Intratumoral heterogeneity of esophageal squamous cell carcinoma

Walter, Dirk

doi:10.21037/jtd.2017.04.54

Cited by 1 publication

(1 citation statement)

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“…Heterogeneity mentioned the different morphology and genotype in distinct tumor cells, this phenomenon can happen between tumors and also within tumors which is called inter-tumor heterogeneity and intra-tumor heterogeneity respectively [4] and caused by both genetic and non-genetic factors in a variety of cancers as well as esophageal cancer [13].…”

Section: Discussionmentioning

confidence: 99%

HPV18 E6 and E7 Intratumour Heterogeneity in Esophageal Cancer

Khodahemmati¹,

Gaffar²,

Li³

et al. 2019

JCT

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The development of esophageal cancer accompanied by the presence of human papillomavirus (HPV) DNA into the host genome. By evaluating the expression of this virus for tumor cell origin and also their cell grows and migrations, we examined esophageal cancer clonality in the context of intra-tumor heterogeneity. In this research, we have checked the expression of HPV18 E6 and E7 in different single cell clones by the manual cell picking method in the HPV positive esophageal cancer (EC109), EC109 cell line used as a negative control, and Hela cell line used as the positive control. Quantitative real-time PCR (QRT-PCR) was run to detect the expression levels of HPV E6 and E7, Cell Counting Kit-8 (CCK-8) assay was used to examine cell proliferation, invasion assays performed using Costar chambers and wounding assay to study cell migrations in vitro. We investigated the intra-tumor heterogeneity of HPV E6 and E7 in esophageal cancer and the evaluation of the growth and migrations at the clonal level, using 10 single cell clones. In particular clones, C7 & C10 displayed a highly variable expression in both HPV E6 and E7 and weak in four clones (C1, C3, C4, and C9) consequently, the cell invasion, proliferation, and migration increase with increasing the level of HPV expression and inverse. In conclusion, the resulting based on single cell cloning showed the relationship between HPV and cell growth and migration in esophageal cancer. Future study in HPV DNA integration needed to explore the mains specific integration site of HPV DNA in esophageal cancer and molecular monitoring of the HPV for future prevention researches and also effective therapeutic strategies.

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Section: Discussionmentioning

confidence: 99%