Intraspecific variation in 16S rRNA gene of Mycoplasma synoviae determined by DNA sequencing

Buim, M. R.; Buzinhani, Melissa; Yamaguti, Maurício; Oliveira, Renata Cristina Gobbi de; Mettifogo, Elena; Timenetsky, Jorge; Ferreira, Antônio José Piantino

doi:10.1016/j.cimid.2008.07.005

Cited by 18 publications

(13 citation statements)

References 14 publications

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“…This high mutation rate, as indicated by this hypothesis that many 16S rRNA sequences which are highly conserved in Eubacteria are absent in Mycoplasmas (Rogser et al, 1985). Spontaneous mutations may occur when a lineage remains in the same flocks for long periods (Buim et al, 2010). The information of bacterial ribosomal RNA has been organised in operons.…”

Section: Discussionmentioning

confidence: 94%

“…Mycoplasmas, in general, show a more rapid evolutionary rate than other bacteria, which is reflected in variations of otherwise conserved nucleotides in Mycoplasma 16S rRNA (Weisburg et al, 1989). Buim et al (2010) showed different polymorphic patterns as polymorphisms and differences in nucleotides among some samples and genetic variability in strains from distinct Brazilian areas of poultry production. The results of this study support the findings of the previous report of phylogeny based on 16S rRNA gene which also showed the variations and differences in nucleotides and polymorphisms in M. synoviae 16S rRNA.…”

Section: Discussionmentioning

confidence: 99%

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Phylogenetic Analysis of Mycoplasma Synoviae Isolated from Commercial Iranian Chicken Farms Compared with Other Genebank Isolate Sequences Based on 16S rRNA Gene

Bayatzadeh

Pourbakhsh

Homayounimehr

et al. 2013

Avian Biology Research

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Mycoplasma synoviae (M. synoviae) is an important avian and extracellular pathogen which causes great economic losses in poultry industry. We have performed a phylogenetic analysis of M. synoviae isolates derived from commercial chicken farms in Iran based on 16S rRNA gene. Partial sequences of 16S rRNA genes obtained from 19 isolates of central region of Iran were amplified by PCR in 207bp fragment, then sequenced and next compared with the 16S rRNA gene of M. synoviae sequences that were available in GeneBank. Sequences were aligned using the ClustalW method, the UPGMA algorithm was used to create the phylogenetic tree. Phylogenetic analysis of these sequences demonstrated that all 19 M. synoviae isolated from Iran were most closely related to sequences of M. synoviae from Brazil. Variations, polymorphisms, and differences between nucleotides of all isolates were observed. The results of this study suggest that the close relationship, different molecular structure and heterogeneity among M. synoviae isolated may be explained by the transmission of mutations and variations between countries due to trading or to the stabilisation of spontaneous mutations because of regional conditions.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Phylogenetic Analysis of Mycoplasma Synoviae Isolated from Commercial Iranian Chicken Farms Compared with Other Genebank Isolate Sequences Based on 16S rRNA Gene

Bayatzadeh

Pourbakhsh

Homayounimehr

et al. 2013

Avian Biology Research

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show abstract

“…It is also important to establish whether the vaccine strain has colonised the respiratory mucosa so as to produce an efficient immune response to protect against wild-type disease. A number of PCR-based techniques have been reported for typing of M. synoviae strains, targeting the vlhA gene [4]-[7], 16S rRNA genes [8] or the 16S to 23S rRNA intergenic spacer region [9], [10]. Only a small number of these studies included the MS-H vaccine in their experiments.…”

