Intratypic differentiation of poliovirus strains by enzyme-linked immunosorbent assay (ELISA): poliovirus type 2 and poliovirus type 3

Glikmann, Graciela; Pedersen, Maria; Petersen, Ida Nymann

doi:10.1016/0166-0934(87)90107-8

Cited by 9 publications

(7 citation statements)

References 8 publications

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“…Intratypic differentiation is based on the detection of antigenic differences between vaccine strains and currently circulating wild PV strains, and it may be achieved by neutralization of infectivity with crossabsorbed polyclonal antisera specific for either Sabin vaccine strains or common wild strains (327,451,469) or panels of vaccine-derived and/or wild PV strain-specific monoclonal antibodies (85,101,113,189,327). The neutralization test involving polyclonal antisera has now been replaced by an enzyme immunoassay (131,327,443), which allows a more conservative use of antisera.…”

Section: Enterovirus Serotypingmentioning

confidence: 99%

Molecular Typing of Enteroviruses: Current Status and Future Requirements

et al. 1998

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Human enteroviruses have traditionally been typed according to neutralization serotype. This procedure is limited by the difficulty in culturing some enteroviruses, the availability of antisera for serotyping, and the cost and technical complexity of serotyping procedures. Furthermore, the impact of information derived from enterovirus serotyping is generally perceived to be low. Enteroviruses are now increasingly being detected by PCR rather than by culture. Classical typing methods will therefore no longer be possible in most instances. An alternative means of enterovirus typing, employing PCR in conjunction with molecular genetic techniques such as nucleotide sequencing or nucleic acid hybridization, would complement molecular diagnosis, may overcome some of the problems associated with serotyping, and would provide additional information regarding the epidemiology and biological properties of enteroviruses. We argue the case for developing a molecular typing system, discuss the genetic basis of such a system, review the literature describing attempts to identify or classify enteroviruses by molecular methods, and suggest ways in which the goal of molecular typing may be realized.

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Section: Enterovirus Serotypingmentioning

confidence: 99%

Molecular Typing of Enteroviruses: Current Status and Future Requirements

et al. 1998

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“…Four monoclonal antibodies, B1, B2, and B3 from ascitic fluids, and B4 from hybridoma cell supernatant, were characterized by SDS-PAGE/immunoblotting as described in Glikmann et al (1987) using purified influenza-A and influenza-B virions adjusted to the same protein concentration (50 pg/ml). Isotype determination of the monoclonal antibodies was carried out by means of isotyping reagent kits for mouse monoclonal antibodies (IS0-1 and IS0-2, St. Louis, MO, USA) according to the manufacturer's instructions.…”

Section: Characterization Of Monoclonal Antibodiesmentioning

confidence: 99%

Monoclonal antibodies for the rapid diagnosis of influenza-B virus infections by ELISA: production and characterization

Glikmann

Chen

Mordhorst

et al. 1995

Clinical and Diagnostic Virology

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“…Since then, newer strategies in microbiological diagnoses and molecular virology have added new possibilities for rapid and reliable ITD of poliovirus isolates. Cross‐absorbed intratype‐specific polyclonal rabbit antisera (PAbs) are used currently in an enzyme‐linked immunosorbent assay (ELISA) format [Osterhaus et al, 1983; Glikmann et al, 1984]. Accordingly, PCR and hybridization assays have been developed to enable specific characterization and sensitive detection of Sabin vaccine‐related strains [Takeda et al, 1994; De et al, 1995].…”

Section: Introductionmentioning

confidence: 99%

Comparative study of molecular and antigenic characterization for intratypic differentiation (ITD) of poliovirus strains

Adedeji

Okonko

Adu

2012

Journal of Medical Virology

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This study was designed to compare the sensitivity of a Sabin vaccine strain-specific PCR assay and an enzyme-linked immunosorbent assay with polyclonal cross-absorbed antisera (PAb-E) for intratypic differentiation (ITD) of polioviruses (PVs). These were used for the definitive characterization of the strains. Poliovirus strains isolated in L20B and RD cell lines were subjected to both PCR and ELISA. Both PCR and ELISA identified 3 (13.6%) out of 22 isolates, respectively as poliovirus Sabin 1. PCR identified 4 (18.2%) out of 22 isolates as poliovirus Sabin 2 and ELISA identified 2 (9.1%) out of 22 isolates as poliovirus Sabin 2. None of the two assay identified poliovirus Sabin 3. Both PCR and ELISA identified 12 (54.5%) out of 22 isolates, respectively as wild poliovirus (WPV) 1. None of the assays identified any of the isolates as WPV 2 and 3. Only PCR assay was able to identify the mixture of two poliovirus Sabin serotypes (a mixture of Sabin 1 and 2) and two mixtures of poliovirus Sabin 2 and 3. In this study, only ELISA was able to identified two invalid results. Invalid results observed in this study are of important practical implication to the emergence of vaccine-derived poliovirus. This may have epidemic potential. Hence, the two ITD assays are of paramount importance for identification of PVs. It is therefore recommended in line with WHO guideline that at least two methods be used for the ITD of poliovirus isolates, and each method should be based on a different principle (i.e., antigenic and genetic properties).

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Intratypic differentiation of poliovirus strains by enzyme-linked immunosorbent assay (ELISA): poliovirus type 2 and poliovirus type 3

Cited by 9 publications

References 8 publications

Molecular Typing of Enteroviruses: Current Status and Future Requirements

Molecular Typing of Enteroviruses: Current Status and Future Requirements

Monoclonal antibodies for the rapid diagnosis of influenza-B virus infections by ELISA: production and characterization

Comparative study of molecular and antigenic characterization for intratypic differentiation (ITD) of poliovirus strains

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