2009
DOI: 10.1002/ana.21802
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Intrathecal pathogenic anti–aquaporin‐4 antibodies in early neuromyelitis optica

Abstract: Objective-The serum of most neuromyelitis optica (NMO) patients contains autoantibodies (NMO-IgGs) directed against the aquaporin-4 (AQP4) water channel located on astrocyte foot processes in the perivessel and subpial areas of the brain. Our objectives were to determine the source of central nervous system (CNS) NMO-IgGs and their role in disease pathogenesis.Methods-Fluorescence activated cell sorting and single-cell reverse transcriptase PCR were used to identify overrepresented plasma cell immunoglobulin (… Show more

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Cited by 526 publications
(588 citation statements)
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“…Single CSF mononuclear cells (MNCs) were prepared as described previously 1. Peripheral blood was collected in CPT tubes and mononuclear cells isolated according to the manufacturer's instructions (BD Vacutainer, CPT cell preparation tubes with sodium acetate) and cryopreserved at −80°C for later sample analysis.…”
Section: Methodsmentioning
confidence: 99%
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“…Single CSF mononuclear cells (MNCs) were prepared as described previously 1. Peripheral blood was collected in CPT tubes and mononuclear cells isolated according to the manufacturer's instructions (BD Vacutainer, CPT cell preparation tubes with sodium acetate) and cryopreserved at −80°C for later sample analysis.…”
Section: Methodsmentioning
confidence: 99%
“…CSF CD19 + CD138 + plasmablast heavy‐ (VH) and light‐chain (VL) variable region sequences were recovered by RT‐PCR and DNA sequencing as described previously 1, 7. Recombinant antibodies were produced in HEK293 cells (Invitrogen, Carlsbad, CA, R620‐07)7 and reactivity tested via immunofluorescence for binding to a permanent cell line expressing M23‐AQP4 8…”
Section: Methodsmentioning
confidence: 99%
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