Intrathecal synthesis in particular types of multiple sclerosis

Abstract

Aim. The aim of this study was to determine whether there were any differences in intrathecal synthesis of immunoglobulin G (IgG) (IgG index) and number of oligoclonal bands (OCB) among particular types of multiple sclerosis (MS). Methods. 120 cerebrospinal fluid (CSF) samples were examined from 29 clinically isolated syndrome (CIS) patients and 91 MS patients (77 patients with relapsing-remitting MS (RR), 6 patients with primary progressive course of the disease (PP) and 8 patients in secondary progression (SP)); mean age = 42 years (range = 18 to 70 years). Albumin and IgG in serum and CSF was evaluated using nephelometry; an albumin quotient (CSF albumin/serum albumin), an IgG quotient (CSF IgG/serum IgG) and an IgG index (IgG quotient / albumin quotient) were then calculated. OCB were assessed using isoelectric focusing (IEF) on agarose gel, followed by immunoblotting. All patients were evaluated using the Kurtzke Expanded Disability Status Scale (EDSS). Results. No statistically significant differences between the IgG index and OC bands relative to particular types of MS were found. Further, there were no significant correlations between EDSS values and intrathecal synthesis (IgG index: QIgG / Qalbumin) and OC bands. Conclusion.No difference in intrathecal synthesis (IgG index) and the number of OCB between different types of MS was confirmed.

Intrathecal BCR transcriptome in multiple sclerosis versus other neuroinflammation: Equally diverse and compartmentalized, but more mutated, biased and overlapping with the proteome

“…For similar reasons we included some MS patients with long disease duration who still had intrathecal synthesis of IgG. Disease duration does not seem to influence the degree of intrathecal IgG synthesis in MS [4,36], which might rather be determined by genetic factors [37]. It is possible that a study restricted to MS patients with shorter disease duration or more active inflammation would have shown a less stringent compartmentalization of the B-cell response.…”

Increased cerebrospinal fluid neurofilament light chain in central nervous system inflammatory demyelinating disease

“…In addition, the CSF NFL level in the CIS and MS groups was not related to CSF OCB, and the CSF NFL level in the NMOSD group was not related to serum AQP4-Ab. Some scholars found that whether the OCB was positive or not was related to the EDSS score (Sladkova et al, 2014) in MS patients, and most scholars believe that the effect of serum AQP4-Ab positivity on the EDSS score and clinical relapse is not yet clear in NMOSD patients (Chanson et al, 2013;Jarius et al, 2008), suggesting that the CSF NFL level is a biological indicator of the degree of disability and disease activity, independent of CSF OCB and serum AQP4-Ab.…”

Comparative characteristic of laboratory indicators for encephalitis, disseminated encephalomyelitis and multiple sclerosis in children

“…В современной литературе роль и значение ИИС IgG для диагностики по-прежнему дискутируются. Отмечается, что активация интратекального синтеза иммуноглобулинов наблюдается не только при РС, но и при других воспалительных и хронических инфекциях ЦНС [7,13,15]. В работе V. Sladkova и соавт.…”

“…В работе V. Sladkova и соавт. [15] не выявлено связи между показателями по шкале EDSS и интратекальным синтезом IgG, при этом отмечено, что только 72 % пациентов с РС имеют увеличенный индекс IgG. В работе A. Abdelhak и соавт.…”

“…Клинико-лабораторные показатели при диссеминированном энцефаломиелите. В диагностике ДЭМ учитывались международные критерии [15]. Все пациенты имели очаговые изменения на МРТ, локализующиеся преимущественно в белом веществе ЦНС.…”