“…C57BL/6 mice were purchased from Jackson Laboratories (Bar Harbor, ME). The following utilized TCR-transgenic animals have been described previously: chicken ovalbumin-specific MHC class I (OT-I, OVA 257-264 , Va2/b5) [57] or MHC class II (OT-II, OVA 323-339 , Va2/b5 [58] and DO11.10, OVA 323-339 , Va13/ b8.2) [59,60]; LCMV-specific MHC class I (P14, gp33 [33][34][35][36][37][38][39][40][41] , Va2/b8.1 CD8 + T cells) [61], and male antigen-specific MHC class I H-Y (Va3/b8.2) [14]. To exclude endogenous TCR rearrangement, OT-I, P14 and H-Y mice were crossed onto the RAG2 -/-background.…”