Intrathecally produced antibodies to Borrelia burgdorferi measured by IgG-capture ELISA

Stedingk, Lars‐Victor von; Stedingk, Marit Von; Hådemark, H.-G.; Brauner, Annelie; Kindstrand, E.; Hovmark, A; Asbrink, E

doi:10.1016/0888-0786(90)90029-n

Cited by 9 publications

(7 citation statements)

References 15 publications

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“…Year and quarter of each year results were confirmed with EIA and hemagglutination inhibition test for IgM [Hofmann et al, 1979;Kunz and Hofmann, 19711. In 146 cases both serum and CSF were available from the day of admission for an EIA for detection of intrathecally produced IgG and IgM antibodies to Borrelia burgdorferi [von Stedingk et al, 1990;Olsson et al, 19911. In 6 cases with clinical suspicion of HSV encephalitis EIA for intrathecal response against HSV was carried out [Mathiesen et al, 19881.…”

mentioning

confidence: 99%

Early diagnosis of enteroviral meningitis by detection of specific IgM antibodies with a solid‐phase reverse immunosorbent test (SPRIST) and μ‐capture EIA

Glimåker

Samuelson

Magnius³

et al. 1992

Journal of Medical Virology

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A solid-phase reverse immunosorbent test (SPRIST) and a mu-capture enzyme immunosorbent assay (EIA) for detection of enterovirus-specific IgM antibodies were evaluated for enterovirus diagnosis of aseptic meningitis in 160 consecutive patients from whom enterovirus (11 different serotypes) were isolated in 64. In patients with an enterovirus isolate and/or four-fold titre rise in the complement fixation test (CFT) for enterovirus, specific enterovirus IgM antibodies were detected on the day of admission to hospital in 48% by SPRIST and in 50% by EIA and 4-6 days after onset of symptoms in 71% by SPRIST and 79% by EIA. A significant increase in titre was observed between serum sampled on the day of admission and 2 days later in 38% by SPRIST and in 41% by EIA. These results indicate that the IgM antibody response appears early in the course of aseptic meningitis. Since both SPRIST and EIA provide rapid results the tests may be of differential diagnostic value and the IgM antibody kinetics may be utilized for diagnosis during the acute phase of aseptic meningitis. With optimized serum sampling the positive outcome was 76% in SPRIST and 82% in EIA among patients with positive virus isolation and/or CFT for enterovirus. In 67 patients virus isolation and CFT for enterovirus yielded negative results as well as all non-enteroviral diagnostic tests. Thirty-eight of these patients were positive by SPRIST and/or EIA and in half of these 38 a significant titre rise and/or fall in SPRIST and/or EIA was recorded. The majority of these IgM-positive patients became ill in the late summer or autumn, i.e., the "enterovirus season."(ABSTRACT TRUNCATED AT 250 WORDS)

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mentioning

confidence: 99%

Early diagnosis of enteroviral meningitis by detection of specific IgM antibodies with a solid‐phase reverse immunosorbent test (SPRIST) and μ‐capture EIA

Glimåker

Samuelson

Magnius³

et al. 1992

Journal of Medical Virology

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“…The convalescent sera were analysed for Epstein-Barr virus (EBV) infection by ox blood hemolysis antibody test (confirmed by immunofluorescence of EBV viral capsid antigen specific IgM), human immunodeficiency virus (HIV) infection by EIA (Abbott Laboratories, Chicago, Illinois, USA), and tick-borne encephalitis (TBE) by EIA-IgG (FSME-Quick; Immuno AG, Vienna, Austria). Positive TBE results were confirmed with EIA and hemagglutination inhibition test for TBE specific IgM activity [Kunz and Hofmann, 1971;Hofmann et al, 19791. In 115 cases both serum and CSF were available from the day of admission for an EIA for detection of intrathecally produced IgG-and IgM-antibodies to B. burgdorferi [von Stedingk et al, 1990;Olsson et al, 19911. In four cases with clinical suspicion of HSV, encephalitis EIA tests for intrathecal response against HSV were performed.…”

Section: Serological Assays For Non-enteroviral Etiologiesmentioning

confidence: 99%

Diagnosis of enteroviral meningitis with IgG‐EIA using heat‐treated virions and synthetic peptides as antigens

Samuelson

Glimåker

Skoog

et al. 1993

Journal of Medical Virology

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Two recently developed enzyme immunosorbent assays (EIA) for the detection of significant titre increases in enteroviral IgG-antibodies were evaluated as diagnostic tools in 127 etiologically well-characterized patients with aseptic meningitis. One assay was based on heat-treated virions (H-EIA) and one on synthetic peptides (P-EIA) as antigens. The sensitivities, with virus isolation as reference method, were 0.67 by H-EIA and 0.62 by P-EIA, which were higher than by a routinely used complement fixation test (CFT, 0.51) but somewhat lower than the sensitivities found by two previously presented IgM-assays, mu-capture EIA, and solid-phase reverse immunosorbent test (SPRIST). The specificities of the two IgG-EIA techniques and CFT were apparently high, whereas the two IgM-assays showed positive reactions in some non-enteroviral cases. A relatively rapid increase in enteroviral IgG-antibodies was apparent using H-EIA and P-EIA. The two IgG-EIA tests contributed with considerable additional etiological information since significant IgG-rises were obtained in 13 patients by H-EIA and in 19 by P-EIA, respectively, out of the 56 individuals in whom enterovirus isolation was negative and a non-enteroviral diagnosis was not found. Thus, detection of enteroviral IgG-antibodies by H-EIA and P-EIA seems to be a valuable alternative to CFT for the routine diagnosis of enteroviral meningitis. The IgM-assays, mu-capture EIA, and SPRIST, may allow a relatively rapid report of an enteroviral infection. However, since both the IgM-tests are hampered by incomplete specificities, a confirmation of positive results by an IgG-assay should be carried out.

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“…The procedure outlined here was first described at the Fourth International Conference on Lyme borreliosis in I990 by two research groups (114,327). With this method it was possible to confirm the diagnosis of neuroborreliosis.…”

Section: Igm Antibodies To Borrelia Burgdorferimentioning

confidence: 99%

Abstract

1993

Acta Paediatrica

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Intrathecally produced antibodies to Borrelia burgdorferi measured by IgG-capture ELISA

Cited by 9 publications

References 15 publications

Early diagnosis of enteroviral meningitis by detection of specific IgM antibodies with a solid‐phase reverse immunosorbent test (SPRIST) and μ‐capture EIA

Early diagnosis of enteroviral meningitis by detection of specific IgM antibodies with a solid‐phase reverse immunosorbent test (SPRIST) and μ‐capture EIA

Diagnosis of enteroviral meningitis with IgG‐EIA using heat‐treated virions and synthetic peptides as antigens

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