Intratunical injection of autologous adipose stromal vascular fraction reduces collagen III expression in a rat model of chronic penile fibrosis

Hakim, Lukman; Fiorenzo, Salvatore; Hedlund, Petter; Montorsi, Francesco; Bivalacqua, Trinity J.; Ridder, Dirk De; Weyne, Emmanuel; Ralph, David; Garaffa, Giulio; Muneer, Asıf; Joniau, Steven; Albersen, Maarten; Castiglione, Fabio

doi:10.1038/s41443-019-0136-9

Cited by 11 publications

(6 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Studies showed that both allogeneic and xenogenic ADSCs injection could decrease TGF-β1 induced urethral fibrosis with the downregulation of collagen I and collagen III 6 , 23 . Adipose tissue derived SVF, consisting of heterogeneous cell populations including ADSCs, is reported to be capable of preventing fibrotic changes of penile fibrosis in acute and chronic phase of Peyronie’s disease 9 , 24 . We previously found that local injection of SVF cells contributed to the attenuation of ischemia–reperfusion injury induced renal fibrosis 8 .…”

Section: Discussionmentioning

confidence: 99%

Effect of uncultured adipose-derived stromal vascular fraction on preventing urethral stricture formation in rats

Zhou

Yang

Zhao

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Urethral stricture (US) remains a challenging disease without effective treatment options due to the high recurrence rate. This study aims to evaluate the preventive effect of uncultured adipose derived stromal vascular fraction (SVF) on urethral fibrosis in a rat model of US. Results demonstrated that US rats displayed hyperechogenic urethral wall with a narrowed lumen compared with sham rats, while SVF rats exhibited less extensive urethral changes. By histology, US rats showed obvious submucosal fibrosis in the urethral specimens, while SVF rats exhibited mild submucosal fibrosis with less extensive tissue changes. Furthermore, US rats showed increased gene and protein expression of collagen I (2.0 ± 0.2, 2.2 ± 0.2, all were normalized against GAPDH, including the following), collagen III (2.5 ± 0.3, 1.2 ± 0.1), and TGFβ1R (2.8 ± 0.3, 1.9 ± 0.2), while SVF cells administration contributed to decreased gene and protein expression of collagen I (1.6 ± 0.2, 1.6 ± 0.2), collagen III (1.8 ± 0.4, 0.9 ± 0.1), and TGFβ1R (1.8 ± 0.3, 1.3 ± 0.2), in parallel with the improvement of vascularization and increased expression of VEGF (1.7 ± 0.1) and bFGF (3.1 ± 0.3). Additionally, SVF served anti-inflammatory effect through regulation of inflammatory cytokines and cells, accompanied with conversion of the macrophage phenotype. Our findings suggested that uncultured SVF presented an inhibitory effect on stricture formation at an early stage of urethral fibrosis.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of uncultured adipose-derived stromal vascular fraction on preventing urethral stricture formation in rats

Zhou

Yang

Zhao

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“… 36 Few articles in the sexual medicine field have reported the use of the ARRIVE checklist. 38 , 39 , 40 , 41 This concept is very important because similar to humans, several mammals display —in aging or because of various diseases—signs of ED and alterations of important regulatory pathways of erection. 24 The lack of crucial information can prevent the correct evaluation of the final results obtained.…”

Section: General Concepts Of Translational Research—the Animal Researmentioning

confidence: 99%

European Society for Sexual Medicine Consensus Statement on the Use of the Cavernous Nerve Injury Rodent Model to Study Postradical Prostatectomy Erectile Dysfunction

et al. 2020

Self Cite

View full text Add to dashboard Cite

Introduction Rodent animal models are currently the most used in vivo model in translational studies looking into the pathophysiology of erectile dysfunction after nerve-sparing radical prostatectomy. Aim This European Society for Sexual Medicine (ESSM) statement aims to guide scientists toward utilization of the rodent model in an appropriate, timely, and proficient fashion. Methods MEDLINE and EMBASE databases were searched for basic science studies, using a rodent animal model, looking into the consequence of pelvic nerve injury on erectile function. Main outcome measures The authors present a consensus on how to best perform experiments with this rodent model, the details of the technique, and highlight possible pitfalls. Results Owing to the specific issue—basic science—Oxford 2011 Levels of Evidence criteria cannot be applied. However, ESSM statements on this topic will be provided in which we summarize the ESSM position on various aspects of the model such as the use of the Animal Research Reporting In Vivo Experiments guideline and the of common range parameter for nerve stimulation. We also highlighted the translational limits of the model. Conclusion The following statements were formulated as a suggestive guidance for scientists using the cavernous nerve injury model. With this, we hope to standardize and further improve the quality of research in this field. It must be noted that this model has its limitations. Weyne E, Ilg MM, Cakir OO, et al. European Society for Sexual Medicine Consensus Statement on the Use of the Cavernous Nerve Injury Rodent Model to Study Postradical Prostatectomy Erectile Dysfunction. Sex Med 2020;8:327–337.

show abstract

“…However, it is also associated with a high variability of the SVF’s therapeutic potential among individuals, making it more complicated to standardize SVF-based treatments[ 116 ]. The use of SVF as treatment of urogenital disorders has been widely investigated in recent years in particular for preclinical studies for erectile disfunction and for Peyronie’s disease[ 117 - 120 ]. Moreover, a small number of early clinical studies using injected SVF for urogenital system related pathologies, like urinary incontinence, erectile disfunction, and Peyronie’s disease, have been performed in the last years[ 121 - 123 ].…”

Section: Towards Clinical Applicationsmentioning

confidence: 99%

Considerations for the clinical use of stem cells in genitourinary regenerative medicine

Caneparo

Sorroza-Martinez

Chabaud

et al. 2021

WJSC

View full text Add to dashboard Cite

The genitourinary tract can be affected by several pathologies which require repair or replacement to recover biological functions. Current therapeutic strategies are challenged by a growing shortage of adequate tissues. Therefore, new options must be considered for the treatment of patients, with the use of stem cells (SCs) being attractive. Two different strategies can be derived from stem cell use: Cell therapy and tissue therapy, mainly through tissue engineering. The recent advances using these approaches are described in this review, with a focus on stromal/mesenchymal cells found in adipose tissue. Indeed, the accessibility, high yield at harvest as well as anti-fibrotic, immunomodulatory and proangiogenic properties make adipose-derived stromal/SCs promising alternatives to the therapies currently offered to patients. Finally, an innovative technique allowing tissue reconstruction without exogenous material, the self-assembly approach, will be presented. Despite advances, more studies are needed to translate such approaches from the bench to clinics in urology. For the 21 st century, cell and tissue therapies based on SCs are certainly the future of genitourinary regenerative medicine.

show abstract

Intratunical injection of autologous adipose stromal vascular fraction reduces collagen III expression in a rat model of chronic penile fibrosis

Cited by 11 publications

References 42 publications

Effect of uncultured adipose-derived stromal vascular fraction on preventing urethral stricture formation in rats

Effect of uncultured adipose-derived stromal vascular fraction on preventing urethral stricture formation in rats

European Society for Sexual Medicine Consensus Statement on the Use of the Cavernous Nerve Injury Rodent Model to Study Postradical Prostatectomy Erectile Dysfunction

Considerations for the clinical use of stem cells in genitourinary regenerative medicine

Contact Info

Product

Resources

About