages were detected in 54.1, 37.8, 0.7, and 7.4% of samples, respectively. The most commonly observed LCR variations were 7521G>A (91.5%), 7730A>C (59.6%), and 7842G>A (59.6%). Furthermore, A4 or D sublineage-positive women had a higher risk for cervical cancer than women who were positive for A1-3. Among HPV phylogenetic clusters, A1-3 was the predominant sublineage, and within A1-3, the 350G polymorphism was highly frequent. These results differed from those of previous studies in Korea and other Asian countries. The findings suggest that cervical neoplasia incidence in HPV16-infected patients could be affected by the distribution of HPV16 variants in the population.Key words: Human papillomavirus 16; variant; cervical cancer; cervical intraepithelial neoplasia; case-control study.Sue Shin, Department of Laboratory Medicine, Seoul National University Boramae Hospital, 156-707, 20 Boramaero-5-gil, Dongjak-gu, Seoul, Korea. e-mail: jeannie@snu.ac.krThe incidence rate of cervical cancer in Korea is higher than that in other developed countries. In 2012, the reported incidence was 14.2 cases per 100 000 women, with 3584 new cases and 889 deaths (1). Persistent infection by oncogenic human papillomaviruses (HPV) is strongly associated with the development of cervical neoplasia. Oncogenic HPV type 16 (HPV16) accounts for approximately 65% of cervical cancer worldwide (2) and is the most common HPV type associated with cervical cancer in Korea (3).The HPV oncogenes, E6 and E7, are consistently expressed in cervical cancers, and these genes are involved in HPV-mediated carcinogenesis (4). Currently, HPV16 intratypic variants, which have nucleotide sequence variations of ≤2% as compared with the HPV16 prototype, are classified into four major lineages based on whole-genome sequencing: A, which includes the A1-3 (previously named European), and A4 (Asian) sublineages; B (African 1); C (African 2); and D [including Asian-American (AA) and North American (NA)] (5). The long control region (LCR) adjacent to the downstream region of E6 contains the early promoter and regulatory elements involved in viral DNA replication and transcription (6). Epidemiological studies have reported that HPV16 variants confer different risks of vira...

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Section: Discussionsupporting

confidence: 92%

Section: Resultssupporting

confidence: 83%

Association of human papillomavirus type 16 and its genetic variants with cervical lesion in Korea

Park

Shin

Kim

et al. 2016

APMIS

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“…The prevalence of different variants depends on geographic location and ethnic background ( 14 ). As historically many areas on several different continents have been colonized by Europeans, European variants are therefore the most prevalent ( 5 , 15 ). The distribution of other intratypic variants for other HPV types has been of interest in more recent years, although overall it is rarely studied.…”

mentioning

confidence: 99%

Human papillomavirus variants among Inuit women in northern Quebec, Canada

Gauthier

Coutlée

Franco

et al. 2015

International Journal of Circumpolar Health

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BackgroundInuit communities in northern Quebec have high rates of human papillomavirus (HPV) infection, cervical cancer and cervical cancer–related mortality as compared to the Canadian population. HPV types can be further classified as intratypic variants based on the extent of homology in their nucleotide sequences. There is limited information on the distribution of intratypic variants in circumpolar areas.ObjectiveOur goal was to describe the HPV intratypic variants and associated baseline characteristics.DesignWe collected cervical cell samples in 2002–2006 from 676 Inuit women between the ages of 15 and 69 years in Nunavik. DNA isolates from high-risk HPVs were sequenced to determine the intratypic variant.ResultsThere were 149 women that were positive for HPVs 16, 18, 31, 33, 35, 45, 52, 56 or 58 during follow-up. There were 5 different HPV16 variants, all of European lineage, among the 57 women positive for this type. There were 8 different variants of HPV18 present and all were of European lineage (n=21). The majority of samples of HPV31 (n=52) were of lineage B. The number of isolates and diversity of the other HPV types was low. Age was the only covariate associated with HPV16 variant category.ConclusionsThese frequencies are similar to what was seen in another circumpolar region of Canada, although there appears to be less diversity as only European variants were detected. This study shows that most variants were clustered in one lineage for each HPV type.

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“…The long control region (LCR) is the most variable region within the HPV genome with as much as 5% variability within variants, and has been used to characterize HPV isolates of various types [23] , [24] , [25] . When a participant tested positive for HPV 16 at one of the two study visits, a paired sampled was identified for comparison of LCR sequence.…”

Section: Methodsmentioning

confidence: 99%

Redetection of human papillomavirus type 16 infections of the cervix in mid-adult life

Ermel

Shew

Imburgia

et al. 2018

Papillomavirus Research

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PurposeTo assess whether HPV 16 originally detected in adolescent women can be redetected in adulthood.MethodsA convenience sample of 27 adult women with known HPV 16 detection during adolescence was assessed for HPV 16 redetection. A comparison of the long control region (LCR) DNA sequences was performed on some of the original and redetected HPV 16 isolates.ResultsMedian age at reenrollment was 27.5 years (interquartile range of 26.7–29.6). Reenrollment occurred six years on average after the original HPV 16 detection. Eleven of 27 women had HPV 16 redetected. Some of these HPV 16 infections had apparently cleared during adolescence. LCR sequencing was successful in paired isolates from 6 women; in 5 of 6 cases the redetected HPV 16 isolates were identical to those detected during adolescence,ConclusionsHPV 16 may be episodically detected in young women, even over long time periods. HPV 16 redetection with identical LCR sequences suggests low-level persistent infection rather than true clearance, although newly acquired infection with an identical HPV 16 isolate cannot be excluded. However, this study suggests that a new HPV 16-positive test in a clinical setting may not indicate a new infection.

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Intratypic variants of human papillomavirus type 16 and risk of cervical Neoplasia in Taiwan

Cited by 19 publications

References 46 publications

Association of human papillomavirus type 16 and its genetic variants with cervical lesion in Korea

Association of human papillomavirus type 16 and its genetic variants with cervical lesion in Korea

Human papillomavirus variants among Inuit women in northern Quebec, Canada

Redetection of human papillomavirus type 16 infections of the cervix in mid-adult life

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