Intrathecal Adenosine following Spinal Nerve Ligation in Rat

Bantel, Carsten; Tobin, Joseph R.; Li, Xinhui; Childers, Steven R.; Chen, Shao Rui; Eisenach, James C.

doi:10.1097/00000542-200201000-00022

Cited by 19 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We used a much lower dose of ATP than the doses of adenosine used for treatment of neuropathic pain in 13.0 (0-40)* Paroxysmal pain VAS (mm) 46.9 (0-69) 17.5 (0-60)* Allodynia VAS (mm) 49.5 (0-80) 15.6 (0-70)* Tactile hypesthesia (grade) 1.1 (0-2) 0.6 (0-2) a Values are median (range); n ϭ 8. Some values were tested for the effect of a single control infusion in terms of multiple infusions actually, so there are differences in baseline VAS * P Ͻ 0.05 previous studies [9,11,15].…”

Section: Discussionmentioning

confidence: 99%

“…ATP is metabolized rapidly to adenosine 5Ј-diphosphate or adenosine by ecto-ATPases [6], and adenosine has been suggested to be effective for neuropathic pain [7][8][9][10]. Some studies have shown improvement in neuropathic pain after administration of adenosine via an action on A1 receptors in both animals and humans [10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Systemic ATP infusion improves spontaneous pain and tactile allodynia, but not tactile hypesthesia, in patients with postherpetic neuralgia

et al. 2004

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Systemic ATP infusion improves spontaneous pain and tactile allodynia, but not tactile hypesthesia, in patients with postherpetic neuralgia

et al. 2004

View full text Add to dashboard Cite

show abstract

“…In a more recent study, Bantel et al (31) characterized adenosine receptors in the lumbar and thoracic levels in the rat spinal cord and also reported a single binding site for the A 1 receptor. However, the B max reported in their study is significantly higher than that in this study (probably because of the areas in the cord that the investigators focused on), suggesting that there is a higher density of adenosine A 1 receptors in the lumbar and thoracic regions than in the cervical spinal cord.…”

Section: Discussionmentioning

confidence: 99%

Changes in the Biochemical Profiles of Mid-Cervically Located Adenosine A1 Receptors After Repeated Theophylline Administration in Adult Rats

Saharan

Nantwi

2006

The Journal of Spinal Cord Medicine

View full text Add to dashboard Cite

Background/Objective: Adenosine A 1 receptors localized in the phrenic motoneurons (PMNs), where the axons of the descending bulbospinal respiratory make synaptic contacts, may be involved in theophyllineinduced respiratory-related activity in rats. The objective of this study was to characterize the biochemical profiles of adenosine A 1 receptors in 2 groups of rats: (a) naïve and (b) theophylline-treated (3-day oral administration).Methods: Biochemical binding characteristics of adenosine A 1 receptors in the C3 to C5 (PMN) of adult rats were assessed in naïve (n ¼ 6) and theophylline-treated animals (n ¼ 6) using [ 3 H]-DPCPX (10 pmol/L to 30 nmol/L), the specific adenosine A 1 receptor antagonist in saturation-binding assays. Competition assays used theophylline as the competing ligand (20 mmol/L to 20 pmol/L), and protein concentration was determined with the Bradford assay using a range of standards (0.016-1.0 mg/mL). Results:In saturation-binding assays in naïve animals, the A 1 receptor was characterized by a single binding site with B max and K d values of 256.00 6 32.13 fmol/mg protein and 2.89 6 0.45 nmol/L, respectively. Analysis of the isotherm in theophylline-treated animals showed 1 site with B max and K d values of 219.00 6 26.3 fmol/mg protein and 0.60 6 0.21 nmol/L, respectively, and a second site characterized by B max and K d values of 492.6 6 3.15 fmol/mg protein and 14.09 6 2.06 n mol/L, respectively.Conclusions: Theophylline administration revealed 2 binding sites on receptors (characterized by the specific adenosine A 1 antagonist, [ 3 H]-DPCPX) located in the vicinity of phrenic motoneurons (C3-C5). Alteration of the receptor profiles after theophylline may underlie the respiratory-related actions of the drug. Spinal Cord Med. 2006;29:520-526 J

show abstract

“…It is reported that there was no evidence for up-regulation in spinal A1 receptors after spinal nerve ligation and that spinal cord adenosine was decreased after spinal nerve ligation (21). …”

Section: Discussionmentioning

confidence: 99%

The Interaction of Gabapentin and N6-(2-phenylisopropyl)-adenosine R-(-)isomer (R-PIA) on Mechanical Allodynia in Rats with a Spinal Nerve Ligation

Park

Jun

2008

J Korean Med Sci

View full text Add to dashboard Cite

We examined the antiallodynic interaction between gabapentin and adenosine A1 receptor agonist, N6-(2-phenylisopropyl)-adenosine R-(-)isomer (R-PIA), in a rat model of nerve ligation injury. Rats were prepared with ligation of left L5-6 spinal nerves and intrathecal catheter implantation for drug administration. Mechanical allodynia was measured by applying von Frey filaments. Gabapentin and R-PIA were administered to obtain the dose-response curve and the 50% effective dose (ED50). Fractions of ED50s were administered concurrently to establish the ED50 of the drug combination. The drug interaction between gabapentin and R-PIA was analyzed using the isobolographic method. Adenosine A1 receptor antagonist was administered intrathecally to examine the reversal of the antiallodynic effect. Locomotor function changes were evaluated by rotarod testing. Intrathecal gabapentin and R-PIA and their combination produced a dose-dependent antagonism against mechanical allodynia without severe side effects. Intrathecal gabapentin synergistically enhanced the antiallodynic effect of R-PIA when coadministered. There were no significant changes in rotarod performance time, except gabapentin 300 μg. In the combination group, the maximal antiallodynic effect was reversed by A1 adenosine receptor antagonist. These results suggest that activation of adenosine A1 receptors at the spinal level is required for the synergistic interaction on the mechanical allodynia.

show abstract

Intrathecal Adenosine following Spinal Nerve Ligation in Rat

Cited by 19 publications

References 0 publications

Systemic ATP infusion improves spontaneous pain and tactile allodynia, but not tactile hypesthesia, in patients with postherpetic neuralgia

Systemic ATP infusion improves spontaneous pain and tactile allodynia, but not tactile hypesthesia, in patients with postherpetic neuralgia

Changes in the Biochemical Profiles of Mid-Cervically Located Adenosine A1 Receptors After Repeated Theophylline Administration in Adult Rats

The Interaction of Gabapentin and N6-(2-phenylisopropyl)-adenosine R-(-)isomer (R-PIA) on Mechanical Allodynia in Rats with a Spinal Nerve Ligation

Contact Info

Product

Resources

About