Intratumoral delivery of paclitaxel-loaded poly(lactic-co-glycolic acid) microspheres for Hep-2 laryngeal squamous cell carcinoma xenografts

Abstract

The introduction of induction chemotherapy provides an expectation of laryngeal function preservation without reduction in survival for patients with advanced laryngeal squamous cell carcinoma. The antitumor activity of conventional intravenous chemotherapy, however, is limited by systemic toxicity. The polymeric drug system delivered locally provides a novel modality of increasing therapeutic concentrations of drug for a prolonged period while decreasing systemic levels. In the current study, paclitaxel-loaded sustained-release microspheres were developed using poly(lactic-co-glycolic acid) as a drug carrier. Intratumoral administration of paclitaxel in the formulation of polymer showed enhanced efficacy against laryngeal squamous cell carcinoma in nude mice compared with conventional paclitaxel injection via the intratumoral or intraperitoneal route. No significant toxic reactions were observed in the experiment. Immunohistochemical findings indicated that paclitaxel exhibited antiangiogenic activity by inhibiting the expression of basic fibroblast growth factor and vascular endothelial growth factor within the tumor. Moreover, this effect could be better exploited via localized delivery of polymeric paclitaxel. In conclusion, direct administration of polymeric drug system at the tumor sites proved to be promising for the treatment of laryngeal carcinoma.

EGFR-targeted poly(ethylene glycol)-distearoylphosphatidylethanolamine micelle loaded with paclitaxel for laryngeal cancer: preparation, characterization andin vitroevaluation

“…Nanoformulations have been used to enhance water solubility and bioavailability of poorly soluble drugs. In previous studies, we have developed paclitaxel-loaded poly(lactic-co-glycolic acid) (PLGA) microspheres for Hep-2 laryngeal squamous cell carcinoma model (Xie et al, 2007). Compared with conventional paclitaxel injection, intratumoral administration of paclitaxel in this formulation showed enhanced efficacy against laryngeal squamous cell carcinoma in nude mice.…”

Co-encapsulation of magnetic Fe3O4 nanoparticles and doxorubicin into biodegradable PLGA nanocarriers for intratumoral drug delivery

“…[6][7][8] Intratumoral administration of chemotherapeutic agents is a potentially more effective modality to overcome the described limitation and this has been extensively evaluated using a number of anticancer drugs. 9,10 Such targeted delivery may realize drug localization within the tumor tissue and divert the drug from nontarget organs to improve toxicity and increase efficacy, while decreasing the incidence and the intensity of side effects.…”

“…It is proposed that a drug delivery system is required to ensure the drug is properly localized and that it is released in a controlled way. 11 Several polymeric drug delivery systems have been developed for intratumoral drug delivery, including hydrogels, 9 microparticles, 10 nanoparticles, 12 and nanofibers. 13 Magnetic nanoparticles (MNPs) have been investigated for decades in drug delivery systems because of their high magnetic responsiveness, biodeg radability, biocompatibility, high delivery efficiency and potential targeting function.…”

Effect of Docetaxel on the Surgical Tumor Microenvironment of Head and Neck Cancer in Murine Models

“…The mean (SD) tumor weight was 403 (413) mg. All mice in the control group (n=8) had to be humanely killed because of the excessive tumor burden by day 37 (Table 2). In the BMEC model ( Figure 4B), nodules grew rapidly in the control and treatment groups, and all of the mice had to be humanely killed after 28 …”