Intraspecific variation in Radopholus similis isolates assessed with restriction fragment length polymorphism and DNA sequencing of the internal transcribed spacer region of the ribosomal RNA cistron

Elbadri, G.A.A.; Ley, Paul De; Waeyenberge, Lieven; Vierstraete, Andy; Moens, Maurice; Vanfleteren, Jacques R.

doi:10.1016/s0020-7519(01)00319-8

Cited by 45 publications

(49 citation statements)

References 8 publications

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“…Therefore, classifying strains of the Taiwan isolates on the basis on these parameters is not feasible. The Taiwan isolates are highly aberrant and divergent members of the same species, as described by Elbadri et al (2002) for the parasitic nematode Radopholus similis.…”

Section: Discussionmentioning

confidence: 87%

“…This means that morphology, i.e. tomont size, does not always support phylogeny based on the rDNA sequence, nor are developmental characteristics always consistent with molecular relationships (Elbadri et al 2002). Hence, as suggested by Thompson & Lymbery (1990), phenotypic variation in some parasites does not always appear to have a genetic basis.…”

Section: Discussionmentioning

confidence: 94%

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Characterization of Cryptocaryon irritans, a parasite isolated from marine fishes in Taiwan

Yambot¹,

Song²,

Sung³

2003

Dis. Aquat. Org.

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The ciliated protozoan parasite Cryptocaryon irritans infecting marine fishes in Taiwan is described. Developmental characteristics and sequences of the ribosomal DNA regions such as part of 18 S, the entire first internal transcribed spacer, and part of 5.8 S of various Taiwan isolates of C. irritans were investigated. A total of 5 isolates was obtained from different fish-host species and localities, the majority from cultured fish species. C. irritans from Taiwan is able to shift its developmental characteristics, i.e. from non-adherent to adherent tomonts, from individualistic to aggregateforming tomonts, from infection of the gills only to infection of the gills and body. Thus, it is not possible to classify strains of C. irritans on the basis of these parameters. Premature tomonts that developed from dead fishes were able to produce theronts that could infect fish host. Isolates from Pingtung and the USA had identical nucleotide sequences while an isolate from Malaysia was identical to an Israel isolate. Percentage variation among pairs of Taiwan isolates showed a higher degree of variation than isolate sequences listed in GenBank. Sequence analysis revealed highly aberrant isolates in Taiwan, and a phylogenetic tree distinguished a marine and a low-salinity variant. C. irritans from marine fishes in Taiwan, therefore, display some characteristics not previously reported. Since manipulation of salinity in brackishwater ponds and marine cage sites is not feasible, there is a need to develop new strategies for the control and prevention of cryptocaryoniasis.

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 94%

Characterization of Cryptocaryon irritans, a parasite isolated from marine fishes in Taiwan

Yambot¹,

Song²,

Sung³

2003

Dis. Aquat. Org.

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“…Blaxter et al 1998;Bhadury et al 2006a, b;Creer et al 2010) and 28S rRNA genes (e.g. Litvaitis et al 2000;De Ley et al 2005;Pereira et al 2010) as well as internal transcribed spacers (ITS; Hugall et al 1999;Elbadri et al 2002;Félix et al 2014). Here, various studies showed that the D2-D3 region of the 28S rDNA represents one of the most suitable and promising markers for a valid nematode identification, which can be used to delimitate even closely related species (De Ley et al 2005;Pereira et al 2010).…”

Section: Introductionmentioning

confidence: 94%

The application of DNA sequence data for the identification of benthic nematodes from the North Sea

2014

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Nematodes or roundworms represent one of the most diverse and dominant taxon in marine benthic habitats. Whereas a morphological identification of many species is challenging, the application of molecular markers represents a promising approach for species discrimination and identification. In this study, we used an integrative taxonomic approach, combining both molecular and morphological methods, to characterize nematodes of distinct sex and ontogenetic stages from three sampling sites of the North Sea. Morphospecies were discriminated after first visual determination, followed by a molecular analysis of the nuclear 28S rDNA: D2-D3 marker. By linking each sequence to a morphological voucher, discordant morphological identification was subjected to a so-called reverse taxonomic approach. Molecular operational taxonomic units (MOTUs) and morphospecies were compared for all of the three sampling sites to assess concordance of methodology. In total, 32 MOTUs and 26 morphospecies were assigned, of which 12 taxa were identified as described species. Both approaches showed high concordance in taxon assignment (84.4 %) except for a cluster comprising various Sabatieria species. Our study revealed the high potential of the analyzed fragment as a useful molecular marker for the identification of the North Sea nematodes and highlighted the applicability of this combined taxonomic approach in general.

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“…Türkiye'den elde edilen 29 farklı kök-ur nematodu izolatı arasındaki farklılıklar ve benzerlikler AFLP yöntemi ile ortaya koyulmuştur (Devran et al, 2008). Önemli bitki paraziti nematod türlerini içeren Pratylenchus cinslerinin ayrımında da AFLP yöntemi sıklıkla ve yaygın olarak kullanılmıştır (Waeyenberge et al, 2000;Elbadri et al, 2002).…”

Section: Aflp (Amplified Fragment Length Polymorphism)unclassified

Nematod taksonomisinde kullanılan moleküler markörler

Gözel¹,

Yurt²,

Gözel³

2016

Türk. entomol. bült.

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Morphological characters and morphometric measurements are used for diagnosis and identification of nematodes. In some cases, reliable results can not be achieved by classic methods. Therefore, molecular markers have been applied to nematode identification. Molecular markers are methods based on DNA. In this article, information regarding to the most common molecular marker techniques including Restriction Fragment Length Polymorphism (RFLP), Amplified Fragment Length Polymorphism (AFLP), Microsatellites (SSR, STR, ISSR), Random Amplified Polymorphic DNA (RADP), Single Nucleotide Polymorphism (SNP) and DNA Sequence Analysis (mtDNA and rDNA) was given and the studies done by using these methods were reviewed.

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Intraspecific variation in Radopholus similis isolates assessed with restriction fragment length polymorphism and DNA sequencing of the internal transcribed spacer region of the ribosomal RNA cistron

Cited by 45 publications

References 8 publications

Characterization of Cryptocaryon irritans, a parasite isolated from marine fishes in Taiwan

Characterization of Cryptocaryon irritans, a parasite isolated from marine fishes in Taiwan

The application of DNA sequence data for the identification of benthic nematodes from the North Sea

Nematod taksonomisinde kullanılan moleküler markörler

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