Section: Introductionmentioning

confidence: 99%

High-Resolution Melting-Curve Analysis of obg Gene to Differentiate the Temperature-Sensitive Mycoplasma synoviae Vaccine Strain MS-H from Non-Temperature-Sensitive Strains

et al. 2014

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Temperature-sensitive (ts +) vaccine strain MS-H is the only live attenuated M. synoviae vaccine commercially available for use in poultry. With increasing use of this vaccine to control M. synoviae infections, differentiation of MS-H from field M. synoviae strains and from rarely occurring non-temperature-sensitive (ts –) MS-H revertants has become important, especially in countries where local strains are indistinguishable from MS-H by sequence analysis of variable lipoprotein haemagglutinin (vlhA) gene. Single nucleotide polymorphisms (SNPs) in the obg of MS-H have been found to associate with ts phenotype. In this study, four PCRs followed by high-resolution melting (HRM)-curve analysis of the regions encompassing these SNPs were developed and evaluated for their potential to differentiate MS-H from 36 M. synoviae strains/isolates. The nested-obg PCR-HRM differentiated ts + MS-H vaccine not only from field M. synoviae strains/isolates but also from ts – MS-H revertants. The mean genotype confidence percentages, 96.9±3.4 and 8.8±11.2 for ts + and ts – strains, respectively, demonstrated high differentiating power of the nested-obg PCR-HRM. Using a combination of nested-obg and obg-F3R3 PCR-HRM, 97% of the isolates/strains were typed according to their ts phenotype with all MS-H isolates typed as MS-H. A set of respiratory swabs from MS-H vaccinated specific pathogen free chickens and M. synoviae infected commercial chicken flocks were tested using obg PCR-HRM system and results were consistent with those of vlhA genotyping. The PCR-HRM system developed in this study, proved to be a rapid and reliable tool using pure M. synoviae cultures as well as direct clinical specimens.

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“…Além disso, Ms pode apresentar diferenças genotípicas pelas mutações espontâneas que podem acontecer quando a linhagem de aves permanece no mesmo plantel por longos períodos (BUIM et al, 2010).…”

Section: Pesquisa De Título De Anticorpos Em Aves De Subsistênciaunclassified

“…Cepas ou variantes de patógenos desconhecidos podem surgir com a presença de novos hospedeiros ou quando há reposição de aves (BUIM et al, 2010 Invariavelmente, os estudos apontam para as galinhas de "fundo de quintal" como reservatórios de agentes infecciosos, sendo consideradas disseminadoras para as aves silvestres, que por sua vez, tem livre acesso às granjas de produção e podem transmitir os agentes infecciosos das aves de subsistência para as aves comerciais e vice-versa (SALES et al, 2007;BUCHALA et al, 2006a;XAVIER;PASCAL;CRESPO, 2011;HAMILTON-WEST et al, 2012;SMITH et al, 2012).…”

Section: Pesquisa De Título De Anticorpos Em Aves De Subsistênciaunclassified

Detecção do vírus da Influenza Aviária, Paramyxovirus tipo 1 (vírus da Doença de Newcastle), Mycoplasma gallisepticum e Mycoplasma synoviae em aves silvestres e domésticas próximas às granjas avícolas comerciais nas regiões de Mogi das Cruzes e Louveira do Estado de São Paulo

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Intraspecific variation in 16S rRNA gene of Mycoplasma synoviae determined by DNA sequencing

Cited by 18 publications

References 14 publications

Phylogenetic Analysis of Mycoplasma Synoviae Isolated from Commercial Iranian Chicken Farms Compared with Other Genebank Isolate Sequences Based on 16S rRNA Gene

Phylogenetic Analysis of Mycoplasma Synoviae Isolated from Commercial Iranian Chicken Farms Compared with Other Genebank Isolate Sequences Based on 16S rRNA Gene

High-Resolution Melting-Curve Analysis of obg Gene to Differentiate the Temperature-Sensitive Mycoplasma synoviae Vaccine Strain MS-H from Non-Temperature-Sensitive Strains

Detecção do vírus da Influenza Aviária, Paramyxovirus tipo 1 (vírus da Doença de Newcastle), Mycoplasma gallisepticum e Mycoplasma synoviae em aves silvestres e domésticas próximas às granjas avícolas comerciais nas regiões de Mogi das Cruzes e Louveira do Estado de São Paulo

